中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (3): 238-241.DOI: 10.11853/j.issn.1003.4692.2015.03.005

• 论著 • 上一篇    下一篇

黑龙江省逊克县火山熔岩地区啮齿动物携带病原体调查

杨军1, 鞠文东2, 焦丹3, 徐宁2, 梁慧杰4, 程成2, 王晓杰5, 付维明2, 王艳梅2, 耿聪2   

  1. 1 逊克出入境检验检疫局, 黑龙江逊克164400;
    2 黑龙江省国际旅行卫生保健中心;
    3 黑河出入境检验检疫局;
    4 萝北出入境检验检疫局;
    5 黑龙江省皮肤病防治研究所
  • 收稿日期:2015-02-27 出版日期:2015-06-20 发布日期:2015-06-20
  • 作者简介:杨军,男,副主任技师,主要从事国境卫生检疫工作,Email: yangjun4453194@163.com
  • 基金资助:

    国家质量监督检验检疫总局科研基金(2014IK047);黑龙江出入境检验检疫局科技计划项目(2014HK007)

Investigation on rodent-borne pathogens in volcano lava areas of Xunke county, Heilongjiang, China

YANG Jun1, JU Wen-dong2, JIAO Dan3, XU Ning2, LIANG Hui-jie4, CHENG Cheng2, WANG Xiao-jie5, FU Wei-ming2, WANG Yan-mei2, GENG Cong2   

  1. 1 Xunke Entry-Exit Inspection and Quarantine Bureau, Xunke 164400, Heilongjiang Province, China;
    2 Heilongjiang International Travel Healthcare Center;
    3 Heihe Entry-Exit Inspection and Quarantine Bureau;
    4 Luobei Entry-Exit Inspection and Quarantine Bureau;
    5 Heilongjiang Institute of Dermatology Prevention
  • Received:2015-02-27 Online:2015-06-20 Published:2015-06-20
  • Supported by:

    Supported by the General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China Scientific Research Subject(No. 2014IK047)and the Heilongjiang Import and Export Inspection and Quarantine Bureau autonomous Scientific Research Subject(No. 2014HK007)

摘要:

目的 调查黑龙江省逊克县火山熔岩地区啮齿动物携带病原体状况。方法 2014年4-9月,采集啮齿动物样本107只,解剖取脏器肺、肾、肝、肾和膀胱样本,采用PCR方法分别扩增10种鼠传病原体的特异性核酸片段,通过基因测序进一步鉴定。结果 在107份鼠肺中共检出汉城型汉坦病毒RNA核酸阳性样本5份,感染率为4.67%;107份鼠肾中共检出钩端螺旋体DNA核酸阳性样本6份,感染率为5.61%;107份鼠脾中共检出巴尔通体DNA核酸阳性样本7份,感染率为6.54%,嗜吞噬细胞无形体4份,感染率为3.74%;107份鼠肝、鼠脾中鼠疫菌、巴贝西原虫、斑点热群立克次体、恙虫病立克次体、莫氏立克次体的DNA核酸检测结果均为阴性;107份鼠膀胱中伯氏疏螺旋体的DNA核酸检测结果为阴性;在鼠体内还存在复合感染情况,感染率为2.80%。结论 黑龙江省逊克县火山熔岩地区啮齿动物中存在汉坦病毒、钩端螺旋体、巴尔通体和嗜吞噬细胞无形体的感染。

关键词: 啮齿动物, 鼠传病原体, 聚合酶链反应检测, 黑龙江省逊克县, 火山熔岩地区

Abstract:

Objective To investigate rodent-borne pathogens in volcano lava areas of Xunke county in Heilongjiang, China. Methods A total of 107 rodents were collected during April to September in 2014, and necropsy was conducted to acquire lung, kidney, liver, and kidney and bladder samples. All samples were amplified for the specific fragments of 10 rodent - borne pathogens by PCR, and further identified by gene sequencing. Results Five positive samples of SEO Hantavirus RNA were detected from 107 rodent's lung tissues, infection prevalence was 4.67%; 6 positive samples of leptospirosis DNA were detected from 107 rodent's kidney tissues, infection prevalence was 5.61%; 7 positive samples of Bartonella and 4 positive samples of Anaplasma phagocytophilum were detected from 107 rodent's spleen tissues, the prevalence of infection were 6.54% and 3.74% respectively; Detection of Yersinia pestis, Babesia, Spotted fever group rickettsia, Rickettsia tsutsugamushi, Rickettsia mooseri from rodent's liver and spleen tissues and Borrelia burgdorferi from rodent's Bladder was all negative. Multiple infection was found in some rodents, the infection prevalence was 2.80%. Conclusion Hantavirus, Leptospirosis, Bartonella, Anaplasma phagocytophilum exist in rodent populations that dwell in the volcano lava areas of Xunke county, Heilongjiang, China.

Key words: Rodents, Rodent-borne pathogen, Polymerase chain reaction measuring, Heilongjiang Xunke county, Lava areas

中图分类号: