Chines Journal of Vector Biology and Control ›› 2018, Vol. 29 ›› Issue (6): 582-585.DOI: 10.11853/j.issn.1003.8280.2018.06.007

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Morphological and DNA barcoding identification of the indoor Ameroseius plumosus

WANG Yi-xiao1, ZHENG Ling-xiao2, GUAN Guo-yu1, YIN Can-can2, LIU Xiao1, WANG Shu-ran1, WEN Zi-ang1, CHEN Ying-wei2, CHENG Xu3, SUN En-tao2   

  1. 1 School of Clinical Medicine, Wannan Medical College, Wuhu 241002, Anhui Province, China;
    2 School of Laboratory Medicine, Wannan Medical College;
    3 School of Traditional Chinese Medicine and Western Medicine, Anhui University of Chinese Medicine
  • Received:2018-06-20 Online:2018-12-20 Published:2018-12-20
  • Supported by:
    Supported by the Key Program in the Youth Elite Support Plan in Universities of Anhui Province (No. gxyqZD2016174),the Major Project of Education Department in Anhui (No. KJ2016A725) and the Project of Innovation and Entrepreneurship for Undergraduates in Anhui (No. 201710368067)


王逸枭1, 郑凌霄2, 管国宇1, 尹灿灿2, 刘啸1, 汪书然1, 温子昂1, 陈樱玮2, 程序3, 孙恩涛2   

  1. 1 皖南医学院临床医学院, 安徽 芜湖 241002;
    2 皖南医学院检验学院, 安徽 芜湖 241002;
    3 安徽中医药大学中西医结合学院, 安徽 合肥 230038
  • 通讯作者: 孙恩涛,
  • 作者简介:王逸枭,男,主要从事病原生物学研究,
  • 基金资助:

Abstract: Objective To identify the mites accurately and rapidly. Methods Most of the mites seized on a plush toy in a Children's room in Wuhu in July 2016 were identified by morphology. Six individuals were amplified by the cytochrome C oxidase subunit I (COⅠ) gene. Meanwhile, based on the gene, phylogenetic tree of the known Ameroseiidae mites was constructed by Neighbor-Joining method. Results According to morphological observations, the mite we seized was identified as female Ameroseius plumosus, belonging to Ameroseiidae, Ascoidea, Gamasina. The DNA barcode of the COⅠgene was obtained from the mite for the first time. The six individuals were identified as the same species because all the inter-individual's DNA barcode differences were less than 0.5%. Phylogenetic tree shows that A. plumosus is associated with other individuals of Ameroseiidae. Conclusion It's the first time that the A. plumosus was found on a plush toy. Combining morphology and DNA barcode techniques together could provide a good tool for the identification of the medical vectors rapidly and accurately.

Key words: Ameroseius plumosus, DNA barcode, Morphology, Identification, Phylogenetic tree

摘要: 目的 准确快速鉴定居室内检获的螨类。方法 对2016年7月于芜湖市某儿童房内毛绒玩具上检获的大部分螨类进行形态鉴定,对6只螨扩增线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因,基于该基因以邻接法构建已知美绥螨科螨类的系统进化树。结果 经形态鉴定,检获螨类为雌性羽美绥螨(Ameroseius plumosus),属革螨股、囊螨总科、美绥螨科。首次扩增了羽美绥螨COⅠ基因的DNA条形码,6只个体间的DNA条形码差异<0.5%,判断这6只个体为同一种类。系统进化树显示羽美绥螨与美绥螨科的种类聚在一支。结论 首次在毛绒玩具中发现羽美绥螨。应用形态鉴定和DNA条形码相结合的鉴定方法,可有效提高医学媒介生物种类鉴别能力和准确度。

关键词: 羽美绥螨, DNA条形码, 形态, 鉴定, 系统进化树

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