登革热病毒分子生物学特性及检测方法研究进展

doi:10.11853/j.issn.1003.8280.2019.02.027

中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (2): 224-227.DOI: 10.11853/j.issn.1003.8280.2019.02.027

登革热病毒分子生物学特性及检测方法研究进展

薛志静^1,2, 王君², 宋秀平², 刘小波², 郭玉红², 栗冬梅², 王雪霜², 刘起勇²

1. 泰山医学院基础医学院, 山东泰安 271016;
2. 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206

收稿日期:2018-11-28 出版日期:2019-04-20 发布日期:2019-04-20
通讯作者: 刘起勇,Email:liuqiyong@icdc.cn
作者简介:薛志静,女,在读硕士,主要从事病媒生物与虫媒病研究,Email:573799518@qq.com
基金资助:
国家科技重大专项（2017ZX10303404005001）

Research progress in molecular biological characteristics and detection methods of Dengue virus

XUE Zhi-jing^1,2, WANG Jun², SONG Xiu-ping², LIU Xiao-bo², GUO Yu-hong², LI Dong-mei², WANG Xue-shuang², LIU Qi-yong²

1. Department of Pathogenic Biology of Taishan Medical University, Tai'an, 271016, Shandong Province, China;
2. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention

Received:2018-11-28 Online:2019-04-20 Published:2019-04-20
Supported by:
Supported by the National Science and Technology Major Project of China (No. 2017ZX10303404005001)

摘要/Abstract

摘要： 登革热病毒可以引起登革热、登革出血热和登革休克综合征，主要流行于热带、亚热带地区。近年来，登革热病毒流行范围不断扩大，严重危害人类健康。该文对登革热病毒的分子生物学特性和检测方法的研究进展进行了综述。

关键词: 登革热病毒, 分子生物学特性, 检测方法

Abstract: Dengue virus can cause dengue fever, dengue hemorrhagic fever, and dengue shock syndrome, which is mainly endemic in tropical and subtropical regions. The epidemic area of Dengue virus has been increasing in recent years, which seriously endangers human health. This paper reviews the research progress in molecular biological characteristics and detection methods of Dengue virus.

Key words: Dengue virus, Molecular biological characteristics, Detection method

中图分类号:

R373.3⁺3

薛志静, 王君, 宋秀平, 刘小波, 郭玉红, 栗冬梅, 王雪霜, 刘起勇. 登革热病毒分子生物学特性及检测方法研究进展[J]. 中国媒介生物学及控制杂志, 2019, 30(2): 224-227.

XUE Zhi-jing, WANG Jun, SONG Xiu-ping, LIU Xiao-bo, GUO Yu-hong, LI Dong-mei, WANG Xue-shuang, LIU Qi-yong. Research progress in molecular biological characteristics and detection methods of Dengue virus[J]. Chines Journal of Vector Biology and Control, 2019, 30(2): 224-227.

