关键词: 野鼠动物模型, 戊型肝炎病毒, 子午沙鼠

Abstract: Objective:To set up Laboratory animal model for HEV on wild rodents;merionus meridianus.Methods:Selecting wild rodents,which have been domesticated in the laboratory,infecting to the rodents with HEV in three generations,and examining targets relationship with HEV respectively.In the first generation,the animals were injected by the suspenion liquid of patient waste,who has been confirmed to be infected HEV RNA,and the second and the third generations were infected by the waste of positive rodents for HEV step by step.It was observed for the clinical characters of all test animals,including blood-enzyme characters,blood-toxic characterers,states of discharging HEV,and pathological changes of the histology.Results:There was 20%(2/10) of the clinical characters and 50%(5/10) of mortality rate in Lagurus lagurus in two generations.In Merionus meridianus,to be not obvious clinical characters,0.5%(1/20) of mortality rate,appear special blood-enzyme characters at the sixth day and reinstate gradually at the 48th day after injected,which shown two-peak model.There was 25% of positive for HEV RNA in the waste and 37.5% in the blood in the gerbils.The change characters of liver tissue pathology of the gerbils was similar to the light patients.Conclusion:Depending on the conditions and requirement of laboratory animal models,M.meridianus fits in with HEV laboratory animal models.