浙江省硬蜱科一新纪录种的形态学与分子生物学鉴定

doi:10.11853/j.issn.1003.8280.2020.02.009

中国媒介生物学及控制杂志 ›› 2020, Vol. 31 ›› Issue (2): 164-168.DOI: 10.11853/j.issn.1003.8280.2020.02.009

浙江省硬蜱科一新纪录种的形态学与分子生物学鉴定

刘营¹, 侯娟¹, 李天奇¹, 贯长辉², 吴雪³, 吴红照⁴, 凌锋¹, 张蓉¹, 施旭光¹, 任江萍¹, 陈恩富¹, 孙继民¹

1 浙江省疾病预防控制中心传染病预防控制所, 浙江杭州 310051;
2 椒江区疾病预防控制中心, 浙江椒江 318000;
3 安吉县疾病预防控制中心, 浙江安吉 313300;
4 浦江县疾病预防控制中心, 浙江浦江 322200

收稿日期:2019-11-11 出版日期:2020-04-20 发布日期:2020-04-20
通讯作者: 孙继民,Email:jmsun@cdc.zj.cn
作者简介:刘营,男,医师,主要从事自然疫源性疾病防制工作,Email:yliu@cdc.zj.cn
基金资助:
国家自然科学基金（81872675）；浙江省医药卫生科技计划（2017KY291，2018KY338，2019KY060）

Morphological and molecular identification of one newly recorded species of Ixodidae in Zhejiang province, China

LIU Ying¹, HOU Juan¹, LI Tian-qi¹, GUAN Chang-hui², WU Xue³, WU Hong-zhao⁴, LING Feng¹, ZHANG Rong¹, SHI Xu-guang¹, REN Jiang-ping¹, CHEN En-fu¹, SUN Ji-min¹

1 Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China;
2 Jiaojiang District Center for Disease Control and Prevention;
3 Anji County Center for Disease Control and Prevention;
4 Pujiang County Center for Disease Control and Prevention

Received:2019-11-11 Online:2020-04-20 Published:2020-04-20
Supported by:
Supported by the National Natural Science Fundation of China (No. 81872675) and the Medical Research Program of Zhejiang Province (No. 2017KY291, 2018KY338, 2019KY060)

摘要/Abstract

摘要： 目的对浙江省2018年蜱类监测过程中发现的一新纪录种进行鉴定。方法蜱标本采自浙江省椒江区、安吉和浦江县的家养动物体表及草丛、灌木丛；先以形态学鉴定，然后用PCR方法扩增得到蜱的线粒体16S rRNA基因和细胞色素C氧化酶亚基Ⅰ（COⅠ）基因，测序后进行同源性分析。结果 2018年浙江省共采集到该新纪录种13只，均为雄蜱，形态学鉴定为褐黄血蜱（Haemaphysalis flava Neumann，1897）。该蜱16S rRNA基因与已知褐黄血蜱（河南，KX450282.1）线粒体16S rRNA基因片段匹配度达100%，与其他血蜱属的同源性在86.9%～92.2%，分歧度在8.2%～14.7%之间；该蜱COⅠ基因与已知褐黄血蜱（湖南，KJ195464.1）线粒体COⅠ基因片段匹配度达99.8%，与其他血蜱属的同源性在84.7%～90.7%，分歧度在10.0%～17.2%之间。结论形态学和分子生物学鉴定方法相结合能更准确地鉴定蜱的种类。

关键词: 褐黄血蜱, 新纪录种, 分子鉴定

Abstract: Objective To identify a newly recorded species discovered in tick surveillance in Zhejiang province, China, 2018. Methods Ticks were collected from grasses, shrubs, and the body surface of domestic animals in Jiaojiang district, Anji county, and Pujiang county of Zhejiang province, China. Morphological identification was conducted, followed by amplification of the mitochondrial 16S rRNA and cytochrome coxidase I (COⅠ) genes by PCR. After sequencing, homology analyses were performed. Results In 2018, 13 male ticks, a newly recorded species captured in Zhejiang province, were morphologically identified as Haemaphysalis flava Neumann (1897). The mitochondrial 16S rRNA gene of this tick displayed 100% identity to the known fragment of that gene of H. flava (Henan, KX450282.1); in comparison with other Haemaphysalis, the homology was 86.9%-92.2% and the divergence was 8.2%-14.7%. The mitochondrial COⅠgene of this tick showed 99.8% identity to the known COⅠ gene fragment of H. flava (Hunan, KJ195464.1); in comparison with other Haemaphysalis, the homology was 84.7%-90.7% and the divergence was 10.0%-17.2%. Conclusion The combination of morphological and molecular biological identification methods can accurately identify the species of ticks.

