Resistance of Musca domestica to six insecticides commonly used in Hefei, China, 2021

doi:10.11853/j.issn.1003.8280.2023.01.019

Abstract

Abstract: Objective To investigate the insecticide resistance level of Musca domestica in human settlements and surroundings in Hefei, China, and to provide a scientific basis for insecticide use and management as well as fly control. Methods The topical application method was used to determine the resistance levels of M. domestica to insecticides. Probit analysis was performed with SPSS 22.0 software to calculate the toxicity regression equation as well as median lethal dose (LD₅₀) and 95% confidence interval. Results For M. domestica in Hefei, 2021, the LD₅₀ values of dichlorvos, permethrin, deltamethrin, alpha-cypermethrin, beta-cypermethrin, and lambda-cyhalothrin were 0.321 55, 0.775 62, 0.956 54, 0.519 00, 0.542 90, and 0.532 90 μg/♀, respectively; the relative resistance coefficients were 28.21, 133.73, 1 471.60, 112.83, 63.13, and 166.53, respectively, indicating high resistance levels of M. domestica to all the six insecticides. Conclusion M. domestica in Hefei developed high resistance to the six commonly used insecticides, which should be avoided or reduced in use. Other insecticides of high sensitivity or with different mechanisms of action should be used in alternation to delay the development of resistance.

Key words: Hefei, Musca domestica, Insecticide, Insecticide resistance, Topical application method

摘要： 目的了解合肥市人居及周边环境中家蝇的抗药性水平现状，为制定科学、规范的卫生杀虫剂使用、管理策略及科学开展蝇类防制工作提供科学依据。方法成蝇抗药性测定采用点滴法。采用SPSS 22.0统计软件的Probit模块对数据进行处理，求毒力回归方程及半数致死剂量（LD₅₀）和95%置信区间。结果 2021年合肥市家蝇对敌敌畏、氯菊酯、溴氰菊酯、顺式氯氰菊酯、高效氯氰菊酯和高效氯氟氰菊酯6种杀虫剂的LD₅₀值分别为0.321 55、0.775 62、0.956 54、0.519 00、0.542 90和0.532 90 μg/雌，抗性倍数依次为28.21、133.73、1 471.60、112.83、63.13和166.53倍，对敌敌畏和5种菊酯类均属于高度抗性水平。结论合肥市家蝇对6种常用卫生杀虫剂均已产生高度抗药性，应停止或减少这6种杀虫剂的使用，寻找和轮换使用其他作用机制或敏感型的卫生杀虫剂，以期延缓抗药性的发生和发展速度。

关键词: 合肥市, 家蝇, 杀虫剂, 抗药性, 点滴法

CLC Number:

R384.2

Yin-xu HOU, Zhi-wei LIU, Yan-jie ZHANG, Feng-tao YANG, Wen-jun WANG, Feng-xia MENG, Fa-yuan HUANG. Resistance of Musca domestica to six insecticides commonly used in Hefei, China, 2021[J]. Chinese Journal of Vector Biology and Control, 2023, 34(1): 105-108.

侯银续, 刘志伟, 张燕婕, 仰凤桃, 王文君, 孟凤霞, 黄发源. 2021年合肥市家蝇对6种常用卫生杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2023, 34(1): 105-108.

