关键词: 自动液体处理器, 细菌传代, 交叉污染, 菌种保藏

Abstract:

Objective To establish high throughput semi-automated bacteria passage system. Methods Perkin Elmer automated workstation, 2-D barcode top screw frozen tubes, and disposable tips were used to establish semi-automated bacteria passage system. The recovery rate was determined and the cross-contamination of the system was compared with different parameters. Results The recovery rate depended on the times of mix and the volume of broth media. The cross-contamination was related to the volume of mixture, motion speed of the plunger and the position relationship between the disposable tips and liquid surface. Compared with manual method, this semi-automated system greatly improved the efficiency. Conclusion A high throughput semi-automated bacteria passage system was established. This system could be integrated into the routine workflow of professional bacteria culture collection lab.

Key words: Liquid automated workstation, Bacteria passage, Cross-contamination, Bacteria culture collection