中国媒介生物学及控制杂志

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肾综合征出血热病毒在革螨体内增殖的动态观察

张云; 朱进; 吴光华   

  1. 南京军区军事医学研究所 南京210002
  • 出版日期:2000-12-20 发布日期:2000-12-20

Dynamic Observation on Proliferation of Hemorrhagic Fever with Renal Syndrome Virus in Gasmid Mite

Zhang Yun; Zhu Jin; Wu Guanghua   

  1. Institute of Military Medicine, Nanjing Command, Nanjing 210002
  • Online:2000-12-20 Published:2000-12-20

摘要: 目的:进一步观察革螨体内肾综合征出血热病毒(HFRSV)有否增殖。方法:用直接细胞培养动态观察革螨体内HFRSV滴度。结果:发现鼠肺HFRSV抗原阳性和阴性革螨,均在不同子代革螨体内测出HFRSV滴度(10-1~10-6);动态观察发现子代革螨病毒滴度较母代革螨高10-3~10-5。用抗Mp和Np结构蛋白单克隆抗体检测发现,随着病毒滴度增高而毒力增强。结论:该研究结果从病毒学和分子生物学上进一步证明HFRSV可在革螨体内分布、增殖,并可经代传递。

关键词: 革螨, 肾综合征出血热, 病毒

Abstract: Objective: It was to further observe dynamic changes on proliferation of hemorrhagic fever with renal syndrome virus (HFRSV) in gasmid mite. Methods: The titres of HFRSV in gasmid mite were dynamic observed from cultured cells. Results: The titres of HFRSV were detected in different generation of gasmid mite which separated from HFRSV antigen positive or negative mice, the titres were in 10-1~10-6,but the titre of generation was as more as 10-3~10-5 than the last generation. It was found by anti-Mp/Np McAb that the higher the titre of virus was, the higher the toxicity was. Conclusion: The results demonstrate that the HFRSV could proliferate in gasmid mite and be transmitted by trans-stadial.