Objective To investigate the mosquitoes and arboviruses causing the Japanese encephalitis epidemic in Tumoteyou banner in Baotou of Inner Mongolia autonomous region, China in 2018. Methods The lamp trapping method was used to collect mosquito specimens in Tumoteyou banner of Baotou, and the mosquito specimens were classified and numbered based on morphological identification and were then stored in liquid nitrogen. Reverse transcription-polymerase chain reaction was used to identify mosquito viruses, and several bioinformatics softwares, including DNAStar, were used to analyze the molecular biological features of the nucleotide sequence of the virus. Results On September 8-12, 2018, a total of 208 139 mosquitoes belonging to 5 species were collected in Tumoteyou banner of Baotou, and Aedes dorsalis, Culex pipiens pallens, Cx. tritaeniorhynchus, Cx. modestus, and Ae. flavescens accounted for 46.56%, 35.77%, 8.12%, 5.25%, and 4.30%, respectively, of the mosquitoes collected. A total of 198 mosquito specimens were ground, 2 of which tested positive for flavivirus, with 1 positive for Japanese encephalitis virus (JEV) and 1 positive for Tahyna virus. Complete genome sequencing was performed for the JEV-positive specimen, and a complete genome sequence of 10 880 bp was obtained. Genotyping was performed using the PrM segment of the virus (456-695 nucleotides of the genome), and the sequence of JEV was determined as genotype I. Compared with the strain SA14-14-2 for live attenuated vaccine against Japanese encephalitis in China, the virus has a nucleotide difference of 12.59% and an amino acid difference of 2.96%. Conclusion The complete genome sequence of genotype I JEV is obtained for the first time in Ae. dorsalis in Inner Mongolia autonomous region.
[1] 李晓宇,宋宏,付士红,等. 中国流行性乙型脑炎病毒分子生物学特性研究[J]. 病毒学报,2004,20(3):200-209. DOI:10.3321/j.issn:1000-8721.2004.03.002. Li XY,Song H,Fu SH,et al. The molecular biology of Japanese encephalitis viruses isolated in China[J]. Chin J Virol,2004,20(3):200-209. DOI:10.3321/j.issn:1000-8721.2004.03.002.
[2] 王环宇,梁国栋. 我国虫媒病毒研究10年回顾[J]. 中国公共卫生,2003,19(4):473-476. DOI:10.11847/zgggws2003-19-04-51. Wang HY,Liang GD. Review of research on Arboviruses in China in the past 10 years[J]. Chin J Pub Health,2003,19(4):473-476. DOI:10.11847/zgggws2003-19-04-51.
[3] 万寒兵,杨玉荣,林亮,等. 2018年内蒙古自治区包头市一起老年人流行性乙型脑炎暴发疫情的调查[J]. 疾病监测,2019,34(12):1126-1129. DOI:10.3784/j.issn.1003-9961.2019.12.020. Wan HB,Yang YR,Lin L,et al. An outbreak of Japanese encephalitis in elderly people in Baotou,Inner Mongolia,2018[J].Dis Surveill,2019,34(12):1126-1129. DOI:10.3784/j.issn.1003-9961.2019.12.020.
[4] 程睿,南晓伟,范娜,等. 内蒙古自治区采集的蚊虫标本新发现乙脑病毒和盖塔病毒[J]. 中华流行病学杂志,2020,41(4):571-579. DOI:10.3760/cma.j.cn112338-20190425-00284. Cheng R,Nan XW,Fan N,et al. Emerging of Japanese encephalitis virus and Getah virus from specimen of mosquitoes in Inner Mongolia autonomous region[J]. Chin J Epidemiol,2020,41(4):571-579. DOI:10.3760/cma.j.cn112338-20190425-00284.
[5] Chen WR,Rico-HesseR,Tesh RB. A new genotype of Japanese encephalitis virus from Indonesia[J]. Am J Trop Med Hyg,1992,47(1):61-69. DOI:10.4269/ajtmh.1992.47.61.
