Abstract:Objective To study the cross-immune responses and epidemiological significance of three pathogenic Yersinia species to host rats. Methods The host animals of plague in Guangdong province, Rattus losea, R. tanezumi, and R. norvegicus, were immunized four times (2×109, 3×109, 6×109, and 7×109 cells/rat, respectively) with two strains of live Y. pseudotuberculosis and three strains of live Y. enterocolitica, respectively, and the control group of rats were set at the same time. Then the appropriate amount of Y. pestis EV76 strain solution mixed with 0.3 ml of 0.30% ferric ammonium citrate solution was used for a challenge test to evaluate the presence or absence of cross-immune protection. The rates were compared by using χ2 test or Fisher's exact test. ResultsRattus losea:Y. pestis EV76 strain was cultured at 28℃ and given at 2.5×109 cells/rat in the challenge test; the mortality rate was 9.09% (2/22) for the Y. pseudotuberculosis immunization group, 31.25% (10/32) for the Y. enterocolitica immunization group, and 68.75% (22/32) for the control group, with significant differences among the Y. pseudotuberculosis immunization group, Y. enterocolitica immunization group, and the control group (χ2=18.793, P<0.001; χ2=9.000, P=0.003), but there was no significant difference between the Y. pseudotuberculosis immunization group and the Y. enterocolitica immunization group (χ2=2.533, P=0.112). Rattus tanezumi:Y. pestis EV76 strain was cultured at 37℃ and given at 3.8×109 cells/rat in the challenge test; the mortality rate was significantly lower in the Y. pseudotuberculosis immunization group than in the control group[18.18% (4/22) vs 91.30% (21/23), χ2=24.350, P<0.001]; there was no significant difference in mortality rate between the Y. enterocolitica immunization group and the control group[90.91% (30/33) vs 91.30% (21/23), χ2=0.000, P=1.000]; there was a significant difference in mortality rate between the Y. pseudotuberculosis immunization group and the Y. enterocolitica immunization group (χ2=29.580, P<0.001). Another group of R. tanezumi:Y. pestis EV76 strain was cultured at 37℃ and given at 3×109 cells/rat in the challenge test; the mortality rate was significantly different between the Y. enterocolitica immunization group and the control group[3.45% (1/29) vs 45.45% (5/11), P=0.004]. Rattus norvegicus:Y. pestis EV76 strain was cultured at 37℃ and given at 3.5×109 cells/rat in the challenge test; the mortality rate was significantly different between the Y. enterocolitica immunization group and the control group[3.70% (1/27) vs 61.11% (11/18), χ2=15.384,P<0.001]. Conclusion With effective immunity, Y. pseudotuberculosis can provide strong cross-immune protection against Y. pestis in host rats, and Y. enterocolitica can also provide a certain cross-immune protection.
潘珠, 梁秋光, 岑清泉, 黄济英, 陈冠森, 杨柳, 杨华源. 3种致病性耶尔森菌对宿主鼠的交叉免疫反应研究[J]. 中国媒介生物学及控制杂志, 2020, 31(5): 540-544.
PAN Zhu, LIANG Qiu-guang, CEN Qing-quan, HUANG Ji-ying, CHEN Guan-sen, YANG Liu, YANG Hua-yuan. Study on cross-immune responses of three pathogenic Yersinia species to host rats. Chines Journal of Vector Biology and Control, 2020, 31(5): 540-544.
