关键词: 生物素DNA探针, PCR, 班氏丝虫, 蚊媒监测

Abstract: AIM: To evaluate the usefulness of biotin-DNA probe/PCR system for post control surveillance of Bancroftian filariasis in mosquito. Methods: This assay was used to detect the mosquitoes infected in a laboratory and in an endemic area by the way of W.bancrofti specific DNA probe labelled with photobiotin, after samples amplified by PCR. Results: This probe hybridized with DNA of W.bancrofti only, not with DNAs of B. malayi , some animal filariae and normal mosquitoes. Among the same batch mosquitoes artificially infected with W.bancrofti, the positive rates were 30.67 % by probe, and 21.28 % by dissectting microscopy. Under infectiosity of 1.6 filarial larvae/mosquito, it was detectable when 1 out of 50 mosquitoes was infected. When each group with 20 mosquitoes was detected by the system of DNA probe/PCR system, the lowest positive rates of mosquitoes caught from patients and non patientshouse in the same village of Henan province(W.bancrofti endemic area) were 0.75 % and 0.19 % respectively, but the mosquitoes caught from non patient village were all negative. Conclusion: Results show that the method had reached the practical level because its high sensitivity and specifty and also proved a new useful tool for the surveillance of filariasis in the late stage control.