[6] 曾明,俞永新,董关木,等. 乙型脑炎病毒减毒活疫苗生产株SA14-14-2基因组全序列的测定[J]. 中华微生物学和免疫学杂志,2001,21(5):69-73. DOI:10.3760/j:issn:0254-5101.2001.05.021. Zeng M,Yu YX,Dong GM,et al. Sequence analysis of the full-length genome of Japanese encephalitis virus vaccine SA14-14-2 strain[J]. Chin J Microbiol Immunol,2001,21(5):69-73. DOI:10.3760/j:issn:0254-5101.2001.05.021.
[7] 范耀春,闫真,李澄,等. 内蒙古自治区1991-2010年流行性乙型脑炎流行特征分析[J]. 中国媒介生物学及控制杂志,2012,23(2):174-175. Fan YC,Yan Z,Li C,et al. Analysis on the epidemiological characteristics of Japanese encephalitis from 1991 to 2010 in Inner Mongolia autonomous region[J]. Chin J Vector Biol Control,2012,23(2):174-175.
[8] 梁国栋. 虫媒病毒:重要的被忽略的热带传染病病原体[J]. 中国热带医学,2018,18(1):1-5. DOI:10.13604/j.cnki.46-1064/r.2018.01. Liang GD. Arbovirus:the very important pathogen of neglected tropical infectious disease in the world[J]. Chin Trop Med,2018,18(1):1-5. DOI:10.13604/j.cnki.46-1064/r.2018.01.
[9] 陆敏,孙薇,张咏适,等. 我国虫媒病毒病研究进展[J]. 中国国境卫生检疫杂志,2019,42(1):74-76. DOI:10.16408/j.1004-9770.2019.01.020. Lu M,Sun W,Zhang YS,et al. Research progress of arbovirus deseases in China[J]. Chin J Frontier Health Quarant,2019,42(1):74-76. DOI:10.16408/j.1004-9770.2019.01.020.
[10] Mohammed MAF,Galbraith SE,Radford AD,et al. Molecular phylogenetic and evolutionary analyses of Muar strain of Japanese encephalitis virus reveal it is the missing fifth genotype[J]. Infect,Gene Evol,2011,11(5):855-862. DOI:10.1016/j.meegid.2011.01.020.
[11] Li MH,Fu SH,Chen WN,et al. Molecular characterization of full-length genome of Japanese encephalitis virus genotype V isolated from Tibet,China[J]. Biomed Environ Sci,2014,27(4):231-239. DOI:10.3967/bes2014.046.
[12] Li MH,Fu SH,Chen WX,et al. Genotype V Japanese encephalitis virus is emerging[J]. PLoS Negl Trop Dis,2011,5(7):e1231. DOI:10.1371/journal.pntd.0001231.
[13] 张慧艳,高晓艳,崔世恒,等. 我国新出现的基因V型乙脑病毒全基因组分子特征[J]. 中华微生物学和免疫学杂志,2015,35(2):81-86. DOI:10.3760/cma.j.issn.0254-5101.2015.02.001. Zhang HY,Gao XY,Cui SH,et al. Whole genome sequence analysis of an emerging Japanese encephalitis virus (genotype Ⅴ) strain isolated in mainland China[J]. Chin J Microbiol Immunol,2015,35(2):81-86. DOI:10.3760/cma.j.issn.0254-5101.2015.02.001.
[14] 王环宇,付士红,李晓宇,等. 我国首次分离到基因Ⅰ型乙型脑炎病毒[J]. 中华微生物学和免疫学杂志,2004,24(11):843-849. DOI:10.3760/j:issn:0254-5101.2004.11.001. Wang HY,Fu SH,Li XY,et al. Isolation and identification of genotypeⅠJapanese encephalitis virus in China[J]. Chin J Microbiol Immunol,2004,24(11):843-849. DOI:10.3760/j:issn:0254-5101.2004.11.001.
[15] 王俊文,付士红,王环宇,等. 辽宁省乙脑病毒的分离与鉴定[J]. 中华实验和临床病毒学杂志,2006,20(1):61-65. DOI:10.3760/cma.j.issn.1003-9279.2006.01.017. Wang JW,Fu SH,Wang HY,et al. Isolation and identification of Japanese encephalitis virus in Liaoning province[J]. Chin J Exp Clin Virol,2006,20(1):61-65. DOI:10.3760/cma.j.issn.1003-9279.2006.01.017.
