中国媒介生物学及控制杂志 ›› 2010, Vol. 21 ›› Issue (4): 312-313.

• 论著 • 上一篇    下一篇

德国小蠊乙酰胆碱酯酶活性的体躯分布和亚细胞分布

钱坤1,2, 高希武2, 曾晓芃1   

  1. 1. 北京市疾病预防控制中心消毒与有害生物防制所(北京 100013);
    2. 中国农业大学农学与生物技术学院(北京 100094)
  • 收稿日期:2010-03-02 出版日期:2010-08-20 发布日期:2010-08-20
  • 作者简介:钱坤(1978-),女,博士,助理研究员,主要从事媒介生物学研 究。Email: qkcandy@126.com

Body and subcellular distribution of AChE activities in Blattella germanica

QIAN Kun1,2, GAO Xi-wu2, ZENG Xiao-peng1   

  1. 1. Beijing Center for Disease Control and Prevention, Beijing 100013, China;
    2. College of Agriculture and Biotechnology, China Agricultural University, Beijing 100094, China
  • Received:2010-03-02 Online:2010-08-20 Published:2010-08-20

摘要:

目的 了解德国小蠊乙酰胆碱酯酶(AChE)活性的体躯分布和亚细胞分布。方法 利用不同体躯和不同亚细胞的AChE活性进行测定。结果 德国小蠊雄成虫的AChE在头部、胸部及腹部的活性分别占总活性的56.9%、19.5% 和23.6%。德国小蠊的AChE在头、胸、腹间的活性分布差异有统计学意义(F=127.96,P<0.05),头部AChE的活性显著高 于胸部和腹部(P<0.001),德国小蠊亚细胞AChE的总活性和比活力按大小排列依次为:线粒体>微粒体>胞质溶液>细胞核及细胞碎片。结论 德国小蠊的AChE在头部、胸部、腹部均有分布,但头部活性最高,占整体活性的50%以上。亚细 胞分布显示德国小蠊AChE在线粒体里分布最多,约占总活性的50%。

关键词: 德国小蠊, 乙酰胆碱酯酶, 体躯分布, 亚细胞分布

Abstract:

Objective To identify the body and subcellular distribution of acetylcholinesterase (AChE) activities in the Blattella germanica. Methods The AChE activities in different body parts and subcells were determined. Results The AChE activities in the head, chest and abdomin of male adults were 56.9%, 19.5% and 23.6% of the total activity, respectively. The difference in activity between the three body parts was statistically significant (F=127.96,P<0.05), where the AChE activity in the head was significantly higher than those in the chest and abdomen (P<0.001). The subcellular organelles in descending order of the total and specific AChE activity were: mitochondria>microsomes>cytoplasmic solution>nuclei and cell debris. Conclusion AChE activities were distributed in the head, chest and abdomen of B. germanica, with the highest in the head accounting for more than 50% of the overall activity. Subcellular distribution showed the highest AChE activity distribution in the mitochondria, accounting for almost 50% of the total activity.

Key words: Blattella germanica, Acetylcholinesterase, Body distribution, Subcellular distribution

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