中国媒介生物学及控制杂志

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应用半巢式PCR检测我国北方一些蜱种中的查菲埃立克体

高东旗1; 曹务春1; 张习坦1; 赵秋敏1; 朱建华2; 方立群1   

  1. 1军事医学科学院微生物流行病研究所 北京100071; 2内蒙古大兴安岭林业中心防疫站
  • 出版日期:2000-06-20 发布日期:2000-06-20

Detection of Ehrlichia chaffeensis by Semi PCR from Ticks in the North of China

Gao Dongqi; Cao Wuchun; Zhang Xitan; et al   

  1. Institute of Microbiology & Epidemiology,Academy of Military Medical Science, Beijing 100071
  • Online:2000-06-20 Published:2000-06-20

摘要: 目的:了解我国北方一些蜱种中是否携带查菲埃立克体(Ehrlichiachaffeensis,简写为EC)。方法:应用半巢式PCR检测蜱的带菌率,利用16SrRNA基因测序方法鉴定所测序列。结果:从内蒙古莫尔道嘎林业局采集的全沟硬蜱和森林革蜱中扩增出查菲埃立克体的16SrRNA基因,阳性率分别是39.06%和10.00%;并从新疆精河采集的全沟硬蜱和草原革蜱中也测到相同的序列,最小阳性率分别为5.79%和1.67%。对莫尔道嘎采集的蜱标本进行1220bp的EC16SrRNA基因分析,结果与美国1株查菲埃立克体(GenBankU23503)的相应序列只差1个碱基。结论:所使用的两套半巢式PCR扩增引物,可以简便快速地检测和分析蜱标本中可能存在的EC。

关键词: 半巢式PCR, 查菲埃立克体, 蜱, 16SrRNA基因

Abstract: Ehrlichia chaffeensis (EC) 16S rRNA was amplified for the 1st time from Ixodes persulcatus and Dermacenter silvarum in China. The EC infection rate of 39.1 % in I. persulcatus and 10 % in D. silvarum at MoRidaogo of Inner Mongolia, and minimum infection rates of 5.79 % in I. persulcatus and 1.67 % in D. nutalli at Jinghe of Xinjiang were detected. Sequence analysis of PCR products indicated that the 1 220 bp EC 16S rRNA gene amplified from ticks collected at MoRidaogo differed an American EC isolate (GenBank U23503) by only one base pair.