中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (2): 148-150.DOI: 10.11853/j.issn.1003.4692.2015.02.010

• 论著 • 上一篇    下一篇

青海省互助、泽库和祁连县啮齿类动物伯氏疏螺旋体调查

张琳1, 石燕2, 耿震1, 侯学霞1, 郝琴1   

  1. 1 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京102206;
    2 青海省疾病预防控制中心
  • 收稿日期:2014-11-20 出版日期:2015-04-20 发布日期:2015-04-20
  • 通讯作者: 郝琴,Email:haoqin@icdc.cn
  • 作者简介:张琳,女,博士,从事莱姆病检测技术与空间生态学研究,Email:Zhanglin@icdc.cn
  • 基金资助:

    国家“十二五”传染病重大专项(2012ZX10004219, 2013ZX10004-101)

Investigation of Borrelia burgdorferi in Glires in Huzhu, Zekog, and Qilian county, Qinghai

ZHANG Lin1, SHI Yan2, GENG Zhen1, HOU Xue-xia1, HAO Qin1   

  1. 1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
    2 Qinghai Center for Disease Control and Prevention
  • Received:2014-11-20 Online:2015-04-20 Published:2015-04-20
  • Supported by:

    Supported by the 12th Five-Year Major National Science and Technology Projects of China(No. 2012ZX10004219, 2013ZX10004-101)

摘要:

目的 调查确定青海省3县啮齿类标本中伯氏疏螺旋体带菌情况。方法 从青海省互助、泽库和祁连县共采集啮齿类动物202只, 采用巢式PCR方法检测其伯氏疏螺旋体的带菌率。结果 采用巢式PCR方法检测啮齿类动物标本202份, 阳性49份, 伯氏疏螺旋体平均阳性率为24.26%。其中互助县标本共67份, 阳性12份, 阳性率为17.91%;泽库县标本共78份, 阳性27份, 阳性率为34.62%;祁连县标本共57只, 阳性10份, 阳性率为17.54%。3个县的啮齿类动物伯氏疏螺旋体阳性率差异有统计学意义(χ2=7.40, P<0.05)。结论 青海省互助、泽库和祁连县啮齿类动物中均存在伯氏疏螺旋体感染, 应进一步对当地的媒介和人群进行调查, 从而为当地莱姆病的防控提供参考。

关键词: 啮齿类动物, 伯氏疏螺旋体, 巢式聚合酶链反应, 带菌率

Abstract:

Objective To identify the Borrelia burgdorferi in Glires in Huzhu, Zekog, and Qilian county, Qinghai province. Methods A total of 202 Glires were collected from forest in the counties, Qinghai province. All the collected samples were examined for B. burgdorferi by nested PCR. Results In total of 202 samples, 49 samples were tested positive by 24.26%. Positive rate was 17.91% (12/67) in Huzhu county, 34.62% (27/78) in Zekog county and 17.54% (10/57) in Qilian county. There was significant difference with positive rate of B. burgdorferi in Glires among these three counties(χ2=7.40, P<0.05). Conclusion Our research confirmed the existence of B. burgdorferi in Glires in three counties in Qinghai province. We suggested that the further investigation on local vectors and human infections be conducted for prevention and control of Lyme disease.

Key words: Glires, Borrelia burgdorferi, Nested polymerase chain reaction, Positive rate

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