关键词: 汉坦病毒, 肾综合征出血热, 基因, 分型, 核苷酸

Abstract: Objective:To understand the distribute of infection of Hantaviruses in rodents and it's genetic type and sero-type of patients of Hemorrhagic fever with renal syndrome(HFRS) in Beijing.To determine the nature of infections focus and further provide the scientific basis for how to prevent the disease.Method:Using Immunofluorescent assay test(IFAT) to testing the infectious state of Hantaviruses in lungs of rodents captured in Beijing in 1996-1999.21 Hantavirus RAN,positive with Hantavirus antigens with frozen sectioning and IFAT,were reverse-transcribed and amplified with PCR with Hantavirus-specific primers.Using MCPENT to detect the serum of 55 patients with HFRS in acute period and typing them.Results:The infection of Hantavirus widely exists in rodents in Beijing.The rate of Hantavirus carried is 1.89%-6.17%.21 Hantavirus RNA isolated from lungs of rodents,positive with Hantavirus antigens with frozen sectioning and Immunofluorescent assay,were rever-transcribed We respectively used the SEO type-specfic primers(SEO MF and SEO MR) and HTN type-primers(HMF and HMR) in RT-PCR amplify 21 samples.The specific segments that molecular weight is 330bp were obtained by using the former primers.The amplify result of the later primers were negative.We retrieved the fragment of PCR products of 9 samples and determined their nucleotide sequences.The nucleotide sequence of these samples were compared with SEO type Hantavirus L₉₉ and SEO,the homology is 94.3%-100%.But they were compared with HTN type Hantavirus A₉,the homology of nucleotide sequence is less than 71%.So the nine virus strains are all SEO type hantavirus.But there are different in nine samples.Phylogenetic tree analysis showed that the nine strain viruses were divided into at least four branches.We discovered greater variations in nucleotide sequence of B₁₀.It is possible a new sub-type of SEO.The homology among BF₁₀ and L₉₉ is 100% and among BF₇and G₁₉₉ is 99.3%.Whether it is indicate long-distance spread still need to further study.33 of 55 patients are caused by SEO type hantavirus and 2 antibody of type Ⅰ and type Ⅱin serum are all 80,so we didn't determine the type of these patients.Conclusion:SEO type hantavirus strain is the main epidemic strain in Beijing in recent years,SEO type hantavirus is the infection factor caused the patients incrased.