空调隔尘网尘螨过敏原的检测

doi:10.11853/j.issn.1003.4692.2014.02.012

中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (2): 135-138.DOI: 10.11853/j.issn.1003.4692.2014.02.012

空调隔尘网尘螨过敏原的检测

王克霞¹, 刘志明², 姜玉新², 刁继东², 徐海丰¹, 徐鹏飞¹, 李朝品¹, ²

1 安徽理工大学医学院, 安徽淮南 232001;
2 皖南医学院医学寄生虫学教研室, 安徽芜湖 241002

收稿日期:2013-11-12 出版日期:2014-04-20 发布日期:2014-04-20
通讯作者: 李朝品, Email: cpli001@126.com
作者简介:王克霞, 女, 教授, 硕士生导师, 主要从事病原生物学和传染病学的教学与科研工作。Email: kxwang2009@126.com
基金资助:
国家自然科学基金(30872367, 81172790); 安徽省自然科学基金(070413088)

Detection of dust mite allergens from filters of air conditioners

WANG Kexia¹, LIU Zhiming², JIANG Yuxin², DIAO Jidong², XU Haifeng¹, XU Pengfei¹, LI Chaopin¹, ²

1 School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China;
2 Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, Anhui Province, China

Received:2013-11-12 Online:2014-04-20 Published:2014-04-20
Contact: LI Chaopin, Email: cpli001@126.com

摘要/Abstract

摘要： 目的检测安徽省芜湖地区家庭空调隔尘网灰尘和空调开机前、后室内空气中粉尘螨Ⅰ类过敏原(Der f 1)和屋尘螨Ⅰ类过敏原(Der p 1)浓度, 以探讨隔尘网中尘螨过敏原与哮喘发病的关系。方法分别从哮喘患者和健康居民家庭采集空调隔尘网灰尘样本各30份, 同时用粉尘采样器采集哮喘患者和健康居民室内空气粉尘样本, 开机前、后各采集30份。ELISA法检测Der f 1、Der p 1的浓度, Dot?ELISA检测灰尘提取液过敏原性。结果健康人群(家庭)组隔尘网灰尘中的过敏原Der f 1、Der p 1浓度中位数分别为 1.49和1.28 μg/g;哮喘患者(家庭)组隔尘网灰尘中的过敏原Der f 1、Der p 1浓度中位数分别为 0.73和0.85 μg/g, 二者差异有统计学意义(分别为P＜0.01, P＜0.05)。健康人群(家庭)组的空调开机前空气中Der f 1、Der p 1浓度中位数分别为 4.65和5.90 ng/m3, 开机后分别为 7.65和7.35 ng/m3。哮喘患者(家庭)组空调开机前空气中Der f 1、Der p 1浓度中位数分别为 5.05和5.90 ng/m3, 开机后分别为 7.15和7.10 ng/m3。检测结果显示, 打开空调送风情况下, 哮喘患者(家庭)组与健康人群(家庭)组的空气中Der f 1、Der p 1浓度均比未开空调时高, 且差异有统计学意义(P＜0.05)。Dot?ELISA分析显示, 灰尘提取液中过敏原能与螨过敏性哮喘患者的IgE产生结合反应。结论芜湖地区居民空调隔尘网中含有尘螨Ⅰ类过敏原, 空调开启送风后空气中尘螨的2 个主要过敏原浓度均显著升高, 应重视家用空调的清洁与净化, 定期清洗、更换隔尘网以防止或减少尘螨孳生。

关键词: 尘螨, 痒螨, 无气门股, 尘螨过敏, 过敏原, 空调隔尘网, 家螨

Abstract: Objective To determine the concentrations of allergens Der f 1 (from Dermatophagoides farinae) and Der p 1 (from D. pteronyssinus) in the dust from filters of domestic air conditioners and in the indoor air before and after the operation of air conditioners in Wuhu, Anhui province, China and to investigate the association of dust mite allergens in air conditioner filters with asthma attack. Methods Sixty dust samples were equally collected from the air conditioner filters in domestic houses of asthmatic patients and healthy subjects; indoor air dust samples were collected from the rooms of the above two groups using dust samplers, and for each group, 30 samples were collected before the operation of air conditioners and 30 samples after the operation. The concentrations of allergens Der f 1 and Der p 1 were determined by enzyme?linked immunosorbent assay (ELISA), and the binding capacity of allergens from dust extract was measured by dot?ELISA. Results The median concentrations of Der f 1 and Der p 1 in dust from filters were 1.49 and 1.28 μg/g for healthy subjects, versus 0.73 and 0.85 μg/g for asthmatic patients (P＜0.01 and P＜0.05). The median concentrations of Der f 1 and Der p 1 in air before the operation of conditioners were 4.65 and 5.90 ng/m3 for healthy subjects, versus 5.05 and 5.90 ng/m3 for asthmatic patients; the median concentrations of Der f 1 and Der p 1 in air after the operation of conditioners were 7.65 and 7.35 ng/m3 for healthy subjects, versus 7.15 and 7.10 ng/m3 for asthmatic patients. The Results showed that for both asthmatic patients and healthy subjects, the concentrations of Der f 1 and Der p 1 in air increased significantly after the air conditioners were turned on (P＜0.05). Additionally, the Dot?ELISA findings revealed that the allergens from dust extract were capable of binding to IgE from the sera of asthmatic patients allergic to dust mites. Conclusion Allergens Der f 1 and Der p 1 exist in the filters of air conditioners in domestic houses of Wuhu, and the concentrations of the two allergens in air increase significantly after the operation of air conditioners. These findings suggest that the filters of domestic air conditioners should be cleaned or replaced regularly to prevent or reduce internal accumulation of dust mites.

