中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (1): 38-41.DOI: 10.11853/j.issn.1003.8280.2018.01.010

• 论著 • 上一篇    下一篇

中国-哈萨克斯坦边境阿拉山口地区小兽携带汉坦病毒调查

尹小平1, 宋峰林2, 赵姗姗1,3, 田延河1, 巴特尔1, 程天立1, 张江国1, 高玉峰2   

  1. 1 阿拉山口出入境检验检疫局, 新疆 阿拉山口 833418;
    2 大连出入境检验检疫局, 辽宁 大连 833418;
    3 石河子大学医学院, 新疆 石河子 832000
  • 收稿日期:2017-09-30 出版日期:2018-02-20 发布日期:2018-02-20
  • 通讯作者: 高玉峰,Email:GAOYUFENG102238@163.com
  • 作者简介:尹小平,男,副研究员,主要从事口岸医学媒介监测工作,Email:yxpciq@163.com
  • 基金资助:
    国家质量监督检验检疫总局科技计划项目(2016IK264)

Hantavirus detected from small mammals in the China-Kazakhstan border

YIN Xiao-ping1, SONG Feng-lin2, ZHAO Shan-shan1,3, TIAN Yan-he1, BA Te-er1, CHENG Tian-li1, ZHANG Jiang-guo1, GAO Yu-feng2   

  1. 1 Alashankou Entry-Exit Inspection and Quarantine Bureau, Alashankou 833418, Xinjiang Uygur Autonomous Region, China;
    2 Dalian Entry-Exit Inspection and Quarantine Bureau;
    3 College of Medicine, Shihezi University
  • Received:2017-09-30 Online:2018-02-20 Published:2018-02-20
  • Supported by:
    Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject (No. 2016IK264)

摘要: 目的 了解中国-哈萨克斯坦(中哈)边境阿拉山口口岸地区捕获小兽携带汉坦病毒(HV)情况,为该地区对HV感染流行的防控提供依据。方法 2016年1-11月采用夹捕法和弓形夹法在中哈边境铁路沿线区域捕获小兽,随机选取95份小兽肺组织提取RNA并反转录为cDNA。采用HV特异性引物进行PCR检测,阳性产物进行测序、分析并利用MEGA 6.0软件构建分子遗传进化树。结果 95份小兽样本经PCR检测及测序发现阳性样本24份(其中大沙鼠11份,红尾沙鼠、子午沙鼠和柽柳沙鼠各4份,灰仓鼠1份),阳性率为25.26%。经DNAman比对分析发现5个不同HV基因型,同源性为98.41%;BLAST序列分析均与GenBank数据库中已登录的汉城型汉坦病毒(SEOV)的同源性达98.09%~98.33%。遗传进化树显示,与汉城型DPRK08聚为一支。结论 中哈边境阿拉山口铁路沿线区域首次在大沙鼠、红尾沙鼠、子午沙鼠、柽柳沙鼠和灰仓鼠中检测出SEOV,证实该地区有5种鼠类携带感染HV。

关键词: 小兽, 汉坦病毒, 聚合酶链式反应, 阿拉山口口岸, 中国-哈萨克斯坦边境

Abstract: Objective To determine the infection rate of hantavirus in rodents and provide basic data for the prevention and control of hemorrhagic fever with renal syndrome(HFRS) at China-Kazakhstan border railway extension line area. Methods Night trapping and arch clamp methods were applied to monitor population structure of rodents at China-Kazakhstan border railway extension line area, 95 mice's lung tissues were randomly selected to extract the RNA, and corresponding cDNA was synthesized by reverse transcription. All of 95 samples were detected by PCR targeting hantavirus. The amplified products were sequenced and analyzed. A phylogenetic tree was constructed using MEGA 6.0 software. Results Twenty-four (11 Rhombomys opimus, 4 Meriones libycus, 4 M. meridianus, 4 M. tamariscinus, 1 Cricetulus migratorius) samples were positive (24/95, 25.26%) for hantavirus. DNAman alignment analysis on 24 positive sequences, indicated that there are 6 different genotypes, and the homology was 98.41%. BLAST analysis showed that six sequences exhibited 98.09% to 98.33% similarity with the corresponding sequences of Seoul virus. The phylogenetic tree revealed that the Hantavirus agents were found in rat cluster with Seoul virus DPRK08. Conclusion Hantavirus infection was found in rodents at China-Kazakhstan border railway extension line area. The prevention and control of hantavirus in rodents should be strengthened to ensure port health safety and to prevent outbreak of the HFRS epidemic in the port area.

Key words: Small mammal, Hantavirus, Polymerase chain reaction, Alashankou pass, China-Kazakhstan border

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