A specimen preparation method for human Demodex

doi:10.11853/j.issn.1003.8280.2022.01.026

Chinese Journal of Vector Biology and Control ›› 2022, Vol. 33 ›› Issue (1): 143-147.DOI: 10.11853/j.issn.1003.8280.2022.01.026

• Technology and Method • Previous Articles Next Articles

A specimen preparation method for human Demodex

WU Hai¹, ZHONG Sai-feng², ZHAO Qiu-yu¹, QUAN Yun-fan¹

1. School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 571199, China;
2. School of Basic Medicine and Life Science, Hainan Medical University, Haikou, Hainan 571199, China

Received:2021-06-04 Online:2022-02-20 Published:2022-02-17
Supported by:
Undergraduate Innovation and Entrepreneurship Training Program of Hainan (No. S202011810007); Research Project of Education and Teaching Reform in Colleges and Universities of Hainan (No. Hnjg2021-67); Educational Research Projects of Hainan Medical University (No. HYYB202008)

人体蠕形螨标本制作方法探讨

吴海¹, 钟赛凤², 赵秋宇¹, 权云帆¹

1. 海南医学院热带医学与检验医学院, 海南海口 571199;
2. 海南医学院基础医学与生命科学学院, 海南海口 571199

通讯作者: 权云帆,E-mail:quanyunfannpc@126.com
作者简介:吴海,男,本科在读,从事医学检验技术专业相关研究,E-mail:1760158434@qq.com。
基金资助:
海南省大学生创新创业训练计划项目（S202011810007）；海南省高等学校教育教学改革研究项目（Hnjg2021-67）；海南医学院教育科研项目（HYYB202008）

Abstract

Abstract: Objective To establish a safe and rapid preparation method for long-term preservation of human Demodex specimen, and to meet the needs of experimental teaching of human parasitology. Methods Human Demodex mites were obtained by the cellophane tape method and the squeezing method and were treated with various methods to compare the sealing effect of neutral balsam, odorless mounting medium, and glycerol jelly mounting medium, and the morphology of mite was observed and photographed under the KoPa WiFi EDU software system of the digital microscope. Results The specimens with few background impurities or little grease which did not affect observation were directly sealed with odorless mounting medium or glycerol jelly mounting medium. In contrast, the specimens with excessive bubbles, grease, or hair which affected observation were first cleaned with 2% dish soap for a short period of time, then placed in 75%-100% ethanol for dehydration, and finally sealed with odorless mounting medium. If there was a high density of mites on one sheet of adhesive tape, such specimens were first soaked in 2% dish soap for a long time and then centrifuged, and the mites collected were fixed with 75% ethanol and sealed with a glycerol jelly mounting medium. Conclusion Odorless mounting medium or glycerol jelly mounting medium can replace neutral balsam as the mounting medium for preparing Demodex specimen, and 2% dish soap can remove the excess grease in specimen and easily release the mites from the adhesive tape.

Key words: Demodex folliculorum, Demodex brevis, Morphology, Specimen preparation

摘要： 目的探索和研究安全、快速，且能长期保存人体蠕行螨标本的制作方法，以期满足人体寄生虫实验教学的需要。方法采用透明胶纸粘贴法和挤刮涂片法获取蠕形螨，使用多种方法对人体蠕形螨进行处理，对比中性树胶、无味型封片剂以及甘油明胶的封片效果，在数码显微镜KoPa WiFi EDU软件系统下观察和拍摄。结果背景杂质少油脂少不影响观察的标本可直接采用无味型封片剂或者甘油明胶对透明胶封片保存。透明胶未粘贴好导致气泡多或者油脂、汗毛多影响观察的标本可采用2%洗洁精短时间清洁后，依次放入浓度为75%～100%的乙醇溶液中脱水后，采用无味型封片剂封片保存。对于透明胶上密度较大的标本，可采用2%洗洁精长时间浸泡后，离心沉淀，取沉渣放入75%乙醇溶液中固定后，采用甘油明胶封片保存。结论可采用无味型封片剂或者甘油明胶代替中性树胶作为蠕形螨标本制作的封片剂。采用2%洗洁精可清洗标本中存在的油脂且可达到从透明胶带中游离蠕形螨的效果。

关键词: 毛囊蠕形螨, 皮脂蠕形螨, 形态, 标本制作

CLC Number:

R384.4

WU Hai, ZHONG Sai-feng, ZHAO Qiu-yu, QUAN Yun-fan. A specimen preparation method for human Demodex[J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 143-147.

