Abstract: Objective To analyze the molecular genetic characteristics of Hantavirus strain DX1101 isolated from the imported Rattus norvegicus. Methods We separated the rat lung, extracted virus RNA, and then used codehop RT-PCR to amplify L gene segment of Hantavirus and nested RT-PCR for M gene segment. The obtained products were then sequenced and analysed by phylogenetic tree. Results Homological and phylogenetic analysis of the two gene amplification products showed that the Hantavirus strain belongs to genetic subtype SEOV. The phylogenetic tree of M gene showed that the strain is genetically closest to the strain IR461 found in England. Conclusion This study identified Hantavirus in R. norvegicus seized from inboard containers. Therefore, it is of great significance to enhance the quarantine of inboard containers.

Key words: Hantavirus, Imported rat, Molecular genetic analysis

摘要： 目的 对1例在境外输入褐家鼠中分离的汉坦病毒DX1101株进行分子遗传学特征分析。方法 剖取鼠肺组织,提取病毒RNA,用简并RT-PCR和巢式RT-PCR分别扩增汉坦病毒L基因和M基因片段,对扩增产物测序并构建系统发生树进行分型与系统发生分析。结果 2个基因的扩增片段序列同源性系统发生树分析均显示该病毒株为汉城型汉坦病毒;M基因的系统进化树显示与英国分离株IR461基因距离最为接近。结论 证实入境集装箱中截获的鼠类携带汉坦病毒,加强入境集装箱的卫生检疫有重要意义。

关键词: 汉坦病毒, 输入性, 遗传学分析