关键词: 球形芽孢杆菌C_3－41菌株, 晶体和芽孢的分离, 复方泛影葡胺, 二硫苏糖醇

Abstract: A separation procedure of crystals and spores from sporulating cell culture of Bacillus sphaericus C_3-41 was reported in this paper.Spore rate in the preparation was increased to 90-96% by lysozyme treatment and 44% meglumini diatrizoatis composita centrifugation.However,when the strain C-41 grown on the plates of number 6 medium,spore rate in the culture was over 98%.Very pure crystals were separated by a procedure that involves dithiothreitol (DTT)treatment,freezing and thawing of C_3-41 culture as well as 42% meglumini diatrizoatis composita centrifugation.