Abstract：Objective To conduct molecular identification of the common Siphonaptera species from the Alataw pass of China-Kazakhstan border based on the mitochondrial cytochrome C oxidase subunit I (CO Ⅰ) gene. Methods In May 2017, parasitic Siphonaptera on the body surface of rodents was collected from the Alataw pass of China-Kazakhstan border. DNA was extracted after morphological identification. The mitochondrial CO Ⅰ gene was amplified by PCR and sequenced. The sequencing results were analyzed by BLAST, and the phylogenetic tree was constructed using MAGE 6.0 software. Results The morphological and molecular biological identification showed 100% consistency between Echidnophaga oschanini and Ctenocephalides felis felis. Meanwhile, the mitochondrial CO Ⅰ sequences of Xenopsylla minax and Nosopsyllus laeviceps laeviceps were reported for the first time in the world. Molecular biological identification and morphological identification of the four species of Siphonaptera were in good agreement. The four species of Siphonaptera in China-Kazakhstan border were highly similar to Siphonaptera species in other countries. Conclusion DNA barcoding technique can identify the species of Siphonaptera effectively and provide a scientific basis for rapid and accurate identification of host and control of foreign medical vectors at ports.
尹小平, 刘戈, 王安东, 颜彬, 白岑, 田延河, 张兆冠, 赵姗姗, 王远志. DNA条形码技术在蚤类鉴定中的应用[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 561-563.
YIN Xiao-ping, LIU Ge, WANG An-dong, YAN Bin, BAI Cen, TIAN Yan-he, ZHANG Zhao-guan, ZHAO Shan-shan, WANG Yuan-zhi. Application of DNA barcoding technique in the molecular identification of Siphonaptera. Chines Journal of Vector Biology and Control, 2019, 30(5): 561-563.
2019, Vol. 30 
