中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (2): 143-146.DOI: 10.11853/j.issn.1003.8280.2018.02.007

• 论著 • 上一篇    下一篇

广东省青毛鼠的DNA条形码鉴定

姚丹丹, 冯志勇, 姜洪雪   

  1. 广东省农业科学院植物保护研究所, 植物保护新技术重点实验室, 广州 510640
  • 收稿日期:2017-09-25 出版日期:2018-04-20 发布日期:2018-04-20
  • 通讯作者: 冯志勇,Email:13318854585@163.com
  • 作者简介:姚丹丹,女,助理研究员,从事鼠类生态与防控技术研究,Email:gx-002@163.com
  • 基金资助:
    国家自然科学基金青年基金(31501662);广东省科技计划(广东省农作物病虫害绿色防控技术研究开发中心建设);广州市科技计划(201604020034,201607010020)

Identification of Berylmys bowersi based on DNA barcoding in Guangdong province

YAO Dan-dan, FENG Zhi-yong, JIANG Hong-xue   

  1. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
  • Received:2017-09-25 Online:2018-04-20 Published:2018-04-20
  • Supported by:
    Supported by the National Natural Science Foundation of China Youth Fund(No. 31501662), Science and Technology Project of Guangdong Province of China(The Construction of Green Management R & D Center for Crop Pests in Guangdong Province), and the Science and Technology Program of Guangzhou City(No. 201604020034,201607010020)

摘要: 目的 利用DNA条形码技术对未知鼠种样本进行种类鉴定。方法 以2016年在广东省阳春市捕获的2只鼠样本为研究对象,提取其基因组DNA,采用通用引物BatL5310和R6036R扩增线粒体细胞色素C氧化酶亚基Ⅰ基因片段并进行测序,将测序结果在美国国立生物技术信息中心(NCBI)中进行BLAST比对,采用邻接法构建分子系统发育树,基于双参数模型计算遗传距离。结果 2只鼠样本的DNA条形码序列一致,为同一种鼠,与NCBI中青毛鼠的序列相似度高达99%,且与青毛鼠(JN105108.1)的遗传距离为0.004 6,低于种内遗传距离1%的阈值,因此确认未知鼠为青毛鼠。结论 DNA条形码技术是一种高效、快捷的鼠类鉴定方法,可弥补形态学鉴定缺陷。

关键词: 青毛鼠, DNA条形码, 细胞色素C氧化酶亚基Ⅰ基因

Abstract: Objective Two previously unknown rodent species were identified by DNA barcoding from Yangchun city, Guangdong province. Methods Genomic DNA was extracted from samples, and the mitochondrial cytochrome c oxidase subunitⅠ genes were amplified using universal primers (BatL5310 and R6036R), then sequenced. BLAST comparison was subsequently carried out in NCBI. The phylogenetic tree was constructed using the Neighbor-Joining method. Genetic distances were calculated based on double parameter model. Results DNA sequences from two samples were consistent, suggesting they were the same species. It showed high sequence similarities with Berylmys bowersi(99%) in GenBank. In addition, genetic distance with B. bowersi was 0.004 6, less than 1% threshold of intraspecific genetic distance. Therefore, the unknown species was B. bowersi. Conclusion DNA barcoding is a highly effective approach to rapidly identify species, which could make up the shortages of morphological taxonomy.

Key words: Berylmys bowersi, DNA barcoding, Cytochrome c oxidaseⅠgene

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