中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (5): 445-447.DOI: 10.11853/j.issn.1003.8280.2018.05.006

• 论著 • 上一篇    下一篇

浙江省衢州市1例输入性登革热病例分子流行病学研究

杨瑞军1, 黄世腾1, 王晓光2, 吕磊1, 曹国平1, 万圣1, 叶承华1, 陈旭富1   

  1. 1 衢州市疾病预防控制中心, 浙江 衢州 324000;
    2 丽水市疾病预防控制中心, 浙江 丽水 323000
  • 收稿日期:2018-05-30 出版日期:2018-10-20 发布日期:2018-10-20
  • 作者简介:杨瑞军,男,副主任技师,主要从事病原微生物学研究,Email:qzyangruijun@126.com

Molecular epidemiological studies on an imported dengue fever case in Quzhou city, Zhejiang

YANG Rui-jun1, HUANG Shi-teng1, WANG Xiao-guang2, LYU Lei1, CAO Guo-ping1, WAN Sheng1, YE Cheng-hua1, CHEN Xu-fu1   

  1. 1 Quzhou Center for Disease Control and Prevention, Quzhou 324000, Zhejiang Province, China;
    2 Lishui Center for Disease Control and Prevention
  • Received:2018-05-30 Online:2018-10-20 Published:2018-10-20

摘要: 目的 调查浙江省衢州市1例登革热输入性病例流行病学特征及病原体分子溯源,为登革热的防控提供依据。方法 对2017年输入性登革热病例进行流行病学调查,采集病例血清进行登革热病毒核酸和抗体检测,提取病毒核酸后扩增E基因并测序,利用生物信息学软件进行多序列比对并构建进化树。结果 该病例登革热病毒核酸及IgM抗体检测结果均为阳性,基因序列比对及进化分析,病毒株为登革热病毒1型Ⅰ亚型,与东南亚病毒株(登录号KY586429.1、KJ806941.2)亲缘关系最近,核苷酸和氨基酸同源性分别为99.1%和99.7%。结论 衢州市登革热病毒1型病例可能为来自东南亚的1例输入性病例。

关键词: 输入性病例, 登革热, 登革热病毒, E基因, 序列分析, 生物信息学

Abstract: Objective To understand the pathogen's epidemiological origination and molecular characteristics of the dengue fever case in Quzhou city, Zhejiang. Methods The epidemiological survey was conducted and the serum sample was collected from the imported dengue fever patient in 2017 to detect dengue virus nucleic acids and antibody. The envelope (E) gene of the virus was amplified and sequenced. The genotype of the isolate was analyzed with bioinformatics software. Results The serum sample of the patient was positive for dengue virus nucleic and anti-dengue IgM. The homologous and phylogenetic analysis showed that the isolate belonged to dengue virus type 1, and the sub genotype was GI. It had a closest phylogenetic relationship with the strain isolated from the case of Southeast Asia (accession number KY586429.1, KJ806941.2), sharing 99.1% and 99.7% homology in nucleotide sequence and amino acid sequence respectively, indicating that it might have originated from the Southeast Asia. Conclusion The imported dengue fever case in Quzhou city might be contracted from the Southeast Asia.

Key words: Imported case, Dengue fever, Dengue virus, Envelope gene, Sequence analysis, Bioinformatics

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