关键词: 蚊细胞, 髓机引物扩增多态性DNA聚合酶链反应

Abstract: Primer Pm was screened by RAPD-PCR. Four seperate DNA maps were obtained through amplifing the genom DNA of the mosquito cells (Anso-242,Cpp-512, Ana-104, C6/36) with the primer Pm.The molecular weights (kb) of the DNA bands of each map: Anso-242 0.50, 0.88, 1.02, 1.13, 1.22, 1.5, 2.1, 2.4, 2.5;Cpp-512 0.5, 0.7, 0.8, 0.88, 1.02, 1.22; Ana-104 0.64, 0.98, 1.2, 1.28, 1.4, 1.53,1.8,2.3, 2.6, 3.3; C6/36 0.66, 0.8, 0.94, 1.4, 1.42, 1.5, 1.52, 2.9. The test result showed that RAPD-PCR method is simple and ,feasible for biological classification.The results are stable and reproducible.The Primer Pm may satisfy the needs for the identification of the four mosquito cells.