Chines Journal of Vector Biology and Control ›› 2020, Vol. 31 ›› Issue (6): 699-701.DOI: 10.11853/j.issn.1003.8280.2020.06.015

• Investigation • Previous Articles     Next Articles

A study of colonial morphology of Yersinia pestis carrying bacteriophages

LI Cun-xiang, ZHANG Qing-wen, XIN You-quan, QI Zhi-zhen, JIN Xing, FENG Jian-ping, LI Sheng, WU Hai-lian, ZHAO Hai-hong, DAI Rui-xia   

  1. Qinghai Institute for Endemic Disease Control and Prevention, Xining 810021, Qinghai Province, China
  • Received:2020-07-23 Online:2020-12-20 Published:2020-12-20
  • Supported by:
    Supported by the Projects of Co-construction Key Laboratory for Plague Prevention and Control (No. 2019PT310003) and the Key Laboratory for Plague Prevention and Control of Qinghai Province (No. 2020-ZJ-Y23)


李存香, 张青雯, 辛有全, 祁芝珍, 金星, 冯建萍, 李胜, 吴海莲, 赵海红, 代瑞霞   

  1. 青海省地方病预防控制所鼠疫菌专业实验室, 青海 西宁 810021
  • 通讯作者: 代瑞霞,;祁芝珍,
  • 作者简介:李存香,女,主管医师,主要从事鼠疫防治工作,
  • 基金资助:

Abstract: Objective To study the colonial morphology of Yersinia pestis carrying bacteriophages and to provide a scientific basis for the accurate and rapid identification of plague pathogens. Methods Yersinia pestis carrying bacteriophages were cultured by Hottinger's agar streak method and/or antiphage serum agar method for colonial and cellular morphology observation with the naked eye and light microscope. Results Both samples contained typical Y. pestis as well as atypical Y. pestis with bacteriophages which had smaller colonies than typical strains due to the presence of bacteriophages and slow or inhibited cell growth. Conclusion Antiphage serum agar method and plate streak method allow identification of the colonial morphology of typical and atypical Y. pestis under a light microscope. These methods are simple, rapid, straightforward, and economical, and are of great significance for the identification of intractable pathogens of plague.

Key words: Yersinia pestis, Bacteriophage, Colonial morphology

摘要: 目的 研究携带鼠疫噬菌体鼠疫耶尔森菌(鼠疫菌)的菌落形态,为准确快速判定鼠疫病原体提供科学依据。方法 利用赫氏平板划线法和/或抗鼠疫噬菌体血清平板法培养携带鼠疫噬菌体的鼠疫菌,通过肉眼和光学显微镜观察鼠疫菌菌落形态和菌群形态。结果 2个样本中既含有正常鼠疫菌,又含有携带鼠疫噬菌体的菌株。显微镜下观察发现,非典型菌落因含有噬菌体较正常鼠疫菌小,其生长缓慢或者不生长。结论 经抗鼠疫噬菌体血清平板法和平板划线法,在光学显微镜下可鉴定典型鼠疫菌和非典型鼠疫菌菌落形态。该方法简单、快速、直观、经济,对鼠疫疑难病原体的鉴定工作具有重要价值。

关键词: 鼠疫耶尔森菌, 噬菌体, 菌落形态

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