尘螨分离及其变应原检测技术研究进展

doi:10.11853/j.issn.1003.8280.2021.06.024

摘要/Abstract

摘要： 尘螨种类多，分布广，是引起人类变态反应性疾病最常见变应原的来源生物之一。每克灰尘中含有2 μg的屋尘螨变应原第一组分就可能引起过敏及哮喘，达到10 μg（每克灰尘中含有500只螨虫）就被视为过敏体质者发生急性哮喘的主要风险因素。收集室内灰尘进行尘螨分离及其变应原检测，可用于评价尘螨孳生数量、分布情况，了解尘螨与变态反应性疾病的关系，有助于尘螨防控及变态反应性疾病研究。采用合适的方法对尘样进行收集和检测十分重要。该文对灰尘收集、尘螨分离和检测方法进行综述，为尘螨相关研究奠定基础。

关键词: 尘螨, 变应原, 免疫学, 分子生物学

Abstract: The diverse and widespread dust mites represent one of the most common allergens causing human allergic diseases. Two micrograms of Der p 1 per gram of dust may cause allergy and asthma, and 10 μg (500 mites per gram of dust) can be regarded as a main risk factor for acute asthma in patients with allergic constitution. Collecting indoor dust for dust mite isolation and allergen detection can be used to evaluate the number and distribution of dust mites, understand the relationship between dust mites and allergic diseases, and help control of dust mites and research on allergic diseases. Therefore, it is critical to adopt appropriate methods to collect and detect dust samples. Dust collection as well as dust mite isolation and detection methods are reviewed in this article to provide the foundation for dust mite research.

Key words: Dust mites, Allergen, Immunology, Molecular biology

中图分类号:

R384.4

沈鹏, 崔玉宝. 尘螨分离及其变应原检测技术研究进展[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 783-788.

SHEN Peng, CUI Yu-bao. Progress of research on dust mite isolation and allergen detection methods[J]. Chinese Journal of Vector Biology and Control, 2021, 32(6): 783-788.

