关键词: 微小按蚊, 线粒体DNA

Abstract: Objective:To study intra-and interspecific molecular variation and phylogeny of four members of the minimus group of Anopheles in Yunnan,which were An.aconitus,An.jeypoirensis,An.minimus species A and An.minimus species C.Method:DNA sequence divergence between these species at mitochondrial locus(cytochrome oxidaseⅠ) is reported.Results:The test confirms the presence of two cryptic An.minmus species A and C,and the relationship between the molecular variation and phylogeny of member of the An.minimus group.DNA sequence diverges between these species at mtDNA,the relationship was very close with An.minimus A and C,An.aconitus near relations was long-term distance,but the An.jeyporiensis was greater distance on divergence.Conclusion:The environment of An.minimus has an effect on genetic variations of mtDNA,but the difference geographic population of An.minimus was not much changing at genetic distance.