关键词: 媒介蚊虫, 聚合酶链反应-限制性片段长度多态性, 细菌, 16SrDNA, 酶切类型

Abstract: Objective Bacteria in midguts of adult Anopheles mosquitoes could partially or completely inhibit oocyst formation of Plasmodium. To provide theoretical knowledge and technical information for the study on the relationship between the vector capacity and the bacteria in two kinds of mosquitoes of An.anthropophagus and An.sinensis, quantitative and categorical differences of bacteria in their genome DNA were estimated. Methods The mosquito samples were collected from Tongbai county, Henan province, and stored in 75% ethanol at -20 ℃. Their classification was re-identified by PCR and PCR-RFLP of ITS2 gene. Quantitative difference of bacteria in mosquitoes was estimated by PCR products of partial 16S rDNA sequence of bacteria. Complete 16S rDNA sequence of bacteria in 4 mosquitoes, including three An.sinensis and one An.anthropophagus, were cloned into T-vector and digested by restrictive enzyme RsaⅠ. The digested patterns were counted and analyzed. Results The category of 23 mosquitoes was confirmed based on PCR and PCR-RFLP. It has difference among bacteria copies of different mosquito DNA but it was not related with mosquitoes species. Totally, 19 digested patterns of bacteria were found in 4 mosquitoes. One common pattern widely existed in An.sinensis. Bacteria in An.anthropophagus only showed two patterns, different from that of An.sinensis. Conclusion Different kinds of bacteria located in An.anthropophagus and An.sinensis. This study supplied useful knowledge and helpful techniques to our further research in the influence of bacteria in midgut of An.sinensis and An.anthropophagus on natural transmission of malaria.