中国媒介生物学及控制杂志

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鼠疫耶尔森氏菌16S-23S rRNA基因间区的酶切位点分析

魏建春; 周艳; 刘梅; 蔡虹; 俞东征   

  1. 中国预防医学科学院流行病学微生物学研究所 北京102206
  • 出版日期:2000-06-20 发布日期:2000-06-20

Analysis on the Physical Pattern of 16S-23S rRNA Spacer Region of Y.pestis

Wei Jianchun; Zhou Yan; Liu Mei; et al   

  1. Institute of Epidemiology and Microbiology, CAPM,Beijing 102206
  • Online:2000-06-20 Published:2000-06-20

摘要: 目的:了解鼠疫耶尔森氏菌16S-23S rRNA基因间区的酶切位点分布情况。方法:PCR扩增及限制性内切酶分析。结果:对EV菌及不同来源的103株鼠疫耶尔森氏菌16S-23S rRNA基因间区进行了扩增,选用限制性内切酶TaqⅠ及MspⅠ对扩增产物进行酶切分析,结果发现所用鼠疫耶尔森氏菌该间区扩增产物及酶切图谱一致;选用TaqⅠ+MspⅠ,HinfⅠ+MspⅠ对EV菌16S-23S rRNA基因间区扩增产物进行了双酶切分析,作出了TaqⅠ,MspⅠ,Hinf Ⅰ在EV菌该间区的酶切位点图。结论:鼠疫耶尔森氏菌16S-23S rRNA基因间区相当保守;酶切位点分析为对该区进行进一步研究打下了基础。

关键词: 鼠疫耶尔森氏菌, rRNA基因

Abstract: In this study the spacer region between the genes coding for 16S-23S rRNAs and a portion of the gene coding for 23S rRNA (spacer+23S) were selected for analysis. 103 Y.pestis strains that isolated from 9 different gorups of natural foci and belong to 21 ecotypes were studied. It was found that all of the tested strains showed two identical fragments of 1 146bp and 1 090bp. The amplified products were digested with two restriction endonucleases Taq Ⅰ and Msp Ⅰ,the result showed that the restriction patterns were identical. The physical map of 16S-23S rRNA gene spacer region of EV strains with the restriction endonucleases Taq Ⅰ, Msp Ⅰ and Hinf Ⅰ were constructed, it might be useful for further study of this region.