参考文献

[1] Syed M,Saleem T,Syeda UR,et al.Knowledge,attitudes and practices regarding dengue fever among adults of high and low socioeconomic groups[J].J Pak Med Assoc,2010,60(3):243-247.
[2] Guzman MG,Harris E.Dengue[J].Lancet,2015,385(9966):453-465.DOI:10.1016/S0140-6736(14)60572-9.
[3] Waggoner JJ,Gresh L,Vargas MJ,et al.Viremia and clinical presentation in Nicaraguan patients infected with Zika virus,Chikungunya virus,and Dengue virus[J].Clin Infect Dis,2016,63(12):1584-1590.DOI:10.1093/cid/ciw589.
[4] Ye Z,Huang YX,Jiang P,et al.Analysis of clinical characteristic of 158 inpatients with dengue fever in Guangzhou area during the 2014 epidemic[J].Chin Cri Care Med,2015,27(4):300-305.DOI:10.3760/cma.j.issn.2095-4352.2015.04.015.
[5] Jelinek T.Dengue fever in international travelers[J].Clin Infect Dis,2000,31(1):144-147.DOI:10.1086/313889.
[6] Murrell S,Wu SC,Butler M.Review of Dengue virus and the development of a vaccine[J].Biotechnol Adv,2011,29(2):239-247.DOI:10.1016/j.biotechadv.2010.11.008.
[7] Elghonemy S,Davis WG,Brinton MA.The majority of the nucleotides in the top loop of the genomic 3'terminal stem loop structure are cis-acting in a West Nile virus infectious clone[J].Virology,2005,331(2):238-246.DOI:10.1016/j.virol.2004.11.008.
[8] Kimura-Kuroda J,Yasui K.Antigenic comparison of envelope protein E between Japanese encephalitis virus and some other flaviviruses using monoclonal antibodies[J].J Gen Virol,1986,67(Pt 12):2663-2672.
[9] Chung KM,Nybakken GE,Thompson BS,et al.Antibodies against West Nile virus nonstructural protein NS1 prevent lethal infection through Fcγ receptor-dependent and-independent mechanisms[J].J Virol,2006,80(3):1340-1351.DOI:10.1128/JVI.80.3.1340-1351.2006.
[10] Lin CW,Huang HD,Shiu SY,et al.Functional determinants of NS2B for activation of Japanese encephalitis virus NS3 protease[J].Virus Res,2007,127(1):88-94.DOI:10.1016/j.virusres.2007.03.022.
[11] Khan F,Ahmad A,Ali A,et al.Conformational hotspots of Dengue virus NS5 RdRp[J].Curr Bioinform,2018,13(3):310-318.DOI:10.2174/1574893612666161214124827.
[12] Miller S,Kastner S,Krijnse-Locker J,et al.The non-structural protein 4A of Dengue virus is an integral membrane protein inducing membrane alterations in a 2K-regulated manner[J].J Biol Chem,2007,282(12):8873-8882.DOI:10.1074/jbc.M609919200.
[13] Davidson AD.New insights into Flavivirus Nonstructural protein 5[J].Adv Virus Res,2009,74:41-101.DOI:10.1016/S0065-3527(09)74002-3.
[14] Lin RJ,Chang BL,Yu HP,et al.Blocking of interferon-induced Jak-Stat signaling by Japanese encephalitis virus NS5 through a protein tyrosine phosphatase-mediated mechanism[J].J Virol,2006,80(12):5908-5918.DOI:10.1128/JVI.02714-05.
[15] Jarman RG,Nisalak A,Anderson KB,et al.Factors influencing Dengue virus isolation by C6/36 cell culture and mosquito inoculation of nested PCR-positive clinical samples[J].Am J Trop Med Hyg,2011,84(2):218-223.DOI:10.4269/ajtmh.2011.09-0798.
[16] 吴忠华,罗鹏,徐琦,等.登革病毒检测技术研究进展[J].生物技术通讯,2013(5):727-731.DOI:10.3969/j.issn.1009-0002.2013.05.030.
[17] 盛琳君,李昂,张福杰,等.黄热病病毒检测方法研究进展[J].中华传染病杂志,2017,35(3):190-192.DOI:10.3760/cma.j.issn.1000-6680.2017.03.018.
[18] 王艳红,宝福凯,柳爱华.登革病毒感染的检测技术研究进展[J].生命科学研究,2013,17(1):78-85.DOI:10.3969/j.issn.1007-7847.2013.01.016.
[19] Thiha A,Ibrahim F.A colorimetric Enzyme-Linked immunosorbent assay (ELISA) detection platform for a Point-of-Care dengue detection system on a Lab-on-Compact-Disc[J].Sensors,2015,15(5):11431-11441.DOI:10.3390/s150511431.
[20] Rodríguez Roche R,Alvarez M,Guzmán MG,et al.Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells[J].J Clin Microbiol,2000,38(9):3508-3510.
[21] Muller DA,Depelsenaire ACI,Young PR.Clinical and laboratory diagnosis of Dengue virus infection[J].J Infect Dis,2017,215 Suppl 2:S89-95.DOI:10.1093/infdis/jiw649.
[22] Waggoner JJ,Gresh L,Mohamed-Hadley A,et al.Single-reaction multiplex reverse transcription PCR for detection of Zika,Chikungunya,and Dengue viruses[J].Emerg Infect Dis,2016,22(7):1295-1297.DOI:10.3201/eid2207.160326.
[23] Lanciotti RS,Calisher CH,Gubler DJ,et al.Rapid detection and typing of Dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction[J].J Clin Microbiol,1992,30(3):545-551.
[24] Waggoner JJ,Abeynayake J,Balassiano I,et al.Multiplex nucleic acid amplification test for diagnosis of dengue fever,malaria,and leptospirosis[J].J Clin Microbiol,2014,52(6):2011-2018.DOI:10.1128/JCM.00341-14.
[25] Ahmed NH,Broor S.Comparison of NS1 antigen detection ELISA,real time RT-PCR and virus isolation for rapid diagnosis of dengue infection in acute phase[J].J Vector Borne Dis,2014,51(3):194-199.
[26] Simmons M,Myers T,Guevara C,et al.Development and validation of a quantitative,one-step,multiplex,real-time RT-PCR assay for the detection of dengue and Chikungunya viruses[J].J Clin Microbiol,2016,54(7):1766-1773.DOI:10.1128/JCM.00299-16.
[27] Wu SJL,Lee EM,Putvatana R,et al.Detection of dengue viral RNA using a nucleic acid sequence-based amplification assay[J].J Clin Microbiol,2001,39(8):2794-2798.DOI:10.1128/JCM.39.8.2794-2798.2001.
[28] 庄坚,郭辉玉,陈火胜.非同位素标记探针检测登革病毒RNA的研究[J].中华微生物学和免疫学杂志,1994,14(6):426-429.

