An analysis of resistance of Blattella germanica to commonly used insecticides in Shenzhen, China
HU Jing1, LIU Yang2, ZHANG Shao-hua2, QIN Yan-min2, LIANG Zhuo-nan2
1. School of Public Health, Nanhua University, Hengyang, Hunan 425000, China; 2. Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, China
Abstract：Objective To investigate the resistance of Blattella germanica to commonly used insecticides in ten districts of Shenzhen, China and the dynamic development rules of such insecticide resistance, and to provide a scientific basis for the drug control of cockroaches and the formulation of effective prevention and control programs. Methods According to the residual film method in Cockroach insecticide resistance monitoring program (On trial) formulated by National Study Group of Cockroach Prevention and Control, 2.5 ml of 0.05% insecticide solution was placed into a 500 ml transparent wide-mouth bottle, and the wide-mouth bottle was rolled to form a uniform drug membrane. The male adults of B. germanica were put into the bottle, and the knockdown of the test insects was observed and recorded at a certain interval. SPSS 24.0 software was used to calculate median knockdown time. Results Beta-cypermethrin showed high resistance in Longgang district, with a resistance ratio of 11.34, medium resistance in Bao'an, Guangming, and Futian districts, with resistance ratios of 8.79, 8.77, and 8.07, respectively, and low resistance in the other districts. Permethrin showed high resistance in Futian, Longhua, Bao'an, Longgang, and Luohu districts, with resistance ratios of 22.26, 13.99, 14.28, 13.01, and 10.01, respectively, medium resistance in Pingshan district, with a resistance ratio of 5.51, and low resistance in the other districts. Deltamethrin showed medium resistance in Bao'an, Longgang, and Longhua districts, with resistance ratios of 7.00, 6.39, and 6.02, respectively, and low resistance in the other districts. Cypermethrin showed high resistance in Bao'an district, with a resistance ratio of 19.65, medium resistance in Futian district, with a resistance ratio of 9.42, and low resistance in the other districts. Chlorpyrifos, acephate, and propoxur showed low resistance in all districts. Conclusion Varying degrees of resistance to pyrethroid insecticides are observed in B. germanica in Shenzhen. It is suggested to stop or restrict the use of insecticides with medium and high resistance and to use chlorpyrifos, acephate, and propoxur with low resistance. At the same time, comprehensive control measures should be taken to control and delay the development and progression of insecticide resistance in B. germanica.
胡静, 刘阳, 张韶华, 秦彦珉, 梁焯南. 广东省深圳市德国小蠊对常用杀虫剂的抗药性分析[J]. 中国媒介生物学及控制杂志, 2021, 32(1): 70-73.
HU Jing, LIU Yang, ZHANG Shao-hua, QIN Yan-min, LIANG Zhuo-nan. An analysis of resistance of Blattella germanica to commonly used insecticides in Shenzhen, China. Chines Journal of Vector Biology and Control, 2021, 32(1): 70-73.
[1] Haverinen-Shaughnessy U,Khan S,Boulafentis J,et al. Effects of educational efforts in tribal homes and schools to reduce asthma triggers,symptoms and missed school days[J]. Int J Hyg Environ Health,2020,228:113513. DOI:10.1016/j.ijheh.2020.113513. [2] 林良强,张韶华,张燕娜,等. 深圳市病媒生物监测分析[J]. 中华卫生杀虫药械,2018,24(2):154-157. DOI:10.19821/j.1671-2781.2018.02.012.Lin LQ,Zhang SH,Zhang YN,et al. Survey on main medical vectors in Shenzhen city[J]. Chin J Hyg Insect Equip,2018,24(2):154-157. DOI:10.19821/j.1671-2781.2018.02.012. [3] 孙俊,褚宏亮,杨维芳,等. 我国蟑螂抗药性现状与检测方法的研究[J]. 中华卫生杀虫药械,2011,17(1):12-17. DOI:10.19821/j.1671-2781.2011.01.005.Sun J,Chu HL,Yang WF,et al. Current situation and testing method of cockroach resistance in China[J]. Chin J Hyg Insect Equip,2011,17(1):12-17. DOI:10.19821/j.1671-2781.2011. 01.005. [4] 李海龙,张桂林,党荣理,等. 应用SPSS软件进行杀虫剂的KT50分析[J]. 中国媒介生物学及控制杂志,2011,22(2):155-157.Li HL,Zhang GL,Dang RL,et al. Calculation of KT50 of pesticides with SPSS software[J]. Chin J Vector Biol Control,2011,22(2):155-157. [5] 明明. 泰安市蟑螂密度季节消长及其抗药性分析[J]. 现代预防医学,2016,43(19):3476-3478.Ming M. Seasonal fluctuation of cockroach density and its resistance,Tai'an city[J]. Mod Prev Med,2016,43(19):3476-3478. [6] 张守刚,熊丽林,孙燕群,等. 南京市蟑螂密度监测及抗药性分析[J]. 中华卫生杀虫药械,2018,24(1):20-23. DOI:10.19821/j.1671-2781.2018.01.007.Zhang SG,Xiong LL,Sun YQ,et al. Study on the density and insecticide resistance of cockroaches in Nanjing city[J]. Chin J Hyg Insect Equip,2018,24(1):20-23. DOI:10.19821/j.1671-2781.2018.01.007. [7] 龙再浩,俞雪均,倪敏君. 宁波口岸德国小蠊对6种杀虫剂的抗药性调查[J]. 中华卫生杀虫药械,2017,23(1):88-89. DOI:10.19821/j.1671-2781.2017.01.027.Long ZH,Yu XJ,Ni MJ. Resistance of Blattella germanica to six kinds of insecticides in Ningbo port[J]. Chin J Hyg Insect Equip,2017,23(1):88-89. DOI:10.19821/j.1671-2781.2017.01.027. [8] 徐正龙,葛伟,朱红艳. 2014年张家港市德国小蠊的抗药性调查[J]. 中华卫生杀虫药械,2015,21(5):477-478. DOI:10.19821/j.1671-2781.2015.05.012.Xu ZL,Ge W,Zhu HY. Investigation on the resistance of Blattella germanica to insecticides in Zhangjiagang city in 2014[J]. Chin J Hyg Insect Equip,2015,21(5):477-478. DOI:10.19821/j.1671-2781.2015.05.012. [9] 苏庆盛,罗林峰,林琳,等. 深圳市德国小蠊和家蝇对常用杀虫剂的抗性研究[J]. 现代预防医学,2014,41(4):698-699,703.Su QS,Luo LF,Lin L,et al. Resistance of Blattella germanica and Musca domestica to commonly-used insecticides in Shenzhen city[J]. Mod Prev Med,2014,41(4):698-699,703. [10] 钟积成,杨闳智,朱礼平,等. 深圳市大鹏新区德国小蠊抗药性及与抗药性相关的几种酶活性关系的研究[J]. 现代预防医学,2019,46(9):1680-1683.Zhong JC,Yang HZ,Zhu LP,et al. Chemical resistance and several correlated enzyme activities of the Blattella germanica in Dapeng new district,Shenzhen[J]. Mod Prev Med,2019,46(9):1680-1683.
2021, Vol. 32 
