黑龙江省逊克县火山熔岩地区啮齿动物携带病原体调查

杨军, 鞠文东, 焦丹, 徐宁, 梁慧杰, 程成, 王晓杰, 付维明, 王艳梅, 耿聪

doi:10.11853/j.issn.1003.4692.2015.03.005

中国媒介生物学及控制杂志 >

2015 , Vol. 26 >Issue 3: 238 - 241

DOI: https://doi.org/10.11853/j.issn.1003.4692.2015.03.005

论著

黑龙江省逊克县火山熔岩地区啮齿动物携带病原体调查

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1 逊克出入境检验检疫局, 黑龙江逊克164400;
2 黑龙江省国际旅行卫生保健中心;
3 黑河出入境检验检疫局;
4 萝北出入境检验检疫局;
5 黑龙江省皮肤病防治研究所

杨军,男,副主任技师,主要从事国境卫生检疫工作,Email: yangjun4453194@163.com

收稿日期: 2015-02-27

网络出版日期: 2015-06-20

基金资助

国家质量监督检验检疫总局科研基金(2014IK047);黑龙江出入境检验检疫局科技计划项目(2014HK007)

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Investigation on rodent-borne pathogens in volcano lava areas of Xunke county, Heilongjiang, China

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1 Xunke Entry-Exit Inspection and Quarantine Bureau, Xunke 164400, Heilongjiang Province, China;
2 Heilongjiang International Travel Healthcare Center;
3 Heihe Entry-Exit Inspection and Quarantine Bureau;
4 Luobei Entry-Exit Inspection and Quarantine Bureau;
5 Heilongjiang Institute of Dermatology Prevention

Received date: 2015-02-27

Online published: 2015-06-20

Supported by

Supported by the General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China Scientific Research Subject(No. 2014IK047)and the Heilongjiang Import and Export Inspection and Quarantine Bureau autonomous Scientific Research Subject(No. 2014HK007)

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摘要

目的调查黑龙江省逊克县火山熔岩地区啮齿动物携带病原体状况。方法 2014年4－9月,采集啮齿动物样本107只,解剖取脏器肺、肾、肝、肾和膀胱样本,采用PCR方法分别扩增10种鼠传病原体的特异性核酸片段,通过基因测序进一步鉴定。结果在107份鼠肺中共检出汉城型汉坦病毒RNA核酸阳性样本5份,感染率为4.67%;107份鼠肾中共检出钩端螺旋体DNA核酸阳性样本6份,感染率为5.61%;107份鼠脾中共检出巴尔通体DNA核酸阳性样本7份,感染率为6.54%,嗜吞噬细胞无形体4份,感染率为3.74%;107份鼠肝、鼠脾中鼠疫菌、巴贝西原虫、斑点热群立克次体、恙虫病立克次体、莫氏立克次体的DNA核酸检测结果均为阴性;107份鼠膀胱中伯氏疏螺旋体的DNA核酸检测结果为阴性;在鼠体内还存在复合感染情况,感染率为2.80%。结论黑龙江省逊克县火山熔岩地区啮齿动物中存在汉坦病毒、钩端螺旋体、巴尔通体和嗜吞噬细胞无形体的感染。

关键词： 啮齿动物; 鼠传病原体; 聚合酶链反应检测; 黑龙江省逊克县; 火山熔岩地区

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杨军, 鞠文东, 焦丹, 徐宁, 梁慧杰, 程成, 王晓杰, 付维明, 王艳梅, 耿聪 . 黑龙江省逊克县火山熔岩地区啮齿动物携带病原体调查[J]. 中国媒介生物学及控制杂志, 2015 , 26(3) : 238 -241 . DOI: 10.11853/j.issn.1003.4692.2015.03.005

Abstract

Objective To investigate rodent-borne pathogens in volcano lava areas of Xunke county in Heilongjiang, China. Methods A total of 107 rodents were collected during April to September in 2014, and necropsy was conducted to acquire lung, kidney, liver, and kidney and bladder samples. All samples were amplified for the specific fragments of 10 rodent - borne pathogens by PCR, and further identified by gene sequencing. Results Five positive samples of SEO Hantavirus RNA were detected from 107 rodent's lung tissues, infection prevalence was 4.67%; 6 positive samples of leptospirosis DNA were detected from 107 rodent's kidney tissues, infection prevalence was 5.61%; 7 positive samples of Bartonella and 4 positive samples of Anaplasma phagocytophilum were detected from 107 rodent's spleen tissues, the prevalence of infection were 6.54% and 3.74% respectively; Detection of Yersinia pestis, Babesia, Spotted fever group rickettsia, Rickettsia tsutsugamushi, Rickettsia mooseri from rodent's liver and spleen tissues and Borrelia burgdorferi from rodent's Bladder was all negative. Multiple infection was found in some rodents, the infection prevalence was 2.80%. Conclusion Hantavirus, Leptospirosis, Bartonella, Anaplasma phagocytophilum exist in rodent populations that dwell in the volcano lava areas of Xunke county, Heilongjiang, China.