[1] 陈妤煌. 致病性耶尔森菌外膜蛋白A同源性分析及免疫学特征研究[D]. 北京:中国疾病预防控制中心,2016. Chen YH. Homology analysis and immunological characters study of OmpA from pathogenic Yersinia[D]. Beijing:Chinese Center for Disease Control and Prevention,2016. [2] 杨华源,刘小华,张曼碧,等. 鼠疫菌EV株免疫球蛋白反向血凝试验的敏感性和特异性试验[J]. 热带医学杂志,2005,5(5):614-616. Yang HY,Liu XH,Zhang MB,et al. Sensitivity and specificity of the detection of immunoglobulin of Yersinia pestis EV strsin by RIHA test[J]. J Trop Med,2005,5(5):614-616. [3] 汪华,景怀琦,朱凤才,等. 小肠结肠炎耶尔森菌[M]. 北京:人民出版社,2004:4. Wang H,Jing HQ,Zhu FC,et al. Yersinia enterocolitica[M]. Beijing:People Publishing House,2004:4. [4] 申小娜,海荣,俞东征. 鼠疫菌基因组学研究进展[J]. 疾病监测,2009,24(6):440-445. DOI:10.3784/j.issn.1003-9961.2009.06.019. Shen XN,Hai R,Yu DZ. Developments in genomic study of Yersinia pestis[J]. Dis Surveil,2009,24(6):440-445. DOI:10.3784/j.issn.1003-9961.2009.06.019. [5] 谷爱娣,樊振亚. 3种对人致病性耶尔森氏菌生物学特性及流行病学意义[J]. 中国地方病防治杂志,2001,16(4):218-222. DOI:10.3969/j.issn.1001-1889.2001.04.011. Gu AD,Fan ZY. The biological characteristics and epidemiological significance of three pathogenic Yersinia[J]. Chin J Control Endem Dis,2001,16(4):218-222. DOI:10.3969/j.issn.1001-1889.2001.04.011. [6] 张孝和,陈祎,倪庆翔,等. 浙江省温州市鼠疫静息期鼠类携带耶尔森菌调查分析[J]. 疾病监测,2010,25(9):744-745. DOI:10.3784/j.issn.1003-9961.2010.09.024. Zhang XH,Chen Y,Ni QX,et al. Survey on the carriage of Yersinia in rodents in silent period of plague in Wenzhou[J]. Dis Surveil,2010,25(9):744-745. DOI:10.3784/j.issn.1003-9961.2010.09.024. [7] 陈双艳,郭英,张海鹏,等. 2013-2014年云南省剑川县野鼠鼠疫疫源地小肠结肠炎耶尔森菌的调查分析[J]. 疾病监测,2016,31(2):141-144. DOI:10.3784/j.issn.1003-9961.2016.02.013. Chen SY,Guo Y,Zhang HP,et al. Investigation of Yersinia enterocolitica in wild plague foci in Jianchuan,Yunnan[J]. Dis Surveil,2016,31(2):141-144. DOI:10.3784/j.issn.1003-9961.2016.02.013. [8] 段然,梁俊容,张婧,等. 我国西南3省交界处静息鼠疫疫源地致病性耶尔森菌研究[J]. 中国媒介生物学及控制杂志,2017,28(3):241-243,261. DOI:10.11853/j.issn.1003.8280.2017.03.011. Duan R,Liang JR,Zhang J,et al. Research on pathogenic Yersinia within natural plague focus during rest periods on the border of three provinces in Southwest of China[J]. Chin J Vector Biol Control,2017,28(3):241-243,261. DOI:10.11853/j.issn.1003.8280.2017.03.011. [9] 沈荣煊,邓峰,刘铭泉,等. 广东鼠疫[M]. 广州:广东科技出版社,2005:110-119. Shen RX,Deng F,Liu MQ,et al. Guangdong plague[M]. Guangzhou:Guangdong Science & Technology Press,2005:110-119. [10] 刘小华,杨华源,梁秋光,等. 湛江市人和动物假结核病血清学调查[J]. 中国人兽共患病杂志,1997,13(5):71. Liu XH,Yang HY,Liang QG,et al. Serological investigation of human and animal pseudotuberculosis in Zhanjiang city[J]. Chin J Zoonoses,1997,13(5):71. [11] 杨华源,梁秋光,曾敏,等. 广东鼠疫静息期小肠结肠炎、假结核耶尔森氏菌流行病学调查[J]. 中国地方病防治杂志,2009,24(5):341-342. Yang HY,Liang QG,Zeng M,et al. Epidemiological survey on Yersinia enterocolitica and Y. pseudotubercolosis of plague static phase in Guangdong[J]. Chin J Control Endem Dis,2009,24(5):341-342. [12] Mazigh D,Quilici ML,Mollaret HH. Role of the virulence-associated plasmids of Yersinia enterocolitica on its immunogenicity against Y. pestis[J]. Ann Microbiol,1984,135(3):283-290.