海口市及周边地区白纹伊蚊幼蚊肠道微生物分离与鉴定

doi:10.11853/j.issn.1003.8280.2022.02.006

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (2): 201-206.DOI: 10.11853/j.issn.1003.8280.2022.02.006

海口市及周边地区白纹伊蚊幼蚊肠道微生物分离与鉴定

覃晓宁¹, 任欣悦¹, 谢雪颜¹, 陈彦桦¹, 夏乾峰^1,2,3, 康迅^1,2,3

1. 海南医学院热带医学与检验医学院, 海南海口 571199;
2. 海南医学院热带转化医学教育部重点实验室, 海南海口 571199;
3. 海南医学院热带生物医学技术实验室, 海南海口 571199

收稿日期:2021-11-22 出版日期:2022-04-20 发布日期:2022-05-09
通讯作者: 康迅,E-mail:Kangxun2017@163.com
作者简介:覃晓宁,女,本科在读,主要从事病原微生物学研究工作,E-mail:1262162220@qq.com
基金资助:
校级大学生创新创业训练计划项目（X201911810115）

Isolation and identification of gut microbiota of Aedes albopictus larvae from Haikou and surrounding areas, China

TAN Xiao-ning¹, REN Xin-yue¹, XIE Xue-yan¹, CHEN Yan-hua¹, XIA Qian-feng^1,2,3, KANG Xun^1,2,3

1. School of Tropical Medicine and Laboratory Medicine, Hainan Medical College, Haikou, Hainan 571199, China;
2. Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical College, Haikou, Hainan 571199, China;
3. Tropical Biomedical Technology Laboratory, Hainan Medical College, Haikou, Hainan 571199, China

Received:2021-11-22 Online:2022-04-20 Published:2022-05-09
Supported by:
School-level College Students' Innovation and Entrepreneurship Training Program (No. X201911810115)

摘要/Abstract

摘要： 目的通过对海口市及周边5个地点的白纹伊蚊幼蚊肠道微生物的分离与鉴定，研究白纹伊蚊肠道微生物菌群的种类和多样性。方法 2019年在海口市及周边5个地点采集白纹伊蚊幼蚊；对幼蚊肠道微生物进行PCR扩增，产物测序，通过美国国立生物技术信息中心（NCBI）数据库对幼蚊和肠道微生物序列进行核苷酸序列同源性比对分析。结果白纹伊蚊幼蚊肠道微生物共鉴定出8株细菌，2株真菌，隶属于2门4科4属，优势菌群为厚壁菌门。海口市溪宜下村、大顶村，文昌市抱罗镇高堆村这3个地点的幼蚊肠道均分离出蜡状芽孢杆菌，仅临高县昌拱村的幼蚊中分离出真菌。海口市溪宜下村白纹伊蚊幼蚊肠道分离出的细菌种类最多，包括贝莱斯芽孢杆菌、纺锤形赖氨酸芽孢杆菌、蜡状芽孢杆菌和鲍曼不动杆菌。结论海口市及周边5个地点白纹伊蚊幼蚊肠道细菌以厚壁菌门细菌为主；肠道微生物细菌丰富度高于真菌。

关键词: 白纹伊蚊, 肠道微生物, 分离与鉴定, 菌落形态, 进化树

Abstract: Objective To study the species and diversity of gut microbiota of Aedes albopictus larvae collected in Haikou and five surrounding sites, China. Methods Ae. albopictus larvae were collected in Haikou and five surrounding sites in 2019. Polymerase chain reaction amplification and product sequencing were performed for gut microbiota of the larvae. The nucleotide sequences of the larvae and gut microbiota were analyzed for homology through the National Center for Biotechnology Information database. Results A total of 8 strains of bacteria and 2 strains of fungi, belonging to 4 genera in 4 families of 2 phyla, were identified in the gut microbiota of Ae. albopictus larvae, with the dominant microflora from the Firmicutes. Bacillus cereus was isolated from the intestinal tract of the larvae in Xiyixia village and Dading village of Haikou city, and Gaodui village, Baoluo town of Wenchang city Fungi were isolated only from the larvae in Changgong village of Lingao county. Ae. albopictus larvae in Xiyixia village, Haikou had the most species of bacteria isolated from the intestinal tract, including B. velezensis, Lysinibacillus fusiformis, B. cereus, and Acinetobacter baumannii. Conclusion Gut bacteria of Ae. albopictus larvae are mainly from the Firmicutes in Haikou and five surrounding sites. The species diversity of bacteria is greater than that of fungi among gut microbiota.

Key words: Aedes albopictus, Gut microbiota, Isolation and identification, Colony morphology, Phylogenetic tree

中图分类号:

R184.1
Q939

覃晓宁, 任欣悦, 谢雪颜, 陈彦桦, 夏乾峰, 康迅. 海口市及周边地区白纹伊蚊幼蚊肠道微生物分离与鉴定[J]. 中国媒介生物学及控制杂志, 2022, 33(2): 201-206.

TAN Xiao-ning, REN Xin-yue, XIE Xue-yan, CHEN Yan-hua, XIA Qian-feng, KANG Xun. Isolation and identification of gut microbiota of Aedes albopictus larvae from Haikou and surrounding areas, China[J]. Chinese Journal of Vector Biology and Control, 2022, 33(2): 201-206.

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海口市及周边地区白纹伊蚊幼蚊肠道微生物分离与鉴定

Isolation and identification of gut microbiota of Aedes albopictus larvae from Haikou and surrounding areas, China

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