吴海, 钟赛凤, 赵秋宇, 权云帆. 人体蠕形螨标本制作方法探讨[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 143-147.

References

[1] 卢致民,李凤铭. 临床寄生虫学检验技术[M]. 武汉:华中科技大学出版社,2019:213-214. Lu ZM,Li FM. Clinical parasitology and laboratory technology[M]. Wuhan:Huazhong University of Science & Technology Press,2019:213-214. (in Chinese)
[2] 蒲兴旺,刘刚,鲁开化. 人类蠕形螨虫与皮肤健康的临床研究进展[J]. 中国美容整形外科杂志,2018,29(8):510-511. DOI:10.3969/j.issn.1673-7040.2018.08.019. Pu XW,Liu G,Lu KH. Progress in clinical research on human Demodex and skin health[J]. Chin J Aesth Plast Surg,2018,29(8):510-511. DOI:10.3969/j.issn.1673-7040.2018.08.019.(in Chinese)
[3] 诸欣平,苏川. 人体寄生虫学[M]. 9版. 北京:人民卫生出版社,2018:245. Zhu XP,Su C. Human parasitology[M]. 9th ed. Beijing:People's Medical Publishing House,2018:245. (in Chinese)
[4] Hellerich U,Metzelder M. Incidence of scalp involvement by Demodex folliculorum Simon ectoparasites in a pathologic-anatomic and forensic medicine autopsy sample[J]. Arch Kriminol,1994,194(3/4):111-118.
[5] Madeira NG,Sogayar MIL. Prevalencia de Demodex folliculorum e Demodex brevis em Uma amostra da população de Botucatu,São Paulo,Brasil[J]. Rev Soc Bras Med Trop,1993,26(4):221-224. DOI:10.1590/S0037-86821993000400004.
[6] 李朝品. 医学蜱螨学[M]. 北京:人民军医出版社,2006:299-322. Li CP. Medical acarology[M]. Beijing:People's Military Medical Publishing House,2006:299-322. (in Chinese)
[7] 郭家,郭俊杰,张浩,等. 制备蠕形螨玻片标本的新方法:透明胶纸永久封片法[J]. 齐齐哈尔医学院学报,1999,20(5):507-508. Guo J,Guo JJ,Zhang H,et al. A new method for preparing Demodex glass slides:permanent mounting cellophane tape[J]. J Qiqihar Med Univ,1999,20(5):507-508. (in Chinese)
[8] 王唯唯,黄文达. 中性树胶直接封片制作蠕形螨标本[J]. 中国寄生虫学与寄生虫病杂志,1998,16(2):76. Wang WW,Huang WD. Preparation of Demodex specimen by direct mounting with neutral gum[J]. Chin J Parasitol Parasit Dis,1998,16(2):76. (in Chinese)
[9] 李永祥,张辉,刘振忠,等. 蠕形螨标本制作方法探讨[J]. 中国寄生虫学与寄生虫病杂志,2002,20(4):196. DOI:10.3969/j.issn.1000-7423.2002.04.018. Li YX,Zhang H,Liu ZZ,et al. Preparation and staining of Demodex folliculorum specimen[J]. Chin J Parasitol Parasit Dis,2002,20(4):196. DOI:10.3969/j.issn.1000-7423.2002.04.018.(in Chinese)
[10] 李永祥,刘振忠,郑志红. 蠕形螨标本的制作与染色[J]. 中国人兽共患病杂志,2002,18(2):12. DOI:10.3969/j.issn.1002-2694.2002.02.048. Li YX,Liu ZZ,Zheng ZH. Demodicidae sample making[J]. Chin J Zoonoses,2002,18(2):12. DOI:10.3969/j.issn.1002-2694. 2002.02.048.(in Chinese)
[11] 刘莹,李速婷. 人体蠕形螨环境扫描电镜样本的制作[J]. 医学动物防制,2007,23(11):818-819. DOI:10.3969/j.issn.1003-6245.2007.11.008. Liu Y,Li ST. Preparation of environmental scanning electron microscope samples of human Demodex[J]. Med Ani Prev,2007,23(11):818-819. DOI:10.3969/j.issn.1003-6245.2007.11.008.(in Chinese)

