浙江省硬蜱科一新纪录种的形态学与分子生物学鉴定

刘营, 侯娟, 李天奇, 贯长辉, 吴雪, 吴红照, 凌锋, 张蓉, 施旭光, 任江萍, 陈恩富, 孙继民

doi:10.11853/j.issn.1003.8280.2020.02.009

中国媒介生物学及控制杂志 >

2020 , Vol. 31 >Issue 2: 164 - 168

DOI: https://doi.org/10.11853/j.issn.1003.8280.2020.02.009

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浙江省硬蜱科一新纪录种的形态学与分子生物学鉴定

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1 浙江省疾病预防控制中心传染病预防控制所, 浙江杭州 310051;
2 椒江区疾病预防控制中心, 浙江椒江 318000;
3 安吉县疾病预防控制中心, 浙江安吉 313300;
4 浦江县疾病预防控制中心, 浙江浦江 322200

刘营,男,医师,主要从事自然疫源性疾病防制工作,Email:yliu@cdc.zj.cn

收稿日期: 2019-11-11

网络出版日期: 2020-04-20

基金资助

国家自然科学基金（81872675）；浙江省医药卫生科技计划（2017KY291，2018KY338，2019KY060）

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Morphological and molecular identification of one newly recorded species of Ixodidae in Zhejiang province, China

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1 Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China;
2 Jiaojiang District Center for Disease Control and Prevention;
3 Anji County Center for Disease Control and Prevention;
4 Pujiang County Center for Disease Control and Prevention

Received date: 2019-11-11

Online published: 2020-04-20

Supported by

Supported by the National Natural Science Fundation of China (No. 81872675) and the Medical Research Program of Zhejiang Province (No. 2017KY291, 2018KY338, 2019KY060)

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摘要

目的对浙江省2018年蜱类监测过程中发现的一新纪录种进行鉴定。方法蜱标本采自浙江省椒江区、安吉和浦江县的家养动物体表及草丛、灌木丛；先以形态学鉴定，然后用PCR方法扩增得到蜱的线粒体16S rRNA基因和细胞色素C氧化酶亚基Ⅰ（COⅠ）基因，测序后进行同源性分析。结果 2018年浙江省共采集到该新纪录种13只，均为雄蜱，形态学鉴定为褐黄血蜱（Haemaphysalis flava Neumann，1897）。该蜱16S rRNA基因与已知褐黄血蜱（河南，KX450282.1）线粒体16S rRNA基因片段匹配度达100%，与其他血蜱属的同源性在86.9%～92.2%，分歧度在8.2%～14.7%之间；该蜱COⅠ基因与已知褐黄血蜱（湖南，KJ195464.1）线粒体COⅠ基因片段匹配度达99.8%，与其他血蜱属的同源性在84.7%～90.7%，分歧度在10.0%～17.2%之间。结论形态学和分子生物学鉴定方法相结合能更准确地鉴定蜱的种类。

关键词： 褐黄血蜱; 新纪录种; 分子鉴定

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刘营, 侯娟, 李天奇, 贯长辉, 吴雪, 吴红照, 凌锋, 张蓉, 施旭光, 任江萍, 陈恩富, 孙继民 . 浙江省硬蜱科一新纪录种的形态学与分子生物学鉴定[J]. 中国媒介生物学及控制杂志, 2020 , 31(2) : 164 -168 . DOI: 10.11853/j.issn.1003.8280.2020.02.009

Abstract

Objective To identify a newly recorded species discovered in tick surveillance in Zhejiang province, China, 2018. Methods Ticks were collected from grasses, shrubs, and the body surface of domestic animals in Jiaojiang district, Anji county, and Pujiang county of Zhejiang province, China. Morphological identification was conducted, followed by amplification of the mitochondrial 16S rRNA and cytochrome coxidase I (COⅠ) genes by PCR. After sequencing, homology analyses were performed. Results In 2018, 13 male ticks, a newly recorded species captured in Zhejiang province, were morphologically identified as Haemaphysalis flava Neumann (1897). The mitochondrial 16S rRNA gene of this tick displayed 100% identity to the known fragment of that gene of H. flava (Henan, KX450282.1); in comparison with other Haemaphysalis, the homology was 86.9%-92.2% and the divergence was 8.2%-14.7%. The mitochondrial COⅠgene of this tick showed 99.8% identity to the known COⅠ gene fragment of H. flava (Hunan, KJ195464.1); in comparison with other Haemaphysalis, the homology was 84.7%-90.7% and the divergence was 10.0%-17.2%. Conclusion The combination of morphological and molecular biological identification methods can accurately identify the species of ticks.

Key words： Haemaphysalis flava; Newly recorded species; Molecular identification

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