Insecticide resistance and metabolic enzyme activity monitoring for Aedes albopictus in Tongren of Guizhou province, China

doi:10.11853/j.issn.1003.8280.2022.06.004

Abstract

Abstract: Objective To investigate the resistance level of Aedes albopictus to commonly used insecticides in Tongren of Guizhou province, China and the mechanism of metabolic resistance at the enzyme level, and to provide a theoretical basis for the control and resistance management of Ae. albopictus. Methods In August 2020, Ae. albopictus larvae were collected from small water containers in communities at different locations of Tongren and were reared to F1-F2 generations. The larval dipping method and the adult mosquito contact tube method were used to investigate the insecticide resistance of the larvae and adult mosquitoes of Ae. albopictus, and a microplate reader was used to measure the activity of non-specific esterase (NSE), mixed function oxidase (MFO), and glutathione-S-transferase (GST). SPSS 24.0 software was used to perform the statistical analysis of laboratory results, and Minitab 20 software was used to make charts. Results For the larvae of the field population of Ae. albopictus, the resistance ratios to deltamethrin, beta-cypermethrin, permethrin, temephos, and propoxur were 15.38, 7.88, 61.44, 1.70, and 1.90 folds, respectively. The 24 h mortality rates of adult mosquitoes due to deltamethrin, beta-cypermethrin, permethrin, malathion, and propoxur at diagnostic doses were 0, 58.00%, 2.13%, 79.25%, and 100%, respectively. The activities of NSE, MFO, and GST in the field population of Ae. albopictus were 1.21, 0.28, and 0.34 times, respectively, those in sensitive strains, and the activities of NSE, MFO, and GST in the field population showed a positively skewed distribution. Conclusion The Ae. albopictus larvae in Tongren have developed varying degrees of resistance to pyrethroid insecticides and remain sensitive to temephos or propoxur, while the adult mosquitoes are resistant to pyrethroid insecticides and malathion and sensitive to propoxur. Therefore, it is suggested to adopt comprehensive measures for the control of Ae. albopictus and use chemical insecticides scientifically and rationally, so as to effectively control the development and progression of insecticide resistance in Ae. albopictus.

Key words: Aedes albopictus, Insecticide, Insecticide resistance, Metabolic enzyme

摘要： 目的掌握贵州省铜仁市白纹伊蚊对常用杀虫剂的抗药性水平，并从酶学水平初步探索其代谢抗性机制，为当地白纹伊蚊控制及抗药性治理提供理论依据。方法 2020年8月在铜仁市不同方位居民区的小型积水容器中采集白纹伊蚊幼蚊，经饲养室繁殖至F1～F2代，采用幼虫浸渍法及成蚊接触筒法对当地白纹伊蚊幼蚊及成蚊进行抗药性监测，并采用酶标仪检测幼蚊非特异性酯酶（NSE）、多功能氧化酶（MFO）和谷胱甘肽-S-转移酶（GST）的活力，实验室结果所得数据采用SPSS 24.0软件进行统计学分析，Minitab 20软件制图。结果白纹伊蚊野外种群幼蚊对溴氰菊酯、高效氯氰菊酯、氯菊酯、双硫磷和残杀威的抗性倍数分别为15.38、7.88、61.44、1.70和1.90倍。成蚊对溴氰菊酯、高效氯氰菊酯、氯菊酯、马拉硫磷、残杀威诊断剂量的24 h死亡率分别为0、58.00%、2.13%、79.25%和100%。白纹伊蚊野外种群NSE、MFO、GST活力是敏感品系的1.21、0.28和0.34倍。野外种群NSE、MFO及GST活力均呈正偏态分布。结论铜仁市白纹伊蚊幼蚊对拟除虫菊酯类杀虫剂均已产生不同程度的抗药性，对双硫磷或残杀威敏感，而成蚊对3种拟除虫菊酯类杀虫剂和马拉硫磷呈现抗性，对残杀威敏感。建议今后当地白纹伊蚊防治工作遵循综合防制原则，科学合理的选择化学杀虫剂，以便有效控制抗药性的发生及发展。

关键词: 白纹伊蚊, 杀虫剂, 抗药性, 代谢酶

CLC Number:

WANG Dan, CHEN Dan-dan, TIAN Dong-dong, XU Xiu-ping, ZHOU Jing-zhu, SHI Wei-fang, LIANG Wen-qin. Insecticide resistance and metabolic enzyme activity monitoring for Aedes albopictus in Tongren of Guizhou province, China[J]. Chinese Journal of Vector Biology and Control, 2022, 33(6): 787-792.

王丹, 陈丹丹, 田冬冬, 徐秀平, 周敬祝, 师伟芳, 梁文琴. 贵州省铜仁市白纹伊蚊抗药性及代谢酶活性监测研究[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 787-792.

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