白纹伊蚊性别差异miRNA筛选及miRNA-mRNA调控网络分析

doi:10.11853/j.issn.1003.8280.2022.02.005

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (2): 191-200.DOI: 10.11853/j.issn.1003.8280.2022.02.005

白纹伊蚊性别差异miRNA筛选及miRNA-mRNA调控网络分析

刘文娟¹, 张可心¹, 程鹏², 张心雨¹, 张倩¹, 张忠¹, 张瑞玲¹

1. 山东第一医科大学基础医学院, 山东泰安 271016;
2. 山东第一医科大学寄生虫病防治研究所, 山东济宁 272033

收稿日期:2021-10-02 出版日期:2022-04-20 发布日期:2022-05-09
通讯作者: 张瑞玲,E-mail:zhangrl_06@126.com
作者简介:刘文娟,女,在读硕士,主要从事病媒生物与虫媒病研究,E-mail:lwj00002021@163.com
基金资助:
国家自然科学基金（81871686）

Deciphering sex differentially expressed miRNAs and miRNA-mRNA regulatory networks of Aedes albopictus

LIU Wen-juan¹, ZHANG Ke-xin¹, CHENG Peng², ZHANG Xin-yu¹, ZHANG Qian¹, ZHANG Zhong¹, ZHANG Rui-ling¹

1. School of Basic Medical Science, Shandong First Medical University, Tai'an, Shandong 271016, China;
2. Shandong Institute of Parasitic Diseases, Shandong First Medical University, Jining, Shandong 272033, China

Received:2021-10-02 Online:2022-04-20 Published:2022-05-09
Supported by:
Foundation:National Natural Sciences Foundation of China (No. 81871686)

摘要/Abstract

摘要： 目的研究雌、雄白纹伊蚊中差异表达的miRNA及其相对应的mRNA靶基因，进而探讨雌、雄白纹伊蚊miRNA-mRNA间的调控关系、预测关键基因的潜在功能，为阐明白纹伊蚊性别分化的调控机制积累资料。方法通过构建雌、雄白纹伊蚊miRNA文库和mRNA测序分析，分别得到雌、雄白纹伊蚊差异表达miRNA和mRNA信息，采用生物信息学方法预测差异表达miRNA的靶基因，将预测得到的miRNA的靶基因与雌、雄样品间差异表达mRNA进行重叠筛选，然后构建miRNA-mRNA互作关系网络图。进一步对miRNA对应的靶基因进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）功能富集分析，了解miRNA可能的功能。结果筛选到74个雌、雄差异表达miRNA，其中包含8个性别特异性miRNA。分别获得雌性特异表达的miRNA-mRNA 470对（miRNA低表达、mRNA高表达）和雄性特异表达的miRNA-mRNA 481对（miRNA高表达、mRNA低表达）。通过互作网络和功能富集分析，检测到4个与雌、雄白纹伊蚊性别分化及生殖功能相关的mRNA，以及直接参与调控它们的17个miRNA。结论构建了雌、雄白纹伊蚊样品中差异表达的miRNA-mRNA互作网络，筛选获得性别相关的17个miRNA和4个mRNA，为进一步验证miRNA在白纹伊蚊性别分化中的调控作用奠定基础。

关键词: 白纹伊蚊, 性别分化, miRNA-mRNA, 调控网络分析

Abstract: Objective To investigate differentially expressed miRNAs between male and female Aedes albopictus and their target mRNAs,explore miRNA-mRNA regulatory relationship in male and female Ae. albopictus,and predict the potential function of key genes, and to provide evidence for clarifying the regulatory mechanisms of sex differentiation of Ae. albopictus. Methods Through small RNA library construction and mRNA sequencing analysis,differentially expressed miRNAs and mRNAs between female and male Ae. albopictus were identified. Bioinformatic tools were used to predict the target mRNAs of the differentially expressed miRNAs. The miRNA-mRNA interaction networks were constructed using the overlapping results between the predicted target mRNAs and the sex differentially expressed mRNAs. GO and KEGG enrichment analysis were performed on the target mRNAs of miRNAs to predict the possible functions of miRNAs. Results A total of 74 sex differentially expressed miRNAs were screened out,including 8 sex-specific miRNAs. A total of 470 female-specific miRNA-mRNA pairs (down-regulated miRNAs,up-regulated mRNAs) and 481 male-specific miRNA-mRNA pairs (up-regulated miRNAs,down-regulated mRNAs) were acquired. The interaction network and functional enrichment analyses determined 4 mRNAs associated with the sex differentiation and reproduction of Ae. albopictus and 17 miRNAs that directly regulated these mRNAs. Conclusion We constructed sex differentially expressed miRNA-mRNA interaction networks of Ae. albopictus,and acquired 17 miRNAs and 4 mRNAs that were related to sex differentiation, laying the foundation for further research on the role of miRNAs in regulating the sex differentiation of Ae. albopictus.

Key words: Aedes albopictus, Sex differentiation, MiRNA-mRNA, Regulatory network analysis

中图分类号:

刘文娟, 张可心, 程鹏, 张心雨, 张倩, 张忠, 张瑞玲. 白纹伊蚊性别差异miRNA筛选及miRNA-mRNA调控网络分析[J]. 中国媒介生物学及控制杂志, 2022, 33(2): 191-200.

LIU Wen-juan, ZHANG Ke-xin, CHENG Peng, ZHANG Xin-yu, ZHANG Qian, ZHANG Zhong, ZHANG Rui-ling. Deciphering sex differentially expressed miRNAs and miRNA-mRNA regulatory networks of Aedes albopictus[J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 191-200.

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白纹伊蚊性别差异miRNA筛选及miRNA-mRNA调控网络分析

Deciphering sex differentially expressed miRNAs and miRNA-mRNA regulatory networks of Aedes albopictus

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