References

[1] 王陇德. 病媒生物防制实用指南[M]. 北京:人民卫生出版社, 2010:61-74, 267. Wang LD. Vector control (practical guide)[M]. Beijing:People's Medical Publishing House, 2010:61-74, 267. (in Chinese)
[2] 杨晗晗. 中山市不同生境蝇类分布及常见蝇类携带细菌的比较研究[D]. 广州:广东药科大学, 2019. Yang HH. Comparative study on the distribution of flies in different habitats and comparative study on bacteria carried by common flies of Zhongshan[D]. Guangzhou:Guangdong Pharmaceutical University, 2019. (in Chinese)
[3] 姜陆. 家蝇抗性机制研究进展[J]. 口岸卫生控制, 2015, 20(3):56-60. DOI:10.3969/j.issn.1008-5777.2015.03.017. Jiang L. Progress of resistance mechanisms study of the housefly[J]. Port Health Control, 2015, 20(3):56-60. DOI:10.3969/j.issn.1008-5777.2015.03.017.(in Chinese)
[4] 向玉勇, 程凯, 周书芹, 等. 滁州市家蝇对6种杀虫剂的抗药性调查[J]. 四川动物, 2012, 31(1):113-115. DOI:10.3969/j.issn.1000-7083.2012.01.026. Xiang YY, Cheng K, Zhou SQ, et al. Investigation of the insecticide resistance of Musca domestica to 6 pesticides in Chuzhou[J]. Sichuan J Zool, 2012, 31(1):113-115. DOI:10.3969/j.issn.1000-7083.2012.01.026.(in Chinese)
[5] 刘艳, 李梅, 邱星辉. 家蝇对氯菊酯抗性的分子机制研究[J]. 寄生虫与医学昆虫学报, 2020, 27(2):106-111. DOI:10.3969/j.issn.1005-0507.2020.02.007. Liu Y, Li M, Qiu XH. Pinpointing the molecular mechanisms of permethrin resistance in the housefly Musca domestica[J]. Acta Parasitol Med Entomol Sin, 2020, 27(2):106-111. DOI:10.3969/j.issn.1005-0507.2020.02.007.(in Chinese)
[6] 魏尊. 2021年咸宁市家蝇和白纹伊蚊的抗药性监测[J]. 中华卫生杀虫药械, 2022, 28(3):205-207. DOI:10.19821/j.1671-2781.2022.03.004. Wei Z. Resistance of Musca domestica and Aedes albopictus in Xianning in 2021[J]. Chin J Hyg Insect Equip, 2022, 28(3):205-207. DOI:10.19821/j.1671-2781.2022.03.004.(in Chinese)
[7] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB/T 26350-2010蝇类抗药性检测方法家蝇生物测定法[S]. 北京:中国标准出版社, 2011. Inspection and Quarantine of the People's Republic of China, Standardization Administration of China. GB/T 26350-2010 Test methods of fly resistance to insecticides-Bioassay methods for Musca domestica[S]. Beijing:Standards Press of China, 2011. (in Chinese)
[8] 施红喜, 何健, 李培珍. 金华市不同生境家蝇抗药性分析[J]. 预防医学, 2019, 31(9):906-907, 910. DOI:10.19485/j.cnki.issn2096-5087.2019.09.010. Shi HX, He J, Li PZ. Resistance of Musca domestica in different habitats in Jinhua[J]. Prev Med, 2019, 31(9):906-907, 910. DOI:10.19485/j.cnki.issn2096-5087.2019.09.010.(in Chinese)
[9] 任志华, 刘天, 潘引君. 上海市青浦区家蝇对常用杀虫剂的抗性调查[J]. 中华卫生杀虫药械, 2018, 24(6):547-548. DOI:10.19821/j.1671-2781.2018.06.010. Ren ZH, Liu T, Pan YJ. Resistance investigation of Musca domestica to commonly used insecticides in Qingpu district of Shanghai city[J]. Chin J Hyg Insect Equip, 2018, 24(6):547-548. DOI:10.19821/j.1671-2781.2018.06.010.(in Chinese)
[10] 王唐, 宋灿磊, 李淑华, 等. 上海市金山区家蝇对5种杀虫剂的抗性调查[J]. 中华卫生杀虫药械, 2021, 27(4):382-383. DOI:10.19821/j.1671-2781.2021.04.024. Wang T, Song CL, Li SH, et al. Resistance of Musca domestica to five insecticides in Jinshan district of Shanghai[J]. Chin J Hyg Insect Equip, 2021, 27(4):382-383. DOI:10.19821/j.1671-2781.2021.04.024.(in Chinese)
[11] 马玉涛. 天津市津南区家蝇对常用卫生杀虫剂的抗药性研究[J]. 中国媒介生物学及控制杂志, 2019, 30(2):221-223. DOI:10.11853/j.issn.1003.8280.2019.02.026. Ma YT. Resistance of Musca domestica to common hygienic insecticides in Jinnan district, Tianjin, China[J]. Chin J Vector Biol Control, 2019, 30(2):221-223. DOI:10.11853/j.issn.1003. 8280.2019.02.026.(in Chinese)
[12] 胡兴强, 吴明生, 李荣民, 等. 合肥市屠宰场家蝇对10种杀虫剂的抗药性研究[J]. 中国媒介生物学及控制杂志, 2001, 12(5):350-352. DOI:10.3969/j.issn.1003-4692.2001.05.010. Hu XQ, Wu MS, Li RM, et al. Study on the resistance against ten insecticides of house fly in slaughterhouse of Hefei[J]. Chin J Vector Biol Control, 2001, 12(5):350-352. DOI:10.3969/j.issn.1003-4692.2001.05.010.(in Chinese)
[13] 周健, 胡兴强. 2004年合肥市家蝇抗药性调查[J]. 中华卫生杀虫药械, 2005, 11(4):258-259. DOI:10.3969/j.issn.1671-2781.2005.04.015. Zhou J, Hu XQ. Survey on resistance to insecticides of Musca domestica in Hefei[J]. Chin J Hyg Insect Equip, 2005, 11(4):258-259. DOI:10.3969/j.issn.1671-2781.2005.04.015.(in Chinese)
[14] 胡兴强, 吴明生, 李荣民. 家蝇对3种杀虫剂的抗性调查[J]. 安徽预防医学杂志, 1996, 2(2):23-24. Hu XQ, Wu MS, Li RM. The investigation of house fly resistivity to three pesticides in Hefei[J]. Anhui J Prev Med, 1996, 2(2):23-24. (in Chinese)
[15] 胡文涛. 家蝇对高效氯氰菊酯抗药性机制的探索[J]. 中华卫生杀虫药械, 2008, 14(2):87-91. DOI:10.3969/j.issn.1671-2781.2008.02.004. Hu WT. Resistance mechanisms of Musca domestica against beta-cypermethrin[J]. Chin J Hyg Insect Equip, 2008, 14(2):87-91. DOI:10.3969/j.issn.1671-2781.2008.02.004.(in Chinese)
[16] 陈红娜, 褚宏亮, 刘大鹏, 等. 家蝇对常用杀虫剂的抗药性调查[J]. 江苏预防医学, 2015, 26(3):98-99. DOI:10.13668/j.issn.1006-9070.2015.03.040. Chen HN, Chu HL, Liu DP, et al. Investigation of resistance of Musca domestica to commonly used insecticides[J]. Jiangsu J Prev Med, 2015, 26(3):98-99. DOI:10.13668/j.issn.1006-9070.2015.03.040.(in Chinese)
[17] 韦凌娅, 孔庆鑫, 曹阳, 等. 杭州市家蝇高效氯氰菊酯抗性品系的建立[J]. 中国媒介生物学及控制杂志, 2022, 33(4):458-461. DOI:10.11853/j.issn.1003.8280.2022.04.002. Wei LY, Kong QX, Cao Y, et al. Establishment of beta-cypermethrin-resistant strains of Musca domestica in Hangzhou[J]. Chin J Vector Biol Control, 2022, 33(4):458-461. DOI:10.11853/j.issn.1003.8280.2022.04.002.(in Chinese)
[18] 侯银续, 陈李, 钱帮群, 等. 安徽省芜湖市登革热媒介白纹伊蚊的抗药性研究[J]. 中国媒介生物学及控制杂志, 2021, 32(1):78-80, 88. DOI:10.11853/j.issn.1003.8280.2021.01.016. Hou YX, Chen L, Qian BQ, et al. An investigation of insecticide resistance of the dengue vector Aedes albopictus in Wuhu city of Anhui province, China[J]. Chin J Vector Biol Control, 2021, 32(1):78-80, 88. DOI:10.11853/j.issn.1003.8280.2021.01.016.(in Chinese)