Key words： Rodents; Rodent-borne pathogen; Polymerase chain reaction measuring; Heilongjiang Xunke county; Lava areas

参考文献

[1] 路景龙. 红星火山岩地质公园晋升省级公园占地1685平方公里[EB/OL].(2008-11-24)[2015-02-25]. http://heilongjiang. dbw.cn/system/2008/11/24/051617106.shtml.
[2] 郑剑宁,王燕,裘炯良. 鼠传疾病与鼠类宿主研究概况[J]. 中国媒介生物学及控制杂志,2007,18(5):427-429.
[3] 鲍毅新. 人类的大敌:老鼠[J]. 大自然,1984(1):42-43.
[4] 王勇,张美文,李波. 鼠类防治实用技术手册[M]. 北京:金盾出版社,2003:9-17.
[5] 曹晓梅,王静,孙肖红,等. 中俄双边7个口岸地区鼠传疾病病原体调查[J]. 寄生虫与医学昆虫学报,2011,18(3):184-188.
[6] 鞠文东. SN/T 3392-2012 国境口岸莱姆病螺旋体检验规程[S]. 北京:中国标准出版社,2012.
[7] Beelitz P,Schumacher S,Marholdt F,et al. The prevalence of Babesia canis canis in marsh ticks(Dermacentor reticulatus) in Saarland[J]. Berl Munch Tierarztl Wochenschr,2012,125(3/ 4):168-171.
[8] Brouqui P,Bacellar F,Baranton G,et al. Guidelines for the diagnosis of tick - borne bacterial diseases in Europe[J]. Clin Microbiol Infect,2004,10(12):1108-1132.
[9] Otranto D,Dantas-Torres F,Giannelli A,et al. Ticks infesting humans in Italy and associated pathogens[J]. Parasit Vectors, 2014,7(1):328.
[10] 宋锋林,王静,张晓龙,等. 蜱类携带粒细胞无形体的核酸检测及核酸序列分析[J]. 中国国境卫生检疫杂志,2011,34 (5):348-350.
[11] 张倩. 内蒙古、新疆地区恙虫病流行病学调查研究[D]. 北京: 中国人民解放军军事医学科学院,2007.
[12] 杨军,关承春,温占清,等. 逊克口岸鼠类本底调查报告[J]. 中国国境卫生检疫杂志,2006,29(6):350-351.
[13] 宋干. 新中国流行性出血热防治研究的主要成就[J]. 中华流行病学杂志,2000,21(5):378-382.
[14] 陈淑红,彭永刚,陈露菲,等. 褐家鼠汉坦病毒黑龙江SC106分离株S基因特征分析[J]. 中国预防兽医学报,2010,32(8): 638-640.
[15] 翁景清,谢荣辉,卢亦愚,等. 汉坦病毒东方田鼠分离株ZT10 的M 基因序列的特性分析[J]. 中国病毒学,2006,21(3): 235-237.
[16] 任清明,刘国平,杨国平,等. 黑龙江省中俄边境地区鼠类的调查研究[J]. 中国媒介生物学及控制杂志,2009,20(3): 219-220.
[17] 张晓龙,赵婷婷,魏莲,等. 2009年中俄边境黑龙江四口岸啮齿动物及其感染病原调查研究[J]. 中国国境卫生检疫杂志, 2010,33(6):387-390.
[18] 白瑛,Kosoy MY,Maupin GO,等. 首次证实巴尔通体在我国云南鼠群中流行[J]. 中国人兽共患病杂志,2002,18(3):5-9.
[19] 王振东,王莎莎,刘丽娟,等. 2011年黑龙江省黑瞎子岛鼠群中钩端螺旋体感染状况调查[J]. 中华预防医学杂志,2013, 47(6):510-513.
[20] 左双燕. 我国黑龙江林区鼠形动物巴尔通体感染调查与分离鉴定[D]. 长沙:中南大学,2012.
[21] 唐琨. 黑龙江林区蜱和鼠中3种病原的携带状况研究[D]. 长沙:中南大学,2012.

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