Key words: Dust mite, Psoroptidae, Astigmatina, Dust mite allergy, Allergen, Filter of air conditioner, Domestic mite

中图分类号:

R384.4

王克霞, 刘志明, 姜玉新, 刁继东, 徐海丰, 徐鹏飞, 李朝品,. 空调隔尘网尘螨过敏原的检测[J]. 中国媒介生物学及控制杂志, 2014, 25(2): 135-138.

WANG Kexia, LIU Zhiming, JIANG Yuxin, DIAO Jidong, XU Haifeng, XU Pengfei, LI Chaopin,. Detection of dust mite allergens from filters of air conditioners[J]. Chines Journal of Vector Biology and Control, 2014, 25(2): 135-138.

参考文献

[1] Asher MI, Montefort S, Bj?rkstén B, et al. Worldwide time trends in the prevalence of symptoms of asthma, allergic rhinoconjunctivitis, and eczema in childhood: ISAAC phases one and three repeat multicountry cross?sectional surveys[J]. Lancet, 2006, 368(9537):733-743.
[2]　Pawankar R, Baena?Cagnani CE, Bousquet J, et al. State of world allergy report 2008: allergy and chronic respiratory diseases[J]. World Allergy Organ J, 2008, 1(6 Suppl):S4-17.
[3]　Galli SJ, Tsai M, Piliponsky AM. The development of allergic inflammation[J]. Nature, 2008, 454(7203):445-454.
[4]　Terreehorst I, Oosting AJ, Tempels?Pavlica Z, et al. Prevalence and severity of allergic rhinitis in house dust mite?allergic patients with bronchial asthma or atopic dermatitis[J]. Clin Exp Allergy, 2002, 32(8):1160-1165.
[5]　Platts?Mills TA, Thomas WR, Aalberse RC, et al. Dust mite allergens and asthma: report of a second international workshop[J]. J Allergy Clin Immunol, 1992, 89(5):1046-1060.
[6]　许礼发, 王克霞, 赵军, 等. 空调隔尘网粉螨、真菌、细菌污染状况调查[J]. 环境与职业医学, 2008, 25(1):79-81.
[7]　Leech JA, Nelson WC, Burnett RT, et al. It’s about time: a comparison of Canadian and American time?activity patterns[J]. J Expo Anal Environ Epidemiol, 2002, 12(6):427-432.
[8]　Krantzs GW, Walter DE. A manual of acarology[M]. Lubbock: Texas Tech University Press, 2009:16-22.
[9]　沈莲, 孙劲旅, 陈军. 家庭致敏螨类概述[J]. 昆虫知识, 2010, 47(6):1264-1269.
[10]　石连, 姜玉新, 王海宁, 等. 粉尘螨变应原Der f 1基因的原核表达及多克隆抗体的制备[J]. 皖南医学院学报, 2012, 31(1):3-6.
[11]　Hallas TE. The biology of mites[J]. Allergy, 1991, 46 Suppl 11: S6-9.
[12]　Caplin J, Capriles?Hulett A, Iraola V, et al. Allergic sensitization to domestic mites in Corpus Christi, Texas [J]. Allergy Asthma Proc, 2009, 30:166-170.
[13]　Thomas WR, Smith WA, Hales BJ. The allergenic specificities of the house dust miet[J]. Chang Gung Med J, 2004, 27(8):563.
[14]　杨庆贵, 李朝品. 粉尘螨Ⅰ类变应原的cDNA克隆测序及亚克隆[J]. 中国寄生虫学与寄生虫病杂志, 2004, 22(3):173-175.
[15]　Platts?Mills TA, Thomas WR, Aalberse RC, et al. Dust mite allergens and asthma: Report of a second international workshop[J]. J Allergy Clin Immunol, 1992, 89(5):1046-1060.
[16]　Zheng YW, Li J, Lai XX, et al. Allergen micro?array detection of specific IgE?reactivity in Chinese allergy patients[J]. Chin Med J, 2011, 124(24):4350-4354.
[17]　Hales BJ, Martin AC, Pearce LJ, et al. IgE and IgG anti?house dust mite specificities in allergic disease[J]. J Allergy Clin Immunol, 2006, 118(2):361-367.
[18]　李朝品. 医学蜱螨学[M]. 北京:人民军医出版社, 2006:229-299.
[19]　湛孝东, 陈琪, 郭伟, 等. 芜湖地区居室空调粉螨污染研究[J]. 中国媒介生物学及控制杂志, 2013, 24(4):301-303.
[20]　Sublett JL, Seltzer J, Burkhead R, et al. Air filters and air cleaners: Rostrum by the American Academy of allergy, asthma & immunology indoor allergen committee[J]. J Allergy Clin Immunol, 2010, 125(1):32-38.
[21]　Vichyanond P, Pensrichon R, Kurasirikul S. Progress in the management of childhood asthma[J]. Asia Pac Allergy, 2012, 2(1):15-25.
[22]　Sih T, Mion O. Allergic rhinitis in the child and associated comorbidities[J]. Pediatr Allergy Immunol, 2010, 21(1 Pt 2): e107-113.
[23]　Prescott SL. Maternal allergen exposure as a risk factor for childhood asthma[J]. Curr Allergy Asthma Rep, 2006, 6(1): 75-80.
[24]　Liu ZG, Bai Y, Ji KM, et al. Detection of Dermatophagodes farinae in the dust of air?conditionging filters[J]. Int Arch Allergy Immunol, 2007, 144(1):85-90.

空调隔尘网尘螨过敏原的检测

Detection of dust mite allergens from filters of air conditioners

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