登革热病毒分子生物学特性及检测方法研究进展

Research progress in molecular biological characteristics and detection methods of Dengue virus

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

本文评价

[1]	杨瑞军, 黄世腾, 王晓光, 吕磊, 曹国平, 万圣, 叶承华, 陈旭富. 浙江省衢州市1例输入性登革热病例分子流行病学研究[J]. 中国媒介生物学及控制杂志, 2018, 29(5): 445-447.
[2]	马敏, 马晓, 杨思嘉, 王桂安, 孙斌. 登革热病毒的垂直传递和伊蚊滞育研究进展[J]. 中国媒介生物学及控制杂志, 2018, 29(5): 539-542.
[3]	刘晓娜, 吴能简, 吴崧霖, 陈景阳, 谢美莲, 王德全. 深圳市坪山新区2016年登革热媒介监测结果分析[J]. 中国媒介生物学及控制杂志, 2017, 28(3): 283-285.
[4]	李星成, 王志, 戈斌, 孙红专, 冷培恩. 试验现场灭蝇毒饵效果评价及检测方法验证[J]. 中国媒介生物学及控制杂志, 2017, 28(1): 77-78.
[5]	姜进勇, 郭晓芳, 唐烨榕, 杨明东, 魏春, 周红宁. 云南省2004—2014年输入性登革热病例监测与防控对策分析[J]. 中国媒介生物学及控制杂志, 2016, 27(1): 5-8.
[6]	杨鹏飞, 燕清丽, 张丽萍, 聂维忠, 甄维, 马雪征, 白红岩, 刘纯成, 时玉军, 陈跃, 孙肖红, 胡孔新. 秦皇岛口岸首例输入性登革热病例的分子溯源[J]. 中国媒介生物学及控制杂志, 2015, 26(2): 155-158.
[7]	郭鹏娟,甘标,曹钰芹,黄芳,谭锦花,王国玲,曾青华,许少洪. 广州市海珠区2012年登革热流行特征分析[J]. 中国媒介生物学及控制杂志, 2014, 25(1): 76-77.
[8]	白志军, 张培, 洪文艳, 刘建伟, 狄飚, 杨智聪, 任瑞文, 方美玉, 林立辉. 广东省流行的3株登革热2型病毒基因组全序列测定及分析[J]. 中国媒介生物学及控制杂志, 2013, 24(5): 392-396.
[9]	盛子洋, 陈艳雷, 王娟, 吴娜, 范东瀛, 王湘, 高娜, 安静. 登革热病毒SCID-LO2人鼠嵌合模型的建立与评价[J]. 中国媒介生物学及控制杂志, 2012, 23(5): 413-416.
[10]	何金林. 登革热病毒感染标志物检测进展[J]. 中国媒介生物学及控制杂志, 2011, 22(5): 509-511.
[11]	江毅民;严子锵. 我国亚热带地区登革热发生机制[J]. 中国媒介生物学及控制杂志, 2008, 19(1): 80-81.
[12]	冷培恩1;徐劲秋1;邵国财2;朱仁义1;徐仁权1. 灭蝇器灭蝇效果检测方法研究[J]. 中国媒介生物学及控制杂志, 2004, 15(2): 113-114.
[13]	曾晓芃;薛素琴;付学锋. 德国小蠊抗药性检测方法比较研究[J]. 中国媒介生物学及控制杂志, 2004, 15(1): 17-19.
[14]	赵铁镪1; 傅荣华1; 王英军1; 石英1; 杨君兰1; 王志学2; 高桂芬2; 黄智2; 孙百军2; 李淑青2; 吴泽明2; 佟静萍3; 李悦3; 邢铁藩3; 刘庭生3; 王乃忠4; 李晓光4; 曹景坤5; 孙静5; 于泉福6; 何亚轩7; 李志民8. 辽宁省肾综合征出血热流行类型及控制研究[J]. 中国媒介生物学及控制杂志, 1998, 9(2): 119-121.