Key words: Haemaphysalis flava, Newly recorded species, Molecular identification

中图分类号:

R384.4

刘营, 侯娟, 李天奇, 贯长辉, 吴雪, 吴红照, 凌锋, 张蓉, 施旭光, 任江萍, 陈恩富, 孙继民. 浙江省硬蜱科一新纪录种的形态学与分子生物学鉴定[J]. 中国媒介生物学及控制杂志, 2020, 31(2): 164-168.

LIU Ying, HOU Juan, LI Tian-qi, GUAN Chang-hui, WU Xue, WU Hong-zhao, LING Feng, ZHANG Rong, SHI Xu-guang, REN Jiang-ping, CHEN En-fu, SUN Ji-min. Morphological and molecular identification of one newly recorded species of Ixodidae in Zhejiang province, China[J]. Chines Journal of Vector Biology and Control, 2020, 31(2): 164-168.

参考文献

[1] 陈泽. 中国蜱类的系统分类及两种硬蜱的生物学特性分析[D]. 石家庄:河北师范大学,2010. Chen Z. Taxonomic and systematic research of Chinese ticks and biological characteristic analysis of two hard tick species[D]. Shijiazhuang:Hebei Normal University,2010.
[2] 王斐. 蜱媒传染病的流行特征及防控策略[J]. 热带病与寄生虫学,2016,14(1):54-56,50.DOI:10.3969/j.issn.1672-2302. 2016.01.020. Wang F. Epidemiological characteristics and prevention strategies of vector-borne diseases[J]. J Trop Dis Parasitol,2016,14(1):54-56,50. DOI:10.3969/j.issn.1672-2302.2016. 01.020.
[3] Zhuang L,Sun Y,Cui XM,et al. Transmission of severe fever with thrombocytopenia syndrome virus by Haemaphysalis longicornis ticks,China[J]. Emerg Infect Dis,2018,24(5):868-871. DOI:10.3201/eid2405.151435.
[4] 邓国藩,姜在阶. 中国经济昆虫志. 第39册.硬蜱科[M]. 北京:科学出版社,1991:1-43. Deng GF,Jiang ZJ. Economic insect fauna of China Fasc 39(Acari:Ixodadae) Volume 39[M]. Beijing:Science Press,1991:1-43.
[5] Chen Z,Li YQ,Ren QY,et al. Dermacentor everestianus Hirst,1926(Acari:Ixodidae):phylogenetic status inferred from molecular characteristics[J]. Parasitol Res,2014,113(10):3773-3779.
[6] 王强,何宇平,吴成云,等. 上海口岸1只进境犬携带蜱的种类鉴定与溯源分析[J]. 中国媒介生物学及控制杂志,2018,29(4):340-343. DOI:10.11853/j.issn.1003.8280.2018.04.004. Wang Q,He YP,Wu CY,et al. Identification and traceability of an intercepted tick from imported dog at Shanghai port[J]. Chin J Vector Biol Control,2018,29(4):340-343. DOI:10.11853/j.issn.1003.8280.2018.04.004.
[7] Folmer O,Black M,Hoeh W,et al. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol,1994,3(5):294-299.
[8] 杨鹏飞,燕清丽,苏琦,等. 基于mt COⅠ基因序列的DNA条形码技术在江苏沿江长角血蜱鉴定中的应用[J]. 江苏预防医学,2018,29(2):135-139. DOI:10.13668/j.issn.1006-9070.2018. 02.006. Yang PF,Yan QL,Su Q,et al. Application of DNA barcoding technolodge based on the mitochondrial gene COⅠ to identify Haemaphysalis longicornis along the Yangtse River in Jiangsu province[J]. Jiangsu J Prevent Med,2018,29(2):135-139. DOI:10.13668/j.issn.1006-9070.2018.02.006.
[9] 许红彬,黄鹤,许忠济,等. 江西省硬蜱科6新纪录种[J]. 华中师范大学学报:自然科学版,2017,51(6):804-808. DOI:10.19603/j.cnki.1000-1190.2017.06.014. Xu HB,Huang H,Xu ZJ,et al. Six newly recorded species of Ixodidae in Jiangxi province,China[J]. J Central China Normal Univ:Nat Sci,2017,51(6):804-808. DOI:10.19603/j.cnki. 1000-1190.2017.06.014.
[10] 宋金秋,李晖,谭磊,等. 基于cox1基因序列分析山羊褐黄血蜱种系发育关系[J]. 中国预防兽医学报,2017,39(7):594-596. DOI:10.3969/j.issn.1008-0425.2017.07.17. Song JQ,Li H,Tan L,et al. Phylogenetic analysis of mitochondrial cox1 genes for Haemaphysalis flava from goats[J]. Chin J Prevent Vet Med,2017,39(7):594-596. DOI:10.3969/j.issn.1008-0425.2017.07.17.
[11] 严芬,程天印. 褐黄血蜱的形态学与分子生物学鉴定[J]. 中国兽医学报,2015,35(6):912-916. DOI:10.16303/j.cnki.1005-4545.2015.06.13. Yan F,Cheng TY. Morphological and molecular identification of Haemaphysalis flava[J]. Chin J Vet Sci,2015,35(6):912-916. DOI:10.16303/j.cnki.1005-4545.2015.06.13.
[12] Choi CY,Kang CW,Kim EM,et al. Ticks collected from migratory birds,including a new record of Haemaphysalis formosensis,on Jeju Island,Korea[J]. Exp Appl Acarol,2014,62(4):557-566. DOI:10.1007/s10493-013-9748-9.
[13] Kim BJ,Kim H,Won S,et al. Ticks collected from wild and domestic animals and natural habitats in the Republic of Korea[J]. Korean J Parasitol,2014,52(3):281-285. DOI:10.3347/kjp.2014.52.3.281.
[14] Lv JZ,Wu SQ,Zhang YN,et al. Assessment of four DNA fragments (COI,16S rDNA,ITS2,12S rDNA) for species identification of the Ixodida (Acari:Ixodida)[J]. Parasit Vect,2014,7(1):93. DOI:10.1186/1756-3305-7-93.
[15] Chen Z,Yang XJ,Bu FJ,et al. Morphological,biological and molecular characteristics of bisexual and parthenogenetic Haemaphysalis longicornis[J]. Vet Parasitol,2012,189(2/4):344-352. DOI:10.1016/j.vetpar.2012.04.021.
[16] Black Ⅳ WC,Piesman J. Phylogeny of hard-and soft-tick taxa (Acari:Ixodida) based on mitochondrial 16S rDNA sequences[J]. Proc Natl Acad Sci USA,1994,91(21):10034-10038. DOI:10.1073/pnas.91.21.10034.
[17] 侯海燕. COXⅠ基因DNA条形码技术在蜱类鉴别中的应用[J]. 国际检验医学杂志,2015,36(24):3570-3571. DOI:10.3969/j.issn.1673-4130.2015.24.026. Hou HY. Application of DNA barcoding technique of COXⅠgene in identification of ticks[J]. Int J Lab Med,2015,36(24):3570-3571. DOI:10.3969/j.issn.1673-4130.2015.24.026.
[18] 江莉,龚劲聪,胡龙飞,等. DNA条形码技术用于进口货物中肩突硬蜱的鉴定[J]. 中国国境卫生检疫杂志,2014,37(1):33-36. DOI:10.16408/j.1004-9770.2014.01.007. Jiang L,Gong JC,Hu LF,et al. Application of DNA barcode technology for detection of Ixodes scapularis from imported goods[J]. Chin J Front Health Quarant,2014,37(1):33-36. DOI:10.16408/j.1004-9770.2014.01.007.
[19] 覃新程,田俊华,王剑波,等. 长角血蜱和微小扇头蜱的形态与分子生物学鉴定[J]. 中华流行病学杂志,2011,32(6):608-612. DOI:10.3760/cma.j.issn.0254-6450.2011.06.017. Qin XC,Tian JH,Wang JB,et al. Identification of Haemaphysalis longicomis and Rhipicephalus microplus[J]. Chin J Epidemiol,2011,32(6):608-612. DOI:10.3760/cma.j.issn.0254-6450. 2011.06.017.
[20] 马英,鲁亮. DNA条形码技术[J]. 中国媒介生物学及控制杂志,2012,23(3):185-190,205. Ma Y,Lu L. DNA barcode technology[J]. Chin J Vector Biol Control,2012,23(3):185-190,205.

浙江省硬蜱科一新纪录种的形态学与分子生物学鉴定

Morphological and molecular identification of one newly recorded species of Ixodidae in Zhejiang province, China

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李心玫, 孙梦涛, 詹雨娟, 张兰香, 李梦珠, 陶东东, 孙恩涛. 马六甲肉食螨与转开肉食螨的形态及分子鉴定[J]. 中国媒介生物学及控制杂志, 2021, 32(2): 230-234.
[2]	郑凌霄, 尹灿灿, 王逸枭, 李梦楠, 邵黄芳, 方惟希, 杨曼, 赵金红, 孙恩涛. 基于DNA条形码技术的粉螨种类鉴定研究[J]. 中国媒介生物学及控制杂志, 2019, 30(2): 180-184.
[3]	刘德星, 邱德义, 李婷婷, 张瑞玲, 刘春晓, 岳巧云. 热带臭虫及温带臭虫形态与分子鉴定[J]. 中国媒介生物学及控制杂志, 2018, 29(6): 576-581.
[4]	周淑姮, 温廷桓, 邓艳琴, 林代华, 肖方震, 徐国英. 中国真厉螨属区系分类研究及一新纪录种记述[J]. 中国媒介生物学及控制杂志, 2018, 29(5): 482-487.
[5]	王强, 何宇平, 吴成云, 王巧全, 王艳, 林颖峥, 陈志飞, 张强, 邓耀华, 张冠楠, 曹宇凡, 唐智芳, 李树清. 上海口岸1只进境犬携带蜱的种类鉴定与溯源分析[J]. 中国媒介生物学及控制杂志, 2018, 29(4): 340-343.
[6]	陈红娜, 褚宏亮, 刘大鹏, 周明浩. 我国室内常见蜚蠊的分子鉴定研究[J]. 中国媒介生物学及控制杂志, 2018, 29(1): 20-22.
[7]	段琛, 韩晓静, 常琼琼, 侯晓晖. 广西壮族自治区大瑶山国家级自然保护区蠓类分布调查[J]. 中国媒介生物学及控制杂志, 2018, 29(1): 73-75.
[8]	邱德义, 魏晓雅, 吴可量, 岳巧云. 德国小蠊及其近缘种的形态和分子鉴定[J]. 中国媒介生物学及控制杂志, 2017, 28(3): 220-225.
[9]	刘宁, 王明福. 中国厕蝇属明厕蝇种团一新纪录种(双翅目:厕蝇科)[J]. 中国媒介生物学及控制杂志, 2016, 27(5): 494-495.
[10]	许红彬, 许忠济, 黄鹤, 段小兰, 李育强, 陈克非, 王琪, 吴亚云. 江西省蜱类二新纪录种(寄螨目:硬蜱科)[J]. 中国媒介生物学及控制杂志, 2016, 27(5): 496-497.
[11]	张勤, 王晓梦, 岳巧云, 邱德义, 高洁, 张芳, 王凯. 河南口岸截获未知蝇种细胞色素C氧化酶亚基Ⅰ基因分析[J]. 中国媒介生物学及控制杂志, 2016, 27(4): 354-357.
[12]	张勤, 王晓梦, 岳巧云, 高洁, 邱德义. 河南口岸截获鼠类DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2016, 27(3): 280-282.
[13]	田隽, 王明福, 王晶. 无斑厕蝇中国新纪录种(双翅目:厕蝇科)[J]. 中国媒介生物学及控制杂志, 2016, 27(1): 42-43.
[14]	李鑫, 王明福, 王晶. 中国厕蝇属一新纪录种(双翅目:厕蝇科)[J]. 中国媒介生物学及控制杂志, 2015, 26(6): 609-610.
[15]	杜景云, 牟路萌, 张璘, 王丽娜, 王安东, 张科, 陈创夫, 王远志. 新疆边境地区伊宁县图兰扇头蜱形态学及分子生物学鉴定[J]. 中国媒介生物学及控制杂志, 2015, 26(3): 271-274.