Effects of pebble and pine bark mulches on Axonopus compressus grassland for Forcipomyia taiwana control

doi:10.11853/j.issn.1003.8280.2022.04.026

Abstract

Abstract: Objective To study a technique for the control of Forcipomyia taiwana on Axonopus compressus grassland in an urban park. Methods On April 15, 2021, four types of grass trays where F. taiwana could oviposit and breed were placed in the outdoor environment of Futian Mangrove Ecological Park, Shenzhen, China:an A. compressus tray with a soil mulch of small pebbles, an A. compressus tray with a mulch of broken pine barks, an A. compressus tray without any soil mulch, and a Zoysia tenuifolia tray. After 75 days, these trays were moved to the laboratory of School of Life Sciences, Sun Yat-sen University, and were placed into insect cages to rear F. taiwana. Adult F. taiwana midges were collected and counted by sex. The emergence of adult F. taiwana was compared using non-parametric tests and analysis of variance between A. compressus trays with pebble and pine bark mulches, and using the two-sample t test between A. compressus and Z. tenuifolia trays without any soil mulch. One-way analysis of variance was used to compare the numbers of male and female adult F. taiwana emerging from the trays with different soil mulches. Results There was a statistical difference in the numbers of adult F. taiwana emerging from the pine bark-mulched A. compressus tray (3.00±0.92), pebble-mulched A. compressus tray (8.50±2.37), and A. compressus tray without any mulch (26.00±7.23) (F=6.735, P=0.007). The number of adult F. taiwana was higher in the A. compressus tray without any mulch (26.00±7.23) than in the Z. tenuifolia tray (12.00±3.33), but without statistical significance (t=1.719, P=0.111). There was no statistical difference in the number of male and female adult F. taiwana from any grass tray (all P>0.05). Conclusion Applying a pebble or pine bark mulch onto A. compressus grassland is effective to control F. taiwana, and the pine park is superior to pebbles. Compared with A. compressus grassland, Z. tenuifolia grassland cannot significantly reduce the breeding density of F. taiwana.

Key words: Forcipomyia taiwana, Control, Pebble, Pine bark

摘要： 目的研究城市公园内人工种植的大叶油草地控制台湾铗蠓的技术方法。方法 2021年4月15日，在深圳市福田红树林生态公园户外放置4种台湾铗蠓可在其中产卵孳生的草盘（大叶油草土壤表面铺设小石卵、大叶油草土壤表面铺设松木皮、大叶油草、细叶结缕草）。75 d后移入中山大学（南校区）生命科学学院实验室，在养虫笼中培养草盘中羽化出的台湾铗蠓，统计台湾铗蠓成虫数量并区分雌雄。采用非参数检验和方差分析比较铺设小石卵和松木皮的大叶油草盘中台湾铗蠓的羽化量差异；采用双样本t检验比较台湾铗蠓在大叶油草和细叶结缕草草盘上的羽化量差异；采用单因素方差分析不同土壤覆盖物条件下的台湾铗蠓雌雄羽化数量差异。结果铺设松木皮和铺设小石卵的大叶油草盘与仅有大叶油草的草盘之间台湾铗蠓的羽化数量分别为（3.00±0.92）、（8.50±2.37）和（26.00±7.23）只，差异有统计学意义（F=6.735，P=0.007）。不铺设任何土壤覆盖物的大叶油草草盘中台湾铗蠓的羽化数量[（26.00±7.23）只]高于细叶结缕草草盘[（12.00±3.33）只]，但差异无统计学意义（t=1.719，P=0.111）。不同土壤覆盖物条件下台湾铗蠓雌雄羽化数量差异无统计学意义（均P>0.05）。结论在大叶油草地上铺放小石卵和松木皮对防制台湾铗蠓有效，其中松木皮效果好于小石卵。与大叶油草地相比，细叶结缕草地不能明显降低台湾铗蠓的孳生密度。

关键词: 台湾铗蠓, 防制, 小石卵, 松木皮

CLC Number:

R384.5

CHEN Jia-hui, ZHOU Jing-ya, LIU Yang, TANG Jin-hua, XIE Kai-qi, ZHANG Shao-hua, JIA Feng-long. Effects of pebble and pine bark mulches on Axonopus compressus grassland for Forcipomyia taiwana control[J]. Chinese Journal of Vector Biology and Control, 2022, 33(4): 586-589.

陈家慧, 周静雅, 刘阳, 唐瑾华, 谢恺琪, 张韶华, 贾凤龙. 大叶油草地铺小石卵和松木皮防制台湾铗蠓的效果研究[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 586-589.

