内蒙古包头市2018年一起流行性乙型脑炎疫情的媒介蚊虫及感染虫媒病毒调查

doi:10.11853/j.issn.1003.8280.2020.06.005

中国媒介生物学及控制杂志 ›› 2020, Vol. 31 ›› Issue (6): 652-656.DOI: 10.11853/j.issn.1003.8280.2020.06.005

内蒙古包头市2018年一起流行性乙型脑炎疫情的媒介蚊虫及感染虫媒病毒调查

南晓伟¹, 解新霞², 于红敏³, 司晓艳¹, 陈继来¹, 张晨光¹, 张秀红¹

1 内蒙古自治区综合疾病预防控制中心病媒生物防制科, 内蒙古呼和浩特 010031;
2 乌兰察布市地方病防治中心, 内蒙古乌兰察布 012000;
3 包头医学院公共卫生学院, 内蒙古包头 014040

收稿日期:2020-06-09 出版日期:2020-12-20 发布日期:2020-12-20
通讯作者: 张晨光,Email:nmghhgyl@126.com;张秀红,Email:1097011949@qq.com
作者简介:南晓伟,女,硕士,副主任技师,长期从事传染病预防控制工作,Email:nanxw0407@163.com;解新霞,女,硕士,主任技师,长期从事鼠疫防控工作,Email:303473131@qq.com
基金资助:
内蒙古自治区科技计划项目（201802141）；国家科技重大专项（2018ZX10101002-001-009）；内蒙古自治区自然科学基金（2020MS08162）

An investigation of the vector mosquitoes and arboviruses during a Japanese encephalitis epidemic in Baotou of Inner Mongolia autonomous region, China, 2018

NAN Xiao-wei¹, XIE Xin-xia², YU Hong-min³, SI Xiao-yan¹, CHEN Ji-lai¹, ZHANG Chen-guang¹, ZHANG Xiu-hong¹

1 Inner Mongolia Autonomous Region Comprehensive Center for Disease Control and Prevention, Hohhot 010031, Inner Mongolia Autonomous Region, China;
2 Ulanqab Center for Endemic Disease Control and Prevention;
3 School of Public Health, Baotou Medical College

Received:2020-06-09 Online:2020-12-20 Published:2020-12-20
Supported by:
Supported by the Science and Technology Program of Inner Mongolia Autonomous Region (No. 201802141), National Science and Technology Major Project of China (No. 2018ZX10101002-001-009) and the Natural Science Foundation of Inner Mongolia Autonomous Region of China (No. 2020MS08162)

摘要/Abstract

摘要： 目的对2018年内蒙古自治区（内蒙古）包头市土默特右旗引发流行性乙型脑炎（乙脑）疫情的蚊虫及虫媒病毒进行调查研究。方法在包头市土默特右旗用诱蚊灯法采集蚊虫标本，形态学鉴定后分装编号，液氮保存。用反转录聚合酶链式反应（RT-PCR）检测蚊虫病毒，采用DNAStar等多种生物学信息软件对病毒核苷酸序列进行分子生物学特征分析。结果 2018年9月8－12日，在包头市土默特右旗共捕获蚊虫5种208 139只，其中背点伊蚊、淡色库蚊、三带喙库蚊、凶小库蚊、黄色伊蚊分别占捕获总数的46.56%、35.77%、8.12%、5.25%和4.30%。共研磨蚊虫标本198份，检测出2份黄病毒科阳性标本，基因测序结果显示1份为乙脑病毒（JEV）阳性，1份为Tahyna病毒阳性。对JEV阳性标本进行全基因测序，得到10 880 bp的全基因序列。利用病毒PrM区段（病毒基因组456~695位核苷酸）进行基因分型，确定JEV序列为基因Ⅰ型。与我国乙脑减毒活疫苗株SA₁₄-14-2比对，核苷酸差异为12.59%，氨基酸差异为2.96%。结论在内蒙古地区背点伊蚊中首次获得基因Ⅰ型JEV全基因组序列。

关键词: 蚊虫, 虫媒病毒, 基因型, 内蒙古自治区

Abstract: Objective To investigate the mosquitoes and arboviruses causing the Japanese encephalitis epidemic in Tumoteyou banner in Baotou of Inner Mongolia autonomous region, China in 2018. Methods The lamp trapping method was used to collect mosquito specimens in Tumoteyou banner of Baotou, and the mosquito specimens were classified and numbered based on morphological identification and were then stored in liquid nitrogen. Reverse transcription-polymerase chain reaction was used to identify mosquito viruses, and several bioinformatics softwares, including DNAStar, were used to analyze the molecular biological features of the nucleotide sequence of the virus. Results On September 8-12, 2018, a total of 208 139 mosquitoes belonging to 5 species were collected in Tumoteyou banner of Baotou, and Aedes dorsalis, Culex pipiens pallens, Cx. tritaeniorhynchus, Cx. modestus, and Ae. flavescens accounted for 46.56%, 35.77%, 8.12%, 5.25%, and 4.30%, respectively, of the mosquitoes collected. A total of 198 mosquito specimens were ground, 2 of which tested positive for flavivirus, with 1 positive for Japanese encephalitis virus (JEV) and 1 positive for Tahyna virus. Complete genome sequencing was performed for the JEV-positive specimen, and a complete genome sequence of 10 880 bp was obtained. Genotyping was performed using the PrM segment of the virus (456-695 nucleotides of the genome), and the sequence of JEV was determined as genotype I. Compared with the strain SA₁₄-14-2 for live attenuated vaccine against Japanese encephalitis in China, the virus has a nucleotide difference of 12.59% and an amino acid difference of 2.96%. Conclusion The complete genome sequence of genotype I JEV is obtained for the first time in Ae. dorsalis in Inner Mongolia autonomous region.

Key words: Mosquito, Arbovirus, Genotype, Inner Mongolia autonomous region

中图分类号:

南晓伟, 解新霞, 于红敏, 司晓艳, 陈继来, 张晨光, 张秀红. 内蒙古包头市2018年一起流行性乙型脑炎疫情的媒介蚊虫及感染虫媒病毒调查[J]. 中国媒介生物学及控制杂志, 2020, 31(6): 652-656.

NAN Xiao-wei, XIE Xin-xia, YU Hong-min, SI Xiao-yan, CHEN Ji-lai, ZHANG Chen-guang, ZHANG Xiu-hong. An investigation of the vector mosquitoes and arboviruses during a Japanese encephalitis epidemic in Baotou of Inner Mongolia autonomous region, China, 2018[J]. Chines Journal of Vector Biology and Control, 2020, 31(6): 652-656.

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内蒙古包头市2018年一起流行性乙型脑炎疫情的媒介蚊虫及感染虫媒病毒调查

An investigation of the vector mosquitoes and arboviruses during a Japanese encephalitis epidemic in Baotou of Inner Mongolia autonomous region, China, 2018

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