关键词: 肾综合征出血热, 快速荧光灶抑制试验, 血清学分型

Abstract: Serotypic classification of 12 HFRS viruses isolated from different sources and patient sera from different areas was studied using rapid fluorescent focus inhibition test (RFFIT).Sera of rabbits inoculated with different HFRS viruses were tested against reference type-ⅠHantaan virus and type-Ⅱ Seoul virus .The neutralizing titers of antisera of Apodemus isolates and patient blood against type-Ⅰ Hantaan virus were 4～256 fold higher than those to type-Ⅱ seoul virus,while the antisera of Rattus isolates-had titers to type-Ⅱ viruses 4～128 fold higher than those to type-Ⅰ viruses.So different isolates could be classified into serotype-Ⅰ and serotype-Ⅱ.In this study,we also found Gou₃ and K₂₄,which could be classified into serotype Ⅱ according to differences (≥4-fold) of the neutralizing titers between the sera reacted against reference type-Ⅰ and type-Ⅱ viruses, which is consistent with data previously published.Otherwise, serotypic classification of patient sera from different areas was investigated. The results showed that the RFFIT is an easy, fast、 specific and exact method and may be used in Serotypic classification of HFRSV.