中国媒介生物学及控制杂志

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从黑龙江小兴安岭棕背(鼠平)和花鼠分离到莱姆病螺旋体

万康林1; 张哲夫1; 贾文春2; 张淑芹2; 张晓杰2; 宋鸿章2; 木井文2; 张金声1   

  1. 1中国预防医学科学院流行病学微生物学研究所; 2黑龙江省伊春市卫生防疫站
  • 出版日期:1997-06-20 发布日期:1997-06-20

Borrelia burgdorferi Isolated from Clethrionomys rufocanus and Eutamias sibricus in the Small Xing-An Moumtains of Heilongjiang Province, China

Wan Kang-lin*; Zhang Zhe-fu*; Jia Wen-Chun; et al   

  1. Institute of Epidemiology and Microbiology,Chinese Academy of Preventive Medicine,Beijing,China
  • Online:1997-06-20 Published:1997-06-20

摘要: 目的:为初步掌握黑龙江省小兴安岭林区动物莱姆病分布情况。方法:于1995年7月用鼠笼捕获野鼠30只,采用间接免疫荧光抗体法调查鼠血清抗莱姆病螺旋体抗体。用BSK培养基从鼠脏器分离病原。结果:阳性共8只,阳性率26.67%;其中优势种属野鼠阳性率达31.36%;从棕背和花鼠各分离出1株莱姆病螺旋体。新分离株的单克隆抗体反应与国内分离株SR1相同,与美国B31有区别,对H6831呈阴性反应。结论:鼠型啮齿动物可能是莱姆病螺旋体的动物宿主,属野鼠则是重要动物宿主。

关键词: 莱姆病螺旋体, 棕背(鼠平), 动物宿主

Abstract: In July, 1995, the animal reservoirs of Lyme disease spirochetes was investigated in the Small Xing-An Mountains of Heilongjiang province, China.of 30 rats, 8 rats had a signifcant antibody titer against Borrelia burgdorferi and Clethrionomys rufocanus was prevailing in this region.Two Strains of Borrelia burgdorferi were cultivated from the kidney of Clehrionomys rufocanus and the bladder of Eutamias sibricus and these isolates were distinguished between China and USA by monoclonal antibodies (H5332, H6831 and H9724).These studies indicated that Clethrionomys rufocanus be primary reservoir of Borrelia burgdorferi and this region be an endemic area of Lyme disease.