中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (5): 454-456.

• 论著 • 上一篇    下一篇

分子生物学技术检测鼠形动物巴尔通体

傅桂明1,孙继民1,杨章女1,杨天赐1,斯国静2,庞卫龙3,龚震宇1,刘起勇4   

  1. 1 浙江省疾病预防控制中心病媒生物防制所(杭州 310051); 2 杭州市疾病预防控制中心; 3 天台县疾病预防控制中心; 4 中国疾病预防控制中心传染病预防控制所
  • 收稿日期:2009-06-26 出版日期:2009-10-20 发布日期:2009-10-20
  • 作者简介:傅桂明 (1956-),男,主任技师,主要从事病媒生物与自然疫源性疾病的控制研究。
  • 基金资助:

    浙江省医药卫生科学研究基金(2006B018)

Investigation of Bartonella infection in rats by molecular technology

FU Gui-Ming, SUN Ji-Min, YANG Zhang-Nv, YANG Tian-Ci, SI Guo-Jing, PANG Wei-Long, GONG Zhen-Yu, LIU Qi-Yong   

  1. Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China
  • Received:2009-06-26 Online:2009-10-20 Published:2009-10-20

摘要:

  【摘要】 目的 查明鼠形动物巴尔通体感染情况。 方法 在天台县采用夹夜法捕捉鼠形动物,采集该动物的肝脏,用PCR方法检测DNA,并对阳性产物克隆测序,计算感染情况。结果 采集的55份鼠类肝脏阳性25份,其中黑线姬鼠阳性率为48.84%,黄毛鼠为33.33%,所检测到的巴尔通体与B.doshiae最接近。结论 天台县鼠形动物有很高的巴尔通体DNA阳性率,存在传播给人的风险,必须采取措施加以控制。

关键词: 巴尔通体, 聚合酶链反应, 猫抓病

Abstract:

  【Abstract】 Objective To investigate the infection of Bartonella in rat?shape animals by molecular technology. Methods  Rat?shape animals were captured by night trap in Tiantai county of Zhejiang province. DNA from rat livers were detected by PCR, and the positive products were sequenced. The infection rate of Bartonella in rat was calculated. Results There were 25 positive samples among 55 samples collected. The positive rate of Bartonella infection in Apodemus agrarius and Rattus losea were 48.84% and 33.33%, respectively. The sequence was more similar to B.doshiae. Conclusion The infection rate of Bartonella in rat?shape animals was higher in Tiantai county, and there was risk of transmitting this pathogen to human. The control measure should be figured out. It should take measures to control it.

Key words: Bartonella, Polymerase chain reaction, Cat-scratch disease

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