关键词: 石松, 生物碱, 东乡伊蚊, 乙酰胆碱酯酶

Abstract: Objective To study the effect of Lycopodium clavatum L. Alkaloid on acetylcholinesterase(AChE) activity of Aedes togoi larvae. Methods Microplate test was used to detect activity of AChE in A.togoi larvae disposed at different dose alkaloid. Results In the groups disposed at low doses alkaloid of 0.625,1.25,2.50,5,10 mg/L,the activities of larvae AChE was in creased during 4 h,the absorbency values were 0.3714,0.3839,0.3752,0.3750,0.3882 respectively. but with the prolong of disposing time to 6 h,except the group at 0.625 mg/L,they decreased to a low point,the absorbency values were 0.3567,0.3176,0.2890,0.3020,respectively. And then,the activity of AChE raised gradually. High dose alkaloid (20 mg/L) depressed the activity of AChE in 6 h of disposing time and the absorbency values got to the lowest 0.3158 at 6 h,but with disposing time prolong,the activity of AChE raised gradually too. Conclusion L.clavatum L. alkaloid can restrain the activity of AChE in A.togoi larvae. The dose and disposing time should be taken in if the alkaloid is applied to control moisqutoes.