Chinese Journal of Vector Biology and Control ›› 2023, Vol. 34 ›› Issue (6): 719-722.DOI: 10.11853/j.issn.1003.8280.2023.06.002

• Experimental Study • Previous Articles     Next Articles

Establishment of baseline sensitivity of laboratory strains of houseflies to commonly used insecticides

ZHANG Ya-jun1, ZHAO Chun-chun1, FENG Xing-ming2, WANG Jia-lin1, LUN Xin-chang1, LIU Qi-yong1, MENG Feng-xia1   

  1. 1. National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, WHO Collaborating Centre for Vector Surveillance and Management, Department of Vector Biology and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China;
    2. Yunnan Institute of Endemic Diseases Control and Prevention, Dali, Yunnan 671000, China
  • Received:2023-06-29 Online:2023-12-20 Published:2023-12-26
  • Supported by:
    Vector Surveillance and Control Project (No. 102393220020020000012); National Science and Technology Major Project of China (No. 2018ZX10101002-002)


张亚军1, 赵春春1, 冯星明2, 王佳琳1, 伦辛畅1, 刘起勇1, 孟凤霞1   

  1. 1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病溯源预警与智能决策全国重点实验室, WHO媒介生物监测与管理合作中心, 北京 102206;
    2. 云南省地方病防治所, 云南 大理 671000
  • 通讯作者: 孟凤霞,
  • 作者简介:张亚军,女,在读硕士,主要从事媒介生物抗药性研究,
  • 基金资助:

Abstract: Objective To establish the sensitive baseline of houseflies to six commonly used insecticides, so as to provide a basis for the monitoring and control of the insecticide resistance of houseflies.Methods The topical application method was used to test the sensitivity of laboratory susceptible housefly strains to six insecticides (deltamethrin, beta-cypermethrin, permethrin, dichlorvos, propoxur, and dinotefuran) of four categories. Data from previous literature were collected for comparison. The statistical significance of sensitivity differences was determined according to whether the 95% confidence interval of the median lethal dose (LD50) overlapped. Based on the above steps, the baseline sensitivity of houseflies to the six insecticides was established.Results The baseline sensitivity of the six test insecticides against houseflies were as follows: deltamethrin (LD50=0.002 236 μg/♀, y=13.76+5.19x), beta-cypermethrin (LD50=0.003 692 μg/♀, y=9.34+3.84x), permethrin (LD50=0.007 152 μg/♀, y=10.03+4.67x), dichlorvos (LD50=0.074 20 μg/♀, y=5.02+4.44x), propoxur (LD50=0.415 9 μg/♀, y=0.93+2.43x), and dinotefuran (LD50=0.103 6 μg/♀, y=6.31+6.41x).Conclusion This study established the baseline sensitivity of houseflies through testing the sensitivity of laboratory susceptible strains to six commonly used insecticides, providing a reference for monitoring and assessing the resistance level of houseflies in China.

Key words: Housefly, Topical application, Insecticide, Baseline sensitivity

摘要: 目的 该研究旨在建立家蝇对6种常用杀虫剂的敏感基线,为我国家蝇抗药性系统监测和防控提供依据。方法 利用点滴法测定本实验室常年饲养的家蝇敏感品系对4大类共6种杀虫剂(溴氰菊酯、高效氯氰菊酯、氯菊酯、敌敌畏、残杀威、呋虫胺)的敏感性,同时收集历史文献中的数据,以半数致死剂量(LD50)的95%置信区间(95%CI)是否重叠来判断敏感性差异有无统计学意义,基于以上方法建立家蝇对这6种杀虫剂的敏感基线。结果 供试的6种杀虫剂对家蝇的敏感基线依次为:溴氰菊酯(LD50=0.002 236 μg/♀,y=13.76+5.19x)、高效氯氰菊酯(LD50=0.003 692 μg/♀,y=9.34+3.84x)、氯菊酯(LD50=0.007 152 μg/♀,y=10.03+4.67x)、敌敌畏(LD50=0.074 20 μg/♀,y=5.02+4.44x)、残杀威(LD50=0.415 9 μg/♀,y=0.93+2.43x)、呋虫胺(LD50=0.103 6 μg/♀,y=6.31+6.41x)。结论 测定了实验室敏感品系家蝇对6种常用杀虫剂的敏感水平,建立了敏感基线,为我国家蝇抗药性监测和抗性水平评估提供了参考依据。

关键词: 家蝇, 点滴法, 杀虫剂, 敏感基线

CLC Number: