目的 调查浙江省衢州市1例登革热输入性病例流行病学特征及病原体分子溯源，为登革热的防控提供依据。方法 对2017年输入性登革热病例进行流行病学调查，采集病例血清进行登革热病毒核酸和抗体检测，提取病毒核酸后扩增E基因并测序，利用生物信息学软件进行多序列比对并构建进化树。结果 该病例登革热病毒核酸及IgM抗体检测结果均为阳性，基因序列比对及进化分析，病毒株为登革热病毒1型Ⅰ亚型，与东南亚病毒株（登录号KY586429.1、KJ806941.2）亲缘关系最近，核苷酸和氨基酸同源性分别为99.1%和99.7%。结论 衢州市登革热病毒1型病例可能为来自东南亚的1例输入性病例。
Objective To understand the pathogen's epidemiological origination and molecular characteristics of the dengue fever case in Quzhou city, Zhejiang. Methods The epidemiological survey was conducted and the serum sample was collected from the imported dengue fever patient in 2017 to detect dengue virus nucleic acids and antibody. The envelope (E) gene of the virus was amplified and sequenced. The genotype of the isolate was analyzed with bioinformatics software. Results The serum sample of the patient was positive for dengue virus nucleic and anti-dengue IgM. The homologous and phylogenetic analysis showed that the isolate belonged to dengue virus type 1, and the sub genotype was GI. It had a closest phylogenetic relationship with the strain isolated from the case of Southeast Asia (accession number KY586429.1, KJ806941.2), sharing 99.1% and 99.7% homology in nucleotide sequence and amino acid sequence respectively, indicating that it might have originated from the Southeast Asia. Conclusion The imported dengue fever case in Quzhou city might be contracted from the Southeast Asia.
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