References

[1] 虞以新. 中国蠓科昆虫[M]. 北京:军事医学科学出版社, 2006:1647-1649. Yu YX. Ceratopogonidae of China[M]. Beijing:Military Medical Science Press, 2006:1647-1649. (in Chinese)
[2] Strickman D, Wirtz R, Lawyer P, et al. Meteorological effects on the biting activity of Leptoconops americanus (Diptera:Ceratopogonidae)[J]. J Am Mosq Control Assoc, 1995, 11(1):15-20.
[3] Tabachnick WJ. Culicoides and the global epidemiology of bluetongue virus infection[J]. Vet Ital, 2004, 40(3):144-150.
[4] Velthuis AGJ, Saatkamp HW, Mourits MCM, et al. Financial consequences of the Dutch bluetongue serotype 8 epidemics of 2006 and 2007[J]. Prev Vet Med, 2010, 93(4):294-304. DOI:10.1016/j.prevetmed.2009.11.007.
[5] 范娜, 程睿, 鲁晓晴, 等. 蠓虫及其传播的虫媒病毒[J]. 中国热带医学, 2018, 18(2):182-187. DOI:10.13604/j.cnki.46-1064/r.2018.02.22. Fan N, Cheng R, Lu XQ, et al. Ceratopogonidae and their transmitted arbovirus[J]. China Trop Med, 2018, 18(2):182-187. DOI:10.13604/j.cnki.46-1064/r.2018.02.22.(in Chinese)
[6] 何于雯, 李楠, 孟锦昕, 等. 云南省芒市采集蠓中辛德毕斯病毒的分离与鉴定[J]. 中国媒介生物学及控制杂志, 2021, 32(4):498-502. DOI:10.11853/j.issn.1003.8280.2021.04.023. He YW, Li N, Meng JX, et al. Isolation and identification of Sindbis virus from midges collected in Mangshi, Yunnan province, China[J]. Chin J Vector Biol Control, 2021, 32(4):498-502. DOI:10.11853/j.issn.1003.8280.2021.04.023.(in Chinese)
[7] 王琴燕, 付士红, 孙定炜, 等. 海南省野生蠓虫西藏环状病毒的研究[J]. 中国媒介生物学及控制杂志, 2021, 32(4):415-421. DOI:10.11853/j.issn.1003.8280.2021.04.006. Wang QY, Fu SH, Sun DW, et al. A study of wild midges carrying Tibet orbivirus in Hainan province, China[J]. Chin J Vector Biol Control, 2021, 32(4):415-421. DOI:10.11853/j.issn.1003.8280. 2021.04.006.(in Chinese)
[8] Barceló C, Purse BV, Estrada R, et al. Environmental drivers of adult seasonality and abundance of biting midges Culicoides (Diptera:Ceratopogonidae), bluetongue vector species in Spain[J]. J Med Entomol, 2021, 58(1):350-364. DOI:10.1093/jme/tjaa160.
[9] Sakkas H, Bozidis P, Franks A, et al. Oropouche fever:A review[J]. Viruses, 2018, 10(4):175. DOI:10.3390/v10040175.
[10] 刘金华, 郝丽, 刘世忠, 等. 广东省蠓科昆虫的种类与地理分布[J]. 中华卫生杀虫药械, 2014, 20(3):275-278. DOI:10. 19821/j.1671-2781.2014.03.027. Liu JH, Hao L, Liu SZ, et al. Species and geographic distribution of Ceratopogonidae in Guangdong province[J]. Chin J Hyg Insect Equip, 2014, 20(3):275-278. DOI:10.19821/j.1671-2781.2014. 03.027.(in Chinese)
[11] 连日清. 台湾铗蠓属蠛蠓亚属蠓种的分类及生态研究[J]. 台湾省立博物馆半年刊, 1989, 42(1):37-77. DOI:10.6532/JTM.198906_42(1).0006. Lien JC. Taxonomic and ecological studies on the biting midges of the subgenus Lasiohelea, genus Forcipomyia from Taiwan[J]. J Taiwan Museum, 1989, 42(1):37-77. DOI:10.6532/JTM. 198906_42(1).0006.(in Chinese)