[1]	WANG Chao, YAN Li-ping, LI Xin-yu, ZHANG Dong, LIU Qi-yong. Research advances of Sarcophaga in China [J]. Chinese Journal of Vector Biology and Control, 2022, 33(1): 153-158.
[2]	LI Cun-xiang, ZHANG Qing-wen, XIN You-quan, QI Zhi-zhen, JIN Xing, FENG Jian-ping, LI Sheng, WU Hai-lian, ZHAO Hai-hong, DAI Rui-xia. A study of colonial morphology of Yersinia pestis carrying bacteriophages [J]. Chines Journal of Vector Biology and Control, 2020, 31(6): 699-701.
[3]	LIU De-xing, QIU De-yi, LI Ting-ting, ZHANG Rui-ling, LIU Chun-xiao, YUE Qiao-yun. Morphological and molecular identification of Cimex hemipterus and C. lectularius [J]. Chines Journal of Vector Biology and Control, 2018, 29(6): 576-581.
[4]	WANG Yi-xiao, ZHENG Ling-xiao, GUAN Guo-yu, YIN Can-can, LIU Xiao, WANG Shu-ran, WEN Zi-ang, CHEN Ying-wei, CHENG Xu, SUN En-tao. Morphological and DNA barcoding identification of the indoor Ameroseius plumosus [J]. Chines Journal of Vector Biology and Control, 2018, 29(6): 582-585.
[5]	WEI Xiao-ya, LIU De-xing, QIU De-yi, YUE Qiao-yun. Identification of Ceratinoptera picta: a non-recorded cockroach species in China intercepted at Zhongshan port, China [J]. Chines Journal of Vector Biology and Control, 2018, 29(1): 23-26.
[6]	LI Ji-xu, SONG Zhen-hai, PIAO Guang-ming. Species identification of Anopheles mosquitoes from the Tumen river basin in Yanbian area of Jilin province based on morphological and molecular characteristics [J]. Chines Journal of Vector Biology and Control, 2018, 29(1): 42-46.
[7]	QIU De-yi, WEI Xiao-ya, WU Ke-liang, YUE Qiao-yun. Morphological and molecular identification of Blattella germanica and its closely related species [J]. Chines Journal of Vector Biology and Control, 2017, 28(3): 220-225.
[8]	WEI Xiao-ya, QIU De-yi, YUE Qiao-yun, LIU De-xing. DNA barcoding and morphology identification of Pollenia rudis－a non-recorded fly species in China [J]. Chines Journal of Vector Biology and Control, 2016, 27(4): 326-329.
[9]	YAO Xuan, XIONG Jin-feng, GUAN Xu-hua. Survey on mosquito species in different indoor resting habitats in Hubei, China [J]. Chines Journal of Vector Biology and Control, 2016, 27(1): 14-16.
[10]	XU Jun, WANG An-dong, XIAO Yun-xia, XU Xin-long, DAI Li, DU Jing-yun, WANG Li-na, ZHANG Ke, LUO Dan, MU Lu-meng, ZHANG Ya-li, XING Jian-xin, WANG Yuan-zhi. Hard tick species identification and distribution in Aibi Lake located at China-Kazakhstan border [J]. Chines Journal of Vector Biology and Control, 2016, 27(1): 21-24.
[11]	TAO Ning, SUN En-tao, ZHAN Xiao-dong, LI Chao-pin. Infestation of Neoseiulus barkeri in bedroom storage [J]. Chines Journal of Vector Biology and Control, 2016, 27(1): 25-27.
[12]	LIU De-xing, YUE Qiao-yun, LIAO Jun-lei, WEI Xiao-ya, CHEN Jian, HU Jia. DNA barcoding and morphological identification of Sarcophaga australis－a non-recorded flesh fly species in China [J]. Chines Journal of Vector Biology and Control, 2015, 26(3): 282-285.
[13]	HUA Hui-zhen, YANG Hui-yong, YUAN Xiong-feng, ZOU Bo, WANG Deng, GAO Ling-wang. Development of rat skull automatic identification system basedon random forests [J]. Chines Journal of Vector Biology and Control, 2014, 25(5): 416-420.
[14]	ZHU Zhao-min, LIN Lin, ZENG Lin-hai, ZHAO Wei, MA Ya-jun. Morphology and moleculer identification of anopheline mosquitoes in Hainan province [J]. Chines Journal of Vector Biology and Control, 2011, 22(3): 205-208,217.
[15]	LIU Hui; YAN Rong; HE Li-fang; WAN Qi-hui. Observation on hemocytes morphology of the Musca domestica larva stained by fluorescent staining methods [J]. Chines Journal of Vector Biology and Control, 2008, 19(2): 111-113.

A specimen preparation method for human Demodex

人体蠕形螨标本制作方法探讨

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments