中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (3): 249-253.DOI: 10.11853/j.issn.1003.4692.2014.03.015

• 论著 • 上一篇    下一篇

伯氏疏螺旋体5S~23S rRNA和ospA基因在浙江省鼠标本检测中的应用

姜理平1, 韦亦成2, 张磊1, 应凯满2, 占利1, 陆群英1   

  1. 1 浙江省疾病预防控制中心病原微生物所, 杭州 310051;
    2 磐安县疾病预防控制中心
  • 收稿日期:2013-12-30 出版日期:2014-06-20 发布日期:2014-06-20
  • 作者简介:姜理平, 男, 主任技师, 从事自然疫源性疾病检验研究。Email: jlp1964@126.com

Application of 5S-23S rRNA and ospA genes in PCR?based detectionof Borrelia burgdorferi in rodent samples of Zhejiang province, China

JIANG Li-ping1, WEI Yi-cheng2, ZHANG Lei1, YING Kai-man2, ZHAN Li1, LU Qun-ying1   

  1. Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China;
    2 Pan’an Center for Disease Control and Prevention
  • Received:2013-12-30 Online:2014-06-20 Published:2014-06-20

摘要:

目的 探索5S~23S rRNA和ospA基因序列在伯氏疏螺旋体检测中的应用, 了解浙江省鼠中感染伯氏疏螺旋体状况。方法 用PCR方法, 检测磐安县100只鼠标本中5S~23S rRNA和ospA基因特异片段。对检测到的阳性结果进行测序。结果 检测100只鼠标本, 其中3只鼠标本伯氏疏螺旋体的5S~23S rRNA基因片段为阳性, 5只鼠标本伯氏疏螺旋体的ospA基因片段为阳性, 与参考核酸序列基本一致。结论 利用ospA和5S~23S rRNA基因能从标本中检测到伯氏疏螺旋体。

关键词: 伯氏疏螺旋体, ospA基因, 5S~23SrRNA基因, 聚合酶链反应

Abstract:

Objective To evaluate the application of 5S-23S rRNA and outer ospA genes in PCR?based detection of Borrelia burgdorferi and to investigate the infection with B. burgdorferi in rodents of Zhejiang province, China. Methods PCR was used to amplify 5S-23S rRNA and ospA gene fragments from 100 mice collected from different areas of Pan’an county. PCR products were purified and sequenced. Results Of the 100 mice investigated, 3 were positive for 5S-23S rRNA gene of B. burgdorferi and 5 for ospA gene of B. burgdorferi. The sequences of PCR products were found with high homology to the reference genes. Conclusion B. burgdorferi can be detected from rodent samples by PCR amplification of 5S-23S rRNA and ospA genes.

Key words: Borrelia burgdorferi, ospA gene, 5S-23S rRNA gene, Polymerase chain reaction

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