Abstract: Objective Investigateon on vectors was carried out to identify the natural foci of Lyme disease in Nanyanggou forest area of Fangshan county of Lyuliang, Shanxi province. Methods Flagging and trapping methods were used to collect ticks, BSKⅡ media was used to isolate Lyme spirochetes from ticks. MLSA method was used to genotype the isolates. Results About 300 ticks were collected from Nanyanggou forest area, including Ixodes persulcatus, Haemaphysalis concinna, and Dermacentorm marginatus. Ixodes persulcatus was the predorminant species, which was accounted for 80% among the ticks. Two strains were isolated from I. persulcatus and H. concinna, respectively. MLSA analysis showed that 2 strains isolated from ticks belonged to Borrelia garinii. Conclusion There exists natural foci of Lyme disease in Lyuliang, Shanxi province. Detailed survey on people, vector and rodents should be carried out to provide data for prevention and control of Lyme disease in this area.

Key words: Lyme disease, Vector, Natural foci

摘要： 目的 对山西省吕梁市方山县南阳沟林场进行莱姆病传播媒介调查，明确是否存在莱姆病自然疫源地。方法 2015年采用布旗法在方山县南阳沟林场捕蜱，鉴定后采用BSKⅡ培养基对其进行分离培养；采用多位点序列分析（MLSA）法对分离菌株进行分型鉴定。结果 共采蜱约300只，包括全沟硬蜱、嗜群血蜱和边缘革蜱，其中全沟硬蜱为当地优势蜱种。从全沟硬蜱和嗜群血蜱中分离出2株伯氏疏螺旋体菌株，经MLSA鉴定为Borrelia garinii基因型。结论 山西省吕梁市方山县南阳沟林场为莱姆病自然疫源地。应加强对当地人群、媒介及宿主的调查，为防控莱姆病提供科学依据。

关键词: 莱姆病, 媒介, 自然疫源地