中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (3): 294-298.DOI: 10.11853/j.issn.1003.4692.2015.03.019

• 论著 • 上一篇    下一篇

黑龙江省黑瞎子岛地区小型哺乳动物的调查研究

王彩乔1, 李明2, 郭文平3, 富英群2, 王文3, 曲立民4, 李明慧3, 潘光东4, 田喜凤1   

  1. 1 河北联合大学基础医学院, 河北唐山063000;
    2 黑龙江出入境检验检疫局, 哈尔滨150000;
    3 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室;
    4 抚远出入境检验检疫局
  • 收稿日期:2014-12-29 出版日期:2015-06-20 发布日期:2015-06-20
  • 通讯作者: 田喜凤,Email: tstianxifeng@163.com
  • 作者简介:王彩乔,女,在读硕士,从事病毒的分子流行病学调查及其宿主鉴定,Email: 790036721@qq.com;李明,男,从事标本的采集工作,Email: 297553262@qq.com

Survey on small mammals on the Bolshoy Ussuriysky Island of Heilongjiang province, China

WANG Cai-qiao1, LI Ming2, GUO Wen-ping3, FU Ying-qun2, WANG Wen3, QU Li-min4, LI Ming-hui3, PAN Guang-dong4, TIAN Xi-feng1   

  1. 1 College of Elementary Medicine, Hebei United University, Tangshan 063000, Hebei Province, China;
    2 Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin 150000, Heilongjiang Province, China;
    3 State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention;
    4 Fuyuan Entry-Exit Inspection and Quarantine Bureau
  • Received:2014-12-29 Online:2015-06-20 Published:2015-06-20

摘要:

目的 了解黑龙江省黑瞎子岛地区小型哺乳动物的物种多样性及其与其他地区相应物种间的关系,为可能存在于该岛的自然疫源性疾病的病原体筛查工作奠定基础,为疾病的预防与控制提供科学依据。方法 用夹夜法捕捉小型哺乳动物,首先进行形态学鉴定,然后用PCR方法扩增线粒体细胞色素b(mt-cyt b)基因并测序,所得序列与GenBank中该物种的不同地区相应序列进行同源性比较和系统发育分析。结果 共捕获374只小型哺乳动物,分属于2目4科6属9种,其中黑线姬鼠和红背鼠平为优势鼠种。形态学和分子生物学方法鉴定结果相一致。结论 黑瞎子岛上小型哺乳动物物种复杂,具有多样性,并且mt-cyt b基因未发生明显变异。岛上多个物种可以携带自然疫源性疾病的病原体,因此该研究对当地的疾病预防与控制具有重要的指导意义。

关键词: 黑瞎子岛, 小型哺乳动物, 系统发育分析

Abstract:

Objective To investigate the species diversity of small mammals and their phylogenetic relationship in the Chinese part of the Bolshoy Ussuriysky Island, to facilitate pathogen screening of natural focal disease existing in the island and to provide scientific basis for disease prevention and control. Methods Small mammals were trapped using baited cages set in the evening and retrieved in the morning after a trapping night. Firstly, these mammals were identified using morphological characteristics. Secondly, mitochondrial cytochrome b (mt-cyt b) gene sequences were amplified by PCR, and then sequenced. Identity and phylogenetic analyses were performed using sequences obtained in this study and those from GenBank. Results Three hundred and seventy-four mammals, representing 2 order, 4 family, 6 genera, 9 species, were collected among which Apodemus agrariu and Myodes rutilus were the predominant species. The species identification based on morphological characters was in accordance with that based on molecular method. Conclusion In the Bolshoy Ussuriysky Island, small mammalian species are complex and diverse and their mt-cyt b genes had no obvious mutation. Many species can carry pathogens of natural focal diseases, so our study shed light on the prevention and control of these diseases.

Key words: Bolshoy Ussuriysky Island, Small mammals, Phylogenetic analysis

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