参考文献

[1] Lei DK,Grammer LC. An overview of allergens[J]. Allergy Asthma Proc,2019,40(6):362-365.DOI:10.2500/aap.2019. 40.4247
[2] 张铃娟,李胜臻,崔玉宝. 浅谈防螨纺织品的发展现状[J]. 中国纤检,2019(10):124-126. DOI:10.3969/j.issn.1671-4466. 2019.10.039. Zhang LJ,Li SZ,Cui YB. Discussing on the development situation of anti-mite textiles[J]. Chin Fiber Insp,2019(10):124-126. DOI:10.3969/j.issn.1671-4466.2019.10.039.
[3] 李宛珈,黄志锋,廖陈喜,等. 广州地区2005例成人过敏性疾病患者过敏原特异性IgE检测结果分析[J]. 中国免疫学杂志,2020,36(22):2764-2768,2773. DOI:10.3969/j.issn.1000-484X.2020.22.015. Li WJ,Huang ZF,Liao CX,et al. Analysis of allergen-specific IgE test results in 2005 adults with allergic diseases in Guangzhou[J]. Chin J Immunol,2020,36(22):2764-2768,2773. DOI:10.3969/j.issn.1000-484X.2020.22.015.
[4] 桂碧玉,杨鹏,李立敬,等. 1920例过敏性疾病患者吸入性过敏原趋势分析[J]. 河南医学研究,2021,30(2):196-199. DOI:10.3969/j.issn.1004-437X.2021.02.002. Gui BY,Yang P,Li LJ,et al. Trend of inhaled allergen in 1920 patients with allergic diseases[J]. Henan Med Res,2021,30(2):196-199. DOI:10.3969/j.issn.1004-437X.2021.02.002.
[5] Noss I,Wouters IM,Visser M,et al. Evaluation of a low-cost electrostatic dust fall collector for indoor air endotoxin exposure assessment[J]. Appl Environ Microbiol,2008,74(18):5621-5627. DOI:10.1128/AEM.00619-08.
[6] Sander I,Lotz A,Zahradnik E,et al. Allergen quantification by use of electrostatic dust collectors (EDCs):influence of deployment time,extraction buffer,and storage conditions on the results[J]. Ann Occup Hyg,2016,60(7):845-859. DOI:10.1093/annhyg/mew027.
[7] Reboux G,Valot B,Rocchi S,et al. Storage mite concentrations are underestimated compared to house dust mite concentrations[J]. Exp Appl Acarol,2019,77(4):511-525. DOI:10.1007/s10493-019-00376-2.
[8] Lioy PJ,Freeman NC,Millette JR. Dust:a metric for use in residential and building exposure assessment and source characterization[J]. Environ Health Perspect,2002,110(10):969-983. DOI:10.1289/ehp.02110969.
[9] Adgate JL,Banerjee S,Wang M,et al. Performance of dust allergen carpet samplers in controlled laboratory studies[J]. J Expo Sci Environ Epidemiol,2013,23(4):385-391. DOI:10.1038/jes.2012.112.
[10] Sandel M,Murphy JS,Dixon SL,et al. A side-by-side comparison of three allergen sampling methods in settled house dust[J]. J Expo Sci Environ Epidemiol,2014,24(6):650-656.DOI:10. 1038/jes.2014.30.
[11] Townsend BT. Electrokinetic apparatus:US,73434258A[P]. 1965-06-01.
[12] Gordon J,Detjen P,Kelso D,et al. A new patient-operated sampling device for measurement of aeroallergens[J]. Ann Allergy Asthma Immunol,2016,116(5):475-476. DOI:10.1016/j.anai.2016.03.006.
[13] Richardson M,Gottel N,Gilbert JA,et al. Concurrent measurement of microbiome and allergens in the air of bedrooms of allergy disease patients in the Chicago area[J]. Microbiome,2019,7(1):82. DOI:10.1186/s40168-019-0695-5.
[14] Tovey ER,Liu-Brennan D,Garden FL,et al. Time-based measurement of personal mite allergen bioaerosol exposure over 24 hour periods[J]. PLoS One,2016,11(5):e0153414. DOI:10.1371/journal.pone.0153414.
[15] 裴伟. 尘螨分离技术的研究进展[J]. 热带医学杂志,2010,10(9):1149-1152. Pei W. Research progress on the isolation technology of house dust mite[J]. J Trop Med,2010,10(9):1149-1152.
[16] Sasa M,Matsumoto K,Miura A,et al. Saturated saline floatation method,a new and simple technic for the detection of grain mites in stored food products and drugs[J]. Jpn J Exp Med,1961,31:341-349.
[17] Sakaki I,Ito H,Suto C. An improved floatation method with saturated sodium chloride solution for isolating house dust mites[J]. Jpn J Sanit Zool,1991,42(1):43-46. DOI:10.7601/mez.42.43.
[18] Spieksma FT,Spieksma-Boezeman MI. The mite fauna of house dust with particular reference to the house-dust mite Dermatophagoides pteronyssinus (Trouessart,1897)(Psoroptidae:Sarcoptiformes)[J]. Acarologia,1967,9(1):226-241.
[19] 周鹰,顾晓虹,郁志伟,等. 一种收集灰尘中尘螨的方法及其应用:中国,201811311055.9[P]. 2019-04-05. Zhou Y,Gu XH,Yu ZW,et al. A method of dust mite collection from dust samples.:CN,201811311055.9[P]. 2019-04-05.
[20] Natuhara Y. New wet sieving method for isolating house dust mites[J]. Med Entomol Zool,1989,40(4):333-336. DOI:10.7601/mez.40.333.
[21] 崔玉宝,周鹰,田曼,等. 尘螨与变态反应性疾病[M]. 北京:科学出版社,2018:138. Cui YB,Zhou Y,Tian M,et al. Dust mites and allergic diseases[M]. Beijing:Science Press,2018:138.
[22] 胡慧中,湛孝东. 芜湖地区某高校宿舍螨类孳生情况调查[J]. 中国媒介生物学及控制杂志,2018,29(2):202-204. DOI:10.11853/j.issn.1003.8280.2018.02.024. Hu HZ,Zhan XD. Investigation on the prevalence of mites in a university dormitory in Wuhu city[J]. Chin J Vector Biol Control,2018,29(2):202-204. DOI:10.11853/j.issn.1003.8280. 2018.02.024.
[23] 罗述刚. 住宅建筑室内尘螨过敏原暴露与健康效应的研究[D]. 天津:天津大学,2018. Luo SG. Study on exposure of house dust mite allergens in residential buildings and its health effects[D]. Tianjin:Tianjin University,2018.
[24] 杨李,马桂芳,李启松,等. 超高倍多媒体显微诊断仪在腐食酪螨成螨形态鉴定中的应用[J]. 中国病原生物学杂志,2018,13(2):164-167. DOI:10.13350/j.cjpb.180213. Yang L,Ma GF,Li QS,et al. Use of a multimedia diagnostic microscope to morphologically identify adult Tyrophagus putrescentiae[J]. J Pathogen Biol,2018,13(2):164-167. DOI:10.13350/j.cjpb.180213.
[25] van Bronswijk JE,Bischoff E,Schirmacher W,et al. A rapid house-dust allergen test:preliminary results[J]. J Med Entomol,1986,23(2):217-218. DOI:10.1093/jmedent/23.2.217.
[26] van der Brempt X,Haddi E,Michel-Nguyen A,et al. Comparison of the ACAREX test with monoclonal antibodies for the quantification of mite allergens[J]. J Allergy Clin Immunol,1991,87(1):130-132. DOI:10.1016/0091-6749(91)90225-d.
[27] Tsay A,Williams L,Mitchell EB,et al. A rapid test for detection of mite allergens in homes[J]. Clin Exp Allergy,2002,32(11):1596-1601. DOI:10.1046/j.1365-2222.2002.01533.x.
[28] 王寅彪,刘肖,李青梅,等. 免疫层析试纸检测技术研究进展[J]. 河南科技大学学报(医学版),2017,35(3):236-240. DOI:10.15926/j.cnki.issn1672-688x.2017.03.022. Wang YB,Liu X,Li QM,et al. Research advance of immunochromatographic strip test[J]. J Henan Univ Sci Technol (Med Sci),2017,35(3):236-240. DOI:10.15926/j.cnki.issn1672-688x.2017.03.022.
[29] Platts-Mills TAE,Heymann PW,Chapman MD,et al. Cross-reacting and species-specific determinants on a major allergen from Dermatophagoides pteronyssinus and D. farinae:development of a radioimmunoassay for antigen P1 equivalent in house dust and dust mite extracts[J]. J Allergy Clin Immunol,1986,78(3):398-407. DOI:10.1016/0091-6749(86)90024-2.
[30] 张淑瑶,文霞,刘静霞,等. 空气净化器去除空气中尘螨过敏原的效果研究[J]. 工业微生物,2018,48(5):52-56. DOI:10.3969/j.issn.1001-6678.2018.05.011. Zhang SY,Wen X,Liu JX,et al. Removal effect of air cleaner on airborne mite allergens[J]. Ind Microbiol,2018,48(5):52-56. DOI:10.3969/j.issn.1001-6678.2018.05.011.
[31] 孙金霞,滕飞翔,崔玉宝. 尘螨变应原单克隆抗体的制备及应用研究进展[J]. 中国病原生物学杂志,2017,12(3):290-294. DOI:10.13350/j.cjpb.170322. Sun JX,Teng FX,Cui YB. Progress in preparation of monoclonal antibodies against dust mite allergens and their use[J]. J Pathogen Biol,2017,12(3):290-294. DOI:10.13350/j.cjpb. 170322.
[32] 曾颖,蔡玮红. 食品过敏原快速检测技术方法研究进展[J]. 包装与食品机械,2015,33(3):58-61. DOI:10.3969/j.issn.1005-1295.2015.03.014. Zeng Y,Cai WH. Research progress of rapid detection technologies and methods for alergen in food[J]. Packag Food Mach,2015,33(3):58-61. DOI:10.3969/j.issn.1005-1295. 2015.03.014.
[33] 王瑞琦,张宏誉. 放射过敏原吸附抑制实验评价过敏原提取液的总效价[J]. 中华临床免疫和变态反应杂志,2007,1(2):150-153. DOI:10.3969/j.issn.1673-8705.2007.02.005. Wang RQ,Zhang HY. Evaluate the total potency of allergen extract by radioallergosorbent test inhibition[J]. Chin J Allergy Clin Immunol,2007,1(2):150-153. DOI:10.3969/j.issn.1673-8705.2007.02.005.
[34] Swanson MC,Agarwal MK,Reed CE. An immunochemical approach to indoor aeroallergen quantitation with a new volumetric air sampler:studies with mite,roach,cat,mouse,and guinea pig antigens[J]. J Allergy Clin Immunol,1985,76(5):724-729. DOI:10.1016/0091-6749(85)90678-5.
[35] 俞黎黎,崔玉宝,王楠,等. 一种快速检测室尘中粉尘螨变应原第1组分含量的荧光实时定量PCR的方法:中国,201611137521. 7[P]. 2017-03-22. Yu LL,Cui YB,Wang N,et al. A RT-PCR method for rapid detection of Der f 1 in indoor dust:CN,201611137521. 7[P]. 2017-03-22.
[36] 许金鹏,李朝品. 屋尘螨和粉尘螨Ⅰ类变应原研究进展[J]. 中国媒介生物学及控制杂志,2009,20(5):494-496. Xu JP,Li CP. Research progress on the groupⅠallergen of house dust mite[J]. Chin J Vector Biol Control,2009,20(5):494-496.
[37] 刘娜娜,崔玉宝,叶林华,等. 高通量组学技术在尘螨研究中的应用进展[J]. 中国寄生虫学与寄生虫病杂志,2020,38(5):642-646. DOI:10.12140/j.issn.1000-7423.2020.05.018. Liu NN,Cui YB,Ye LH,et al. Applications of high-throughput omics technology in research on dust mite[J]. Chin J Parasitol Parasitic Dis,2020,38(5):642-646. DOI:10.12140/j.issn.1000-7423.2020.05.018.
[38] Platts-Mills TAE,de Weck AL,Aalberse RC,et al. Dust mite allergens and asthma:a worldwide problem[J]. J Allergy Clin Immunol,1989,83(2,Part 1):416-427.