中国媒介生物学及控制杂志

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丝光绿蝇抗菌肽的热稳定性研究

许兵红;曾莉萍;赵春澎;董卫华   

  1. 新乡医学院寄生虫学教研室;新乡医学院寄生虫学教研室 新乡
  • 出版日期:2005-08-20 发布日期:2005-08-20

A Study on Antibacterial Activity of Antibacterial Peptide From Lucilia sericata After Treatment at High Temperature

XU Bing-hong; ZENG Li-ping; ZHAO Chun-peng; et al   

  1. Department of Parasitology, Xinxiang Medical College, Xinxiang 453003, China
  • Online:2005-08-20 Published:2005-08-20

摘要: 目的 探讨高温处理对丝光绿蝇抗菌肽抗菌活性的影响。方法 丝光绿蝇Ⅲ龄幼虫针刺体壁诱导后,48h后提取其血淋巴,分别于100、80和60℃水浴0、0.5、1、3、5min等不同时间后,观察不同温度处理不同时间后对溶壁微球菌的抑菌效果。结果 丝光绿蝇幼虫经针刺诱导的抗菌肽100℃水浴1min仍有抗菌活性,100℃处理3min后活性丧失;80及60℃水浴5min后仍有抗菌活性。结论 针刺诱导的丝光绿蝇抗菌肽的抑菌活性具有一定的热稳定性。

关键词: 丝光绿蝇, 抗菌肽, 高温

Abstract: Objective To investigate the stability of antibacterial activity of antibacterial peptide from Lucilia sericata after treatment at high temperature for different time. Methods After being bathed at 100 ℃,80 ℃ and 60 ℃ for 0 sec,30 sec,1 min,3 min and 5 min,the immunized hemolymph were test against Micrococcus lysodeikticus respectively,the bacteriostatic effects were observed after 24 hs and the diameters of clear inhibitory rings were recorded to express antibacterial activity respectively. Results The immunized hemolymph possessed antibacterial activity against M.lysodeikticus after bathed at 100 ℃ for 1 min and also possessed antibacterial activity after bathed at 80 ℃ and 60 ℃ for 5 min. Conclusion The results imply that the induced antibacterial peptide of the blowfly can keep a certain stable antibacterial activity after treatment at high temperature.