[12] 柳忠婉, 丁尔成, 蔡连来, 等. 台湾蠛蠓孳生地调查[J]. 昆虫学报, 1964, 13(5):123-126.DOI:10.16380/j.kcxb.1964.05.010. Liu ZW, Ding EC, Cai LL, et al. Observation on the breeding habits of Lasiohelea taiwana Shiraki[J]. Acta Entomol Sin, 1964, 13(5):123-126.DOI:10.16380/j.kcxb.1964.05.010.(in Chinese)
[13] 裘明华. 台湾铗蠓多次取食习性的观察(双翅目:蠓科)[J]. 昆虫学报, 1981, 24(2):228-230. DOI:10.16380/j.kcxb.1981. 02.020. Jeu MH. Observations on multiple feeding habits of Forcipomyia (Lasiohelea) taiwana (Shiraki)[J]. Acta Entomol Sin, 1981, 24(2):228-230. DOI:10.16380/j.kcxb.1981.02.020.(in Chinese)
[14] 裘明华, 荣云龙. 台湾铗蠓的生活史研究(双翅目:蠓科)[J]. 昆虫学报, 1979, 22(4):437-442. DOI:10.16380/j.kcxb.1979. 04.009. Jeu MH, Rong YL. Studies on the life history of Forcipomyia (Lasiohelea) taiwana (Shiraki) (Diptera:Ceratopogonidae)[J]. Acta Entomol Sin, 1979, 22(4):437-442. DOI:10.16380/j.kcxb. 1979.04.009.(in Chinese)
[15] 裘明华, 荣云龙. 台湾铗蠓幼期的形态(双翅目:蠓科)[J]. 昆虫学报, 1980, 23(1):66-75. DOI:10.16380/j.kcxb.1980.01.010. Jeu MH, Rong YL. Morphological descriptions of the immature stages of Forcipomyia (Lasiohelea) taiwana (Shiraki) (Diptera:Ceratopogonidae)[J]. Acta Entomol Sin, 1980, 23(1):66-75. DOI:10.16380/j.kcxb.1980.01.010.(in Chinese)
[16] 王玮龙, 温育德, 林宗岐. 南投埔里桃米社区台湾铗蠓(Forcipomyia taiwana)(双翅目:蠓科)密度与藻类之关系[J]. 生物科学, 2010, 52(2):25-37. DOI:10.29981/CB.201012.0003. Wang WL, Wen YD, Lin ZQ. The relationship of biting midges (Forcipomyia taiwana) (Diptera:Ceratopogonidae) density with algae on habitat Tao-Mi village, Puli township in Nantou county[J]. Chin Biosci, 2010, 52(2):25-37. DOI:10.29981/CB. 201012.0003.(in Chinese)
[17] Luo YP. Establishing and maintaining colonies of Forcipomyia taiwana in the laboratory[J]. J Vector Ecol, 2018, 43(2):328-333. DOI:10.1111/jvec.12317.
[18] Shih CL, Liao QM, Wang YY, et al. Abundance and host-seeking activity of the biting midge, Forcipomyia taiwana (Diptera:Ceratopogonidae)[J]. J Asia-Pac Entomol, 2019, 22(4):1053-1059. DOI:10.1016/j.aspen.2019.08.014.
[19] 陈家慧, 谢恺琪, 唐佳梦, 等. 深圳市福田红树林生态公园吸血蠓种类及吸血活动研究[J]. 环境昆虫学报, 2021, 43(2):373-378. DOI:10.3969/j.issn.1674-0858.2021.02.11. Chen JH, Xie KQ, Tang JM, et al. Studies on species and activities of blood-sucking midges in Futian Mangrove Ecological Park, Shenzhen[J]. J Environ Entomol, 2021, 43(2):373-378. DOI:10.3969/j.issn.1674-0858.2021.02.11.(in Chinese)
[20] 陈家慧, 刘阳, 谢恺琪, 等. 土壤湿度对台湾铗蠓Forcipomyia taiwana (Shiraki)产卵与幼虫发育影响[J]. 中山大学学报(自然科学版), 2021, 60(4):12-18. DOI:10.13471/j.cnki.acta.snus.2021E012. Chen JH, Liu Y, Xie KQ, et al. Effects of soil humidity on the oviposition and larval development of the Forcipomyia taiwana (Shiraki)[J]. Acta Sci Nat Univ Sunyatseni, 2021, 60(4):12-18. DOI:10.13471/j.cnki.acta.snus.2021E012.(in Chinese)
[21] 宋福春, 贺骥. 吸血蠓的危害及防治[J]. 中华卫生杀虫药械, 2015, 21(6):623-626. DOI:10.19821/j.1671-2781.2015.06.027. Song FC, He J. Harm and control of blood-sucking midges[J]. Chin J Hyg Insect Equip, 2015, 21(6):623-626. DOI:10.19821/j.1671-2781.2015.06.027.(in Chinese)