中国媒介生物学及控制杂志

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致倦库蚊幼虫与具抗性蚊幼虫体内可溶性蛋白的比较研究

熊美华; 邹亚丁; 徐丽娜; 王世海   

  1. 贵州省寄生虫病研究所 贵阳550004
  • 出版日期:1998-02-20 发布日期:1998-02-20

Comparative Study on Soluble Protein in Culex fatigans Larvae and Pesticides-Resistant Larvae in Guizhou

Xiong Mei-hua; Zou Ya-ding; Xu Li-na; et al   

  1. Guizhou Provincial Institute of Parasitic Diseases
  • Online:1998-02-20 Published:1998-02-20

摘要: 目的:测定致倦库蚊幼虫、具抗性蚊幼虫可溶性蛋白的组分,并分析、评价其特异性蛋白区带。方法:对正常和具抗性的Ⅲ龄、Ⅳ龄初幼虫的可溶性蛋白提取物进行聚丙烯酰胺凝胶电泳分析。结果:正常蚊幼虫主要有12条蛋白区带,其中55KDa和50KDa为其特有;对马拉硫磷及溴氰菊酯具抗性的蚊幼虫,分别有15和17条蛋白区带,并发现66KDa蛋白为特异性蛋白分子。结论:具抗性的蚊幼虫所特有的66KDa蛋白分子可能为导致蚊幼虫对杀虫剂产生抗性的特异性蛋白。

关键词: 致倦库蚊, 抗性, SDS-聚丙烯酰胺凝胶电泳

Abstract: To determine protein component, specific protein molecular from normal larvae, pesticidesresistant larvae of Culex fatigans and evaluation of the specific insecticideresistance protein bands.Soluble proteins of the late Ⅲinstar and the early Ⅳinstar larvae of both normal and pesticidesresistant mosquito were prepared.Protein component bands were tested by SDS-PAGE.The normal larvae of Culex fatigans contained mainly 12 protein bands, the 55 and 50KDa were particular protein molecular, and insecticide-resistance larvae to Malathion and Deltamethrin present at 15 and 17 protein bands respectively.The 66KDa that stronger and clearer protein bands was recognized by resistance larvae only.It could be one of the main proteins to cause mosquito larvae resistance.