关键词: 德国小蠊, 近缘种, 形态和分子鉴定

Abstract: Objective To establish morphological and molecular methods for identification of Blattella germanica and its closely related species. Methods External morphology and male genitalia of B. germanica, B. lituricollis, B. bisignata, B. sauteri, and B. singularis were studied in details, the intra-species and inter-species differences of the commonly adopted molecular fragment for species identification-DNA barcodes(cytochrome C oxidase subunitⅠ)were analyzed, neighbor-Joining tree based on these fragments were constructed as well. Results Although the difference of external morphology among B. germanica and its closely related species were not remarkable, and some of the external morphology characteristics between different individuals within the same species were not stable,the differences of the male genitalia among different species were stable and obvious. Intra-species DNA barcode differences of the studied species were less than 2%, and inter-species DNA barcodes differences were more than 2% which is the limitation of DNA barcodes species identification. Conclusion Male genitalia are the golden standard for species morphological identification of B. germanica and its closely related species, DNA barcoding is a good tool for species molecular identification and is supplemental tomorphological identification.

Key words: Blattella germanica, Closely related species, Morphology and molecular identification