目的 建立高通量半自动化的细菌传代体系。方法 使用自动液体处理器、可丢弃性机械手枪头以及带有二维码的顶旋冻存管建立高通量半自动化的细菌传代体系,比较不同参数下构建体系细菌的交叉污染率。结果 原始菌液与新鲜细菌储存液的混匀体积、活塞移动速度、可丢弃型机械手枪头及液面间的位置关系与菌种交叉污染相关;高通量半自动化的细菌传代体系效率高于手工方法。结论 高通量半自动化的细菌传代体系可提高细菌保存的工作效率,简化了菌种保藏实验室的工作流程,具有较好的应用前景。

Objective To establish high throughput semi-automated bacteria passage system. Methods Perkin Elmer automated workstation, 2-D barcode top screw frozen tubes, and disposable tips were used to establish semi-automated bacteria passage system. The recovery rate was determined and the cross-contamination of the system was compared with different parameters. Results The recovery rate depended on the times of mix and the volume of broth media. The cross-contamination was related to the volume of mixture, motion speed of the plunger and the position relationship between the disposable tips and liquid surface. Compared with manual method, this semi-automated system greatly improved the efficiency. Conclusion A high throughput semi-automated bacteria passage system was established. This system could be integrated into the routine workflow of professional